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HIV-1C env and gag Variation in the Cerebrospinal Fluid and Plasma of Patients with HIV-Associated Cryptococcal Meningitis in Botswana

HIV-1 compartmentalization in reservoir sites remains a barrier to complete HIV eradication. It is unclear whether there is variation in HIV-1 env and gag between cerebrospinal fluid (CSF) and plasma of individuals with HIV-associated cryptococcal meningitis (CM). We compared HIV-1 env characteristi...

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Autores principales: Kelentse, Nametso, Moyo, Sikhulile, Mogwele, Mompati L., Ditshwanelo, Doreen, Mokaleng, Baitshepi, Moraka, Natasha O., Lechiile, Kwana, Leeme, Tshepo B., Lawrence, David S., Musonda, Rosemary, Kasvosve, Ishmael, Harrison, Thomas S., Jarvis, Joseph N., Gaseitsiwe, Simani
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7762280/
https://www.ncbi.nlm.nih.gov/pubmed/33297399
http://dx.doi.org/10.3390/v12121404
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author Kelentse, Nametso
Moyo, Sikhulile
Mogwele, Mompati L.
Ditshwanelo, Doreen
Mokaleng, Baitshepi
Moraka, Natasha O.
Lechiile, Kwana
Leeme, Tshepo B.
Lawrence, David S.
Musonda, Rosemary
Kasvosve, Ishmael
Harrison, Thomas S.
Jarvis, Joseph N.
Gaseitsiwe, Simani
author_facet Kelentse, Nametso
Moyo, Sikhulile
Mogwele, Mompati L.
Ditshwanelo, Doreen
Mokaleng, Baitshepi
Moraka, Natasha O.
Lechiile, Kwana
Leeme, Tshepo B.
Lawrence, David S.
Musonda, Rosemary
Kasvosve, Ishmael
Harrison, Thomas S.
Jarvis, Joseph N.
Gaseitsiwe, Simani
author_sort Kelentse, Nametso
collection PubMed
description HIV-1 compartmentalization in reservoir sites remains a barrier to complete HIV eradication. It is unclear whether there is variation in HIV-1 env and gag between cerebrospinal fluid (CSF) and plasma of individuals with HIV-associated cryptococcal meningitis (CM). We compared HIV-1 env characteristics and the gag cytotoxic T-lymphocyte (CTL) escape mutations from CSF and plasma samples. Employing population-based Sanger sequencing, we sequenced HIV-1 env from CSF of 25 patients and plasma of 26 patients. For gag, 15 CSF and 21 plasma samples were successfully sequenced. Of these, 18 and 9 were paired env and gag CSF/plasma samples, respectively. There was no statistically significant difference in the proportion of CCR5-using strains in the CSF and plasma, (p = 0.50). Discordant CSF/plasma virus co-receptor use was found in 2/18 pairs (11.1%). The polymorphisms in the HIV-1 V3 loop were concordant between the two compartments. From the HIV-1 gag sequences, three pairs had discordant CTL escape mutations in three different epitopes of the nine analyzed. These findings suggest little variation in the HIV-1 env between plasma and CSF and that the CCR5-using strains predominate in both compartments. HIV-1 gag CTL escape mutations also displayed little variation in CSF and plasma suggesting similar CTL selective pressure.
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spelling pubmed-77622802020-12-26 HIV-1C env and gag Variation in the Cerebrospinal Fluid and Plasma of Patients with HIV-Associated Cryptococcal Meningitis in Botswana Kelentse, Nametso Moyo, Sikhulile Mogwele, Mompati L. Ditshwanelo, Doreen Mokaleng, Baitshepi Moraka, Natasha O. Lechiile, Kwana Leeme, Tshepo B. Lawrence, David S. Musonda, Rosemary Kasvosve, Ishmael Harrison, Thomas S. Jarvis, Joseph N. Gaseitsiwe, Simani Viruses Article HIV-1 compartmentalization in reservoir sites remains a barrier to complete HIV eradication. It is unclear whether there is variation in HIV-1 env and gag between cerebrospinal fluid (CSF) and plasma of individuals with HIV-associated cryptococcal meningitis (CM). We compared HIV-1 env characteristics and the gag cytotoxic T-lymphocyte (CTL) escape mutations from CSF and plasma samples. Employing population-based Sanger sequencing, we sequenced HIV-1 env from CSF of 25 patients and plasma of 26 patients. For gag, 15 CSF and 21 plasma samples were successfully sequenced. Of these, 18 and 9 were paired env and gag CSF/plasma samples, respectively. There was no statistically significant difference in the proportion of CCR5-using strains in the CSF and plasma, (p = 0.50). Discordant CSF/plasma virus co-receptor use was found in 2/18 pairs (11.1%). The polymorphisms in the HIV-1 V3 loop were concordant between the two compartments. From the HIV-1 gag sequences, three pairs had discordant CTL escape mutations in three different epitopes of the nine analyzed. These findings suggest little variation in the HIV-1 env between plasma and CSF and that the CCR5-using strains predominate in both compartments. HIV-1 gag CTL escape mutations also displayed little variation in CSF and plasma suggesting similar CTL selective pressure. MDPI 2020-12-07 /pmc/articles/PMC7762280/ /pubmed/33297399 http://dx.doi.org/10.3390/v12121404 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kelentse, Nametso
Moyo, Sikhulile
Mogwele, Mompati L.
Ditshwanelo, Doreen
Mokaleng, Baitshepi
Moraka, Natasha O.
Lechiile, Kwana
Leeme, Tshepo B.
Lawrence, David S.
Musonda, Rosemary
Kasvosve, Ishmael
Harrison, Thomas S.
Jarvis, Joseph N.
Gaseitsiwe, Simani
HIV-1C env and gag Variation in the Cerebrospinal Fluid and Plasma of Patients with HIV-Associated Cryptococcal Meningitis in Botswana
title HIV-1C env and gag Variation in the Cerebrospinal Fluid and Plasma of Patients with HIV-Associated Cryptococcal Meningitis in Botswana
title_full HIV-1C env and gag Variation in the Cerebrospinal Fluid and Plasma of Patients with HIV-Associated Cryptococcal Meningitis in Botswana
title_fullStr HIV-1C env and gag Variation in the Cerebrospinal Fluid and Plasma of Patients with HIV-Associated Cryptococcal Meningitis in Botswana
title_full_unstemmed HIV-1C env and gag Variation in the Cerebrospinal Fluid and Plasma of Patients with HIV-Associated Cryptococcal Meningitis in Botswana
title_short HIV-1C env and gag Variation in the Cerebrospinal Fluid and Plasma of Patients with HIV-Associated Cryptococcal Meningitis in Botswana
title_sort hiv-1c env and gag variation in the cerebrospinal fluid and plasma of patients with hiv-associated cryptococcal meningitis in botswana
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7762280/
https://www.ncbi.nlm.nih.gov/pubmed/33297399
http://dx.doi.org/10.3390/v12121404
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