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Serum Metabolomic Profiling of Patients with Non-Infectious Uveitis

The activities of various metabolic pathways can influence the pathogeneses of autoimmune diseases, and intrinsic metabolites can potentially be used to diagnose diseases. However, the metabolomic analysis of patients with uveitis has not yet been conducted. Here, we profiled the serum metabolomes o...

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Autores principales: Shimizu, Hiroyuki, Usui, Yoshihiko, Asakage, Masaki, Nezu, Naoya, Wakita, Ryo, Tsubota, Kinya, Sugimoto, Masahiro, Goto, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7762156/
https://www.ncbi.nlm.nih.gov/pubmed/33291298
http://dx.doi.org/10.3390/jcm9123955
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author Shimizu, Hiroyuki
Usui, Yoshihiko
Asakage, Masaki
Nezu, Naoya
Wakita, Ryo
Tsubota, Kinya
Sugimoto, Masahiro
Goto, Hiroshi
author_facet Shimizu, Hiroyuki
Usui, Yoshihiko
Asakage, Masaki
Nezu, Naoya
Wakita, Ryo
Tsubota, Kinya
Sugimoto, Masahiro
Goto, Hiroshi
author_sort Shimizu, Hiroyuki
collection PubMed
description The activities of various metabolic pathways can influence the pathogeneses of autoimmune diseases, and intrinsic metabolites can potentially be used to diagnose diseases. However, the metabolomic analysis of patients with uveitis has not yet been conducted. Here, we profiled the serum metabolomes of patients with three major forms of uveitis (Behҫet’s disease (BD), sarcoidosis, and Vogt-Koyanagi-Harada disease (VKH)) to identify potential biomarkers. This study included 19 BD, 20 sarcoidosis, and 15 VKH patients alongside 16 healthy control subjects. The metabolite concentrations in their sera were quantified using liquid chromatography with time-of-flight mass spectrometry. The discriminative abilities of quantified metabolites were evaluated by four comparisons: control vs. three diseases, and each disease vs. the other two diseases (such as sarcoidosis vs. BD + VKH). Among 78 quantified metabolites, 24 kinds of metabolites showed significant differences in these comparisons. Four multiple logistic regression models were developed and validated. The area under the receiver operating characteristic (ROC) curve (AUC) in the model to discriminate disease groups from control was 0.72. The AUC of the other models to discriminate sarcoidosis, BD, and VKH from the other two diseases were 0.84, 0.83, and 0.73, respectively. This study provides potential diagnostic abilities of sarcoidosis, BD, and VKH using routinely available serum samples that can be collected with minimal invasiveness.
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spelling pubmed-77621562020-12-26 Serum Metabolomic Profiling of Patients with Non-Infectious Uveitis Shimizu, Hiroyuki Usui, Yoshihiko Asakage, Masaki Nezu, Naoya Wakita, Ryo Tsubota, Kinya Sugimoto, Masahiro Goto, Hiroshi J Clin Med Article The activities of various metabolic pathways can influence the pathogeneses of autoimmune diseases, and intrinsic metabolites can potentially be used to diagnose diseases. However, the metabolomic analysis of patients with uveitis has not yet been conducted. Here, we profiled the serum metabolomes of patients with three major forms of uveitis (Behҫet’s disease (BD), sarcoidosis, and Vogt-Koyanagi-Harada disease (VKH)) to identify potential biomarkers. This study included 19 BD, 20 sarcoidosis, and 15 VKH patients alongside 16 healthy control subjects. The metabolite concentrations in their sera were quantified using liquid chromatography with time-of-flight mass spectrometry. The discriminative abilities of quantified metabolites were evaluated by four comparisons: control vs. three diseases, and each disease vs. the other two diseases (such as sarcoidosis vs. BD + VKH). Among 78 quantified metabolites, 24 kinds of metabolites showed significant differences in these comparisons. Four multiple logistic regression models were developed and validated. The area under the receiver operating characteristic (ROC) curve (AUC) in the model to discriminate disease groups from control was 0.72. The AUC of the other models to discriminate sarcoidosis, BD, and VKH from the other two diseases were 0.84, 0.83, and 0.73, respectively. This study provides potential diagnostic abilities of sarcoidosis, BD, and VKH using routinely available serum samples that can be collected with minimal invasiveness. MDPI 2020-12-06 /pmc/articles/PMC7762156/ /pubmed/33291298 http://dx.doi.org/10.3390/jcm9123955 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shimizu, Hiroyuki
Usui, Yoshihiko
Asakage, Masaki
Nezu, Naoya
Wakita, Ryo
Tsubota, Kinya
Sugimoto, Masahiro
Goto, Hiroshi
Serum Metabolomic Profiling of Patients with Non-Infectious Uveitis
title Serum Metabolomic Profiling of Patients with Non-Infectious Uveitis
title_full Serum Metabolomic Profiling of Patients with Non-Infectious Uveitis
title_fullStr Serum Metabolomic Profiling of Patients with Non-Infectious Uveitis
title_full_unstemmed Serum Metabolomic Profiling of Patients with Non-Infectious Uveitis
title_short Serum Metabolomic Profiling of Patients with Non-Infectious Uveitis
title_sort serum metabolomic profiling of patients with non-infectious uveitis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7762156/
https://www.ncbi.nlm.nih.gov/pubmed/33291298
http://dx.doi.org/10.3390/jcm9123955
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