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Explorative Screening of Bioactivities Generated by Plant-Based Proteins after In Vitro Static Gastrointestinal Digestion

The gastrointestinal digestion of food proteins can generate peptides with a wide range of biological activities. In this study, we screened various potential bioactivities generated by plant-based proteins. Whey protein as an animal protein reference, five grades of pea protein, two grades of wheat...

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Autores principales: Dugardin, Camille, Cudennec, Benoit, Tourret, Mélissa, Caron, Juliette, Guérin-Deremaux, Laetitia, Behra-Miellet, Josette, Lefranc-Millot, Catherine, Ravallec, Rozenn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7762166/
https://www.ncbi.nlm.nih.gov/pubmed/33291464
http://dx.doi.org/10.3390/nu12123746
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author Dugardin, Camille
Cudennec, Benoit
Tourret, Mélissa
Caron, Juliette
Guérin-Deremaux, Laetitia
Behra-Miellet, Josette
Lefranc-Millot, Catherine
Ravallec, Rozenn
author_facet Dugardin, Camille
Cudennec, Benoit
Tourret, Mélissa
Caron, Juliette
Guérin-Deremaux, Laetitia
Behra-Miellet, Josette
Lefranc-Millot, Catherine
Ravallec, Rozenn
author_sort Dugardin, Camille
collection PubMed
description The gastrointestinal digestion of food proteins can generate peptides with a wide range of biological activities. In this study, we screened various potential bioactivities generated by plant-based proteins. Whey protein as an animal protein reference, five grades of pea protein, two grades of wheat protein, and potato, fava bean, and oat proteins were submitted to in vitro SGID. They were then tested in vitro for several bioactivities including measures on: (1) energy homeostasis through their ability to modulate intestinal hormone secretion, to inhibit DPP-IV activity, and to interact with opioid receptors; (2) anti-hypertensive properties through their ability to inhibit ACE activity; (3) anti-inflammatory properties in Caco-2 cells; (4) antioxidant properties through their ability to inhibit production of reactive oxygen species (ROS). Protein intestinal digestions were able to stimulate intestinal hormone secretion by enteroendocrine cells, to inhibit DPP-IV and ACE activities, to bind opioid receptors, and surprisingly, to decrease production of ROS. Neither pro- nor anti-inflammatory effects have been highlighted and some proteins lost their pro-inflammatory potential after digestion. The best candidates were pea, potato, and fava bean proteins.
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spelling pubmed-77621662020-12-26 Explorative Screening of Bioactivities Generated by Plant-Based Proteins after In Vitro Static Gastrointestinal Digestion Dugardin, Camille Cudennec, Benoit Tourret, Mélissa Caron, Juliette Guérin-Deremaux, Laetitia Behra-Miellet, Josette Lefranc-Millot, Catherine Ravallec, Rozenn Nutrients Article The gastrointestinal digestion of food proteins can generate peptides with a wide range of biological activities. In this study, we screened various potential bioactivities generated by plant-based proteins. Whey protein as an animal protein reference, five grades of pea protein, two grades of wheat protein, and potato, fava bean, and oat proteins were submitted to in vitro SGID. They were then tested in vitro for several bioactivities including measures on: (1) energy homeostasis through their ability to modulate intestinal hormone secretion, to inhibit DPP-IV activity, and to interact with opioid receptors; (2) anti-hypertensive properties through their ability to inhibit ACE activity; (3) anti-inflammatory properties in Caco-2 cells; (4) antioxidant properties through their ability to inhibit production of reactive oxygen species (ROS). Protein intestinal digestions were able to stimulate intestinal hormone secretion by enteroendocrine cells, to inhibit DPP-IV and ACE activities, to bind opioid receptors, and surprisingly, to decrease production of ROS. Neither pro- nor anti-inflammatory effects have been highlighted and some proteins lost their pro-inflammatory potential after digestion. The best candidates were pea, potato, and fava bean proteins. MDPI 2020-12-05 /pmc/articles/PMC7762166/ /pubmed/33291464 http://dx.doi.org/10.3390/nu12123746 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dugardin, Camille
Cudennec, Benoit
Tourret, Mélissa
Caron, Juliette
Guérin-Deremaux, Laetitia
Behra-Miellet, Josette
Lefranc-Millot, Catherine
Ravallec, Rozenn
Explorative Screening of Bioactivities Generated by Plant-Based Proteins after In Vitro Static Gastrointestinal Digestion
title Explorative Screening of Bioactivities Generated by Plant-Based Proteins after In Vitro Static Gastrointestinal Digestion
title_full Explorative Screening of Bioactivities Generated by Plant-Based Proteins after In Vitro Static Gastrointestinal Digestion
title_fullStr Explorative Screening of Bioactivities Generated by Plant-Based Proteins after In Vitro Static Gastrointestinal Digestion
title_full_unstemmed Explorative Screening of Bioactivities Generated by Plant-Based Proteins after In Vitro Static Gastrointestinal Digestion
title_short Explorative Screening of Bioactivities Generated by Plant-Based Proteins after In Vitro Static Gastrointestinal Digestion
title_sort explorative screening of bioactivities generated by plant-based proteins after in vitro static gastrointestinal digestion
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7762166/
https://www.ncbi.nlm.nih.gov/pubmed/33291464
http://dx.doi.org/10.3390/nu12123746
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