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Genetic Analysis and Molecular Mapping of the Quantitative Trait Loci Governing Low Phytic Acid Content in a Novel LPA Rice Mutant, PLM11
Breeding rice varieties with a low phytic acid (LPA) content is an effective strategy to overcome micronutrient deficiency in a population which consume rice as a staple food. An LPA mutant, Pusa LPA Mutant 11 (PLM11), was identified from an ethyl methane sulfonate (EMS)-induced population of Nagina...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7762397/ https://www.ncbi.nlm.nih.gov/pubmed/33302334 http://dx.doi.org/10.3390/plants9121728 |
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author | Gyani, Prem Chand Bollinedi, Haritha Gopala Krishnan, Subbaiyan Vinod, Kunnummal Kurungara Sachdeva, Archana Bhowmick, Prolay Kumar Ellur, Ranjith Kumar Nagarajan, Mariappan Singh, Ashok Kumar |
author_facet | Gyani, Prem Chand Bollinedi, Haritha Gopala Krishnan, Subbaiyan Vinod, Kunnummal Kurungara Sachdeva, Archana Bhowmick, Prolay Kumar Ellur, Ranjith Kumar Nagarajan, Mariappan Singh, Ashok Kumar |
author_sort | Gyani, Prem Chand |
collection | PubMed |
description | Breeding rice varieties with a low phytic acid (LPA) content is an effective strategy to overcome micronutrient deficiency in a population which consume rice as a staple food. An LPA mutant, Pusa LPA Mutant 11 (PLM11), was identified from an ethyl methane sulfonate (EMS)-induced population of Nagina 22. The present study was carried out to map the loci governing the LPA trait in PLM11 using an F(2:3) population derived from a cross between a high phytic acid rice variety, Pusa Basmati 6, with PLM11. The genotyping of the F(2) population with 78 polymorphic SSR markers followed by the estimation of phytic acid content in the seeds harvested from 176 F(2) plants helped in mapping a major QTL, qLPA8.1, explaining a 22.2% phenotypic variation on Chromosome 8. The QTL was delimited to a 1.96 cM region flanked by the markers RM25 and RM22832. Since there are no previous reports of a QTL/gene governing the LPA content in rice in this region, the QTL qLPA8.1 is a novel QTL. In silico analysis based on the annotated physical map of rice suggested the possible involvement of a locus, Os08g0274775, encoding for a protein similar to a phosphatidylinositol 3- and 4-kinase family member. This needs further validation and fine mapping. Since this QTL is currently specific to PLM11, the linked markers can be utilized for the development of rice varieties with reduced phytic acid (PA) content using PLM11 as the donor, thus enhancing the bioavailability of mineral micronutrients in humans. |
format | Online Article Text |
id | pubmed-7762397 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77623972020-12-26 Genetic Analysis and Molecular Mapping of the Quantitative Trait Loci Governing Low Phytic Acid Content in a Novel LPA Rice Mutant, PLM11 Gyani, Prem Chand Bollinedi, Haritha Gopala Krishnan, Subbaiyan Vinod, Kunnummal Kurungara Sachdeva, Archana Bhowmick, Prolay Kumar Ellur, Ranjith Kumar Nagarajan, Mariappan Singh, Ashok Kumar Plants (Basel) Article Breeding rice varieties with a low phytic acid (LPA) content is an effective strategy to overcome micronutrient deficiency in a population which consume rice as a staple food. An LPA mutant, Pusa LPA Mutant 11 (PLM11), was identified from an ethyl methane sulfonate (EMS)-induced population of Nagina 22. The present study was carried out to map the loci governing the LPA trait in PLM11 using an F(2:3) population derived from a cross between a high phytic acid rice variety, Pusa Basmati 6, with PLM11. The genotyping of the F(2) population with 78 polymorphic SSR markers followed by the estimation of phytic acid content in the seeds harvested from 176 F(2) plants helped in mapping a major QTL, qLPA8.1, explaining a 22.2% phenotypic variation on Chromosome 8. The QTL was delimited to a 1.96 cM region flanked by the markers RM25 and RM22832. Since there are no previous reports of a QTL/gene governing the LPA content in rice in this region, the QTL qLPA8.1 is a novel QTL. In silico analysis based on the annotated physical map of rice suggested the possible involvement of a locus, Os08g0274775, encoding for a protein similar to a phosphatidylinositol 3- and 4-kinase family member. This needs further validation and fine mapping. Since this QTL is currently specific to PLM11, the linked markers can be utilized for the development of rice varieties with reduced phytic acid (PA) content using PLM11 as the donor, thus enhancing the bioavailability of mineral micronutrients in humans. MDPI 2020-12-08 /pmc/articles/PMC7762397/ /pubmed/33302334 http://dx.doi.org/10.3390/plants9121728 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Gyani, Prem Chand Bollinedi, Haritha Gopala Krishnan, Subbaiyan Vinod, Kunnummal Kurungara Sachdeva, Archana Bhowmick, Prolay Kumar Ellur, Ranjith Kumar Nagarajan, Mariappan Singh, Ashok Kumar Genetic Analysis and Molecular Mapping of the Quantitative Trait Loci Governing Low Phytic Acid Content in a Novel LPA Rice Mutant, PLM11 |
title | Genetic Analysis and Molecular Mapping of the Quantitative Trait Loci Governing Low Phytic Acid Content in a Novel LPA Rice Mutant, PLM11 |
title_full | Genetic Analysis and Molecular Mapping of the Quantitative Trait Loci Governing Low Phytic Acid Content in a Novel LPA Rice Mutant, PLM11 |
title_fullStr | Genetic Analysis and Molecular Mapping of the Quantitative Trait Loci Governing Low Phytic Acid Content in a Novel LPA Rice Mutant, PLM11 |
title_full_unstemmed | Genetic Analysis and Molecular Mapping of the Quantitative Trait Loci Governing Low Phytic Acid Content in a Novel LPA Rice Mutant, PLM11 |
title_short | Genetic Analysis and Molecular Mapping of the Quantitative Trait Loci Governing Low Phytic Acid Content in a Novel LPA Rice Mutant, PLM11 |
title_sort | genetic analysis and molecular mapping of the quantitative trait loci governing low phytic acid content in a novel lpa rice mutant, plm11 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7762397/ https://www.ncbi.nlm.nih.gov/pubmed/33302334 http://dx.doi.org/10.3390/plants9121728 |
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