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Improving the Intercellular Uptake and Osteogenic Potency of Calcium Phosphate via Nanocomplexation with the RALA Peptide

Calcium phosphate-base materials (e.g., alpha tri-calcium phosphate (α–TCP)) have been shown to promote osteogenic differentiation of stem/progenitor cells, enhance osteoblast osteogenic activity and mediate in vivo bone tissue formation. However, variable particle size and hydrophilicity of the cal...

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Autores principales: O’Doherty, Michelle, Mulholland, Eoghan J., Chambers, Philip, Pentlavalli, Sreekanth, Ziminska, Monika, Chalanqui, Marine J., Pauly, Hannah M., Sathy, Binulal N., Donahue, Tammy H., Kelly, Daniel J., Dunne, Nicholas, McCarthy, Helen O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7762210/
https://www.ncbi.nlm.nih.gov/pubmed/33297306
http://dx.doi.org/10.3390/nano10122442
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author O’Doherty, Michelle
Mulholland, Eoghan J.
Chambers, Philip
Pentlavalli, Sreekanth
Ziminska, Monika
Chalanqui, Marine J.
Pauly, Hannah M.
Sathy, Binulal N.
Donahue, Tammy H.
Kelly, Daniel J.
Dunne, Nicholas
McCarthy, Helen O.
author_facet O’Doherty, Michelle
Mulholland, Eoghan J.
Chambers, Philip
Pentlavalli, Sreekanth
Ziminska, Monika
Chalanqui, Marine J.
Pauly, Hannah M.
Sathy, Binulal N.
Donahue, Tammy H.
Kelly, Daniel J.
Dunne, Nicholas
McCarthy, Helen O.
author_sort O’Doherty, Michelle
collection PubMed
description Calcium phosphate-base materials (e.g., alpha tri-calcium phosphate (α–TCP)) have been shown to promote osteogenic differentiation of stem/progenitor cells, enhance osteoblast osteogenic activity and mediate in vivo bone tissue formation. However, variable particle size and hydrophilicity of the calcium phosphate result in an extremely low bioavailability. Therefore, an effective delivery system is required that can encapsulate the calcium phosphate, improve cellular entry and, consequently, elicit a potent osteogenic response in osteoblasts. In this study, collagenous matrix deposition and extracellular matrix mineralization of osteoblast lineage cells were assessed to investigate osteogenesis following intracellular delivery of α-TCP nanoparticles. The nanoparticles were formed via condensation with a novel, cationic 30 mer amphipathic peptide (RALA). Nanoparticles prepared at a mass ratio of 5:1 demonstrated an average particle size of 43 nm with a zeta potential of +26 mV. The average particle size and zeta potential remained stable for up to 28 days at room temperature and across a range of temperatures (4–37 °C). Cell viability decreased 24 h post-transfection following RALA/α-TCP nanoparticle treatment; however, recovery ensued by Day 7. Immunocytochemistry staining for Type I collagen up to Day 21 post-transfection with RALA/α-TCP nanoparticles (NPs) in MG-63 cells exhibited a significant enhancement in collagen expression and deposition compared to an untreated control. Furthermore, in porcine mesenchymal stem cells (pMSCs), there was enhanced mineralization compared to α–TCP alone. Taken together these data demonstrate that internalization of RALA/α-TCP NPs elicits a potent osteogenic response in both MG-63 and pMSCs.
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spelling pubmed-77622102020-12-26 Improving the Intercellular Uptake and Osteogenic Potency of Calcium Phosphate via Nanocomplexation with the RALA Peptide O’Doherty, Michelle Mulholland, Eoghan J. Chambers, Philip Pentlavalli, Sreekanth Ziminska, Monika Chalanqui, Marine J. Pauly, Hannah M. Sathy, Binulal N. Donahue, Tammy H. Kelly, Daniel J. Dunne, Nicholas McCarthy, Helen O. Nanomaterials (Basel) Article Calcium phosphate-base materials (e.g., alpha tri-calcium phosphate (α–TCP)) have been shown to promote osteogenic differentiation of stem/progenitor cells, enhance osteoblast osteogenic activity and mediate in vivo bone tissue formation. However, variable particle size and hydrophilicity of the calcium phosphate result in an extremely low bioavailability. Therefore, an effective delivery system is required that can encapsulate the calcium phosphate, improve cellular entry and, consequently, elicit a potent osteogenic response in osteoblasts. In this study, collagenous matrix deposition and extracellular matrix mineralization of osteoblast lineage cells were assessed to investigate osteogenesis following intracellular delivery of α-TCP nanoparticles. The nanoparticles were formed via condensation with a novel, cationic 30 mer amphipathic peptide (RALA). Nanoparticles prepared at a mass ratio of 5:1 demonstrated an average particle size of 43 nm with a zeta potential of +26 mV. The average particle size and zeta potential remained stable for up to 28 days at room temperature and across a range of temperatures (4–37 °C). Cell viability decreased 24 h post-transfection following RALA/α-TCP nanoparticle treatment; however, recovery ensued by Day 7. Immunocytochemistry staining for Type I collagen up to Day 21 post-transfection with RALA/α-TCP nanoparticles (NPs) in MG-63 cells exhibited a significant enhancement in collagen expression and deposition compared to an untreated control. Furthermore, in porcine mesenchymal stem cells (pMSCs), there was enhanced mineralization compared to α–TCP alone. Taken together these data demonstrate that internalization of RALA/α-TCP NPs elicits a potent osteogenic response in both MG-63 and pMSCs. MDPI 2020-12-07 /pmc/articles/PMC7762210/ /pubmed/33297306 http://dx.doi.org/10.3390/nano10122442 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
O’Doherty, Michelle
Mulholland, Eoghan J.
Chambers, Philip
Pentlavalli, Sreekanth
Ziminska, Monika
Chalanqui, Marine J.
Pauly, Hannah M.
Sathy, Binulal N.
Donahue, Tammy H.
Kelly, Daniel J.
Dunne, Nicholas
McCarthy, Helen O.
Improving the Intercellular Uptake and Osteogenic Potency of Calcium Phosphate via Nanocomplexation with the RALA Peptide
title Improving the Intercellular Uptake and Osteogenic Potency of Calcium Phosphate via Nanocomplexation with the RALA Peptide
title_full Improving the Intercellular Uptake and Osteogenic Potency of Calcium Phosphate via Nanocomplexation with the RALA Peptide
title_fullStr Improving the Intercellular Uptake and Osteogenic Potency of Calcium Phosphate via Nanocomplexation with the RALA Peptide
title_full_unstemmed Improving the Intercellular Uptake and Osteogenic Potency of Calcium Phosphate via Nanocomplexation with the RALA Peptide
title_short Improving the Intercellular Uptake and Osteogenic Potency of Calcium Phosphate via Nanocomplexation with the RALA Peptide
title_sort improving the intercellular uptake and osteogenic potency of calcium phosphate via nanocomplexation with the rala peptide
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7762210/
https://www.ncbi.nlm.nih.gov/pubmed/33297306
http://dx.doi.org/10.3390/nano10122442
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