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Glucosylceramide Plays a Role in Fungal Germination, Lipid Raft Organization and Biofilm Adhesion of the Pathogenic Fungus Scedosporium aurantiacum

Infections caused by Scedosporium species present a wide range of clinical manifestations, from superficial to disseminated, especially in immunocompromised patients. Glucosylceramides (GlcCer) are glycosphingolipids found on the fungal cell surface and play an important role in growth and pathogeni...

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Autores principales: Rochetti, Victor Pereira, Rollin-Pinheiro, Rodrigo, de Oliveira, Evely Bertulino, Xisto, Mariana Ingrid Dutra da Silva, Barreto-Bergter, Eliana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7762401/
https://www.ncbi.nlm.nih.gov/pubmed/33302332
http://dx.doi.org/10.3390/jof6040345
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author Rochetti, Victor Pereira
Rollin-Pinheiro, Rodrigo
de Oliveira, Evely Bertulino
Xisto, Mariana Ingrid Dutra da Silva
Barreto-Bergter, Eliana
author_facet Rochetti, Victor Pereira
Rollin-Pinheiro, Rodrigo
de Oliveira, Evely Bertulino
Xisto, Mariana Ingrid Dutra da Silva
Barreto-Bergter, Eliana
author_sort Rochetti, Victor Pereira
collection PubMed
description Infections caused by Scedosporium species present a wide range of clinical manifestations, from superficial to disseminated, especially in immunocompromised patients. Glucosylceramides (GlcCer) are glycosphingolipids found on the fungal cell surface and play an important role in growth and pathogenicity processes in different fungi. The present study aimed to evaluate the structure of GlcCer and its role during growth in two S. aurantiacum isolates. Purified GlcCer from both isolates were obtained and its chemical structure identified by mass spectrometry. Using ELISA and immunofluorescence techniques it was observed that germination and NaOH-treatment of conidia favor GlcCer exposure. Monoclonal anti-GlcCer antibody reduced germination when cultivated with the inhibitor of melanin synthesis tricyclazole and also reduced germ tube length of conidia, both cultivated or not with tricyclazole. It was also demonstrated that anti-GlcCer altered lipid rafts organization, as shown by using the fluorescent stain filipin, but did not affect the susceptibility of the cell surface to damaging agents. Anti-GlcCer reduced total biomass and viability in biofilms formed on polystyrene plates. In the presence of anti-GlcCer, germinated S. aurantiacum conidia and biofilms could not adhere to polystyrene with the same efficacy as control cells. These results highlight the relevance of GlcCer in growth processes of S. aurantiacum.
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spelling pubmed-77624012020-12-26 Glucosylceramide Plays a Role in Fungal Germination, Lipid Raft Organization and Biofilm Adhesion of the Pathogenic Fungus Scedosporium aurantiacum Rochetti, Victor Pereira Rollin-Pinheiro, Rodrigo de Oliveira, Evely Bertulino Xisto, Mariana Ingrid Dutra da Silva Barreto-Bergter, Eliana J Fungi (Basel) Article Infections caused by Scedosporium species present a wide range of clinical manifestations, from superficial to disseminated, especially in immunocompromised patients. Glucosylceramides (GlcCer) are glycosphingolipids found on the fungal cell surface and play an important role in growth and pathogenicity processes in different fungi. The present study aimed to evaluate the structure of GlcCer and its role during growth in two S. aurantiacum isolates. Purified GlcCer from both isolates were obtained and its chemical structure identified by mass spectrometry. Using ELISA and immunofluorescence techniques it was observed that germination and NaOH-treatment of conidia favor GlcCer exposure. Monoclonal anti-GlcCer antibody reduced germination when cultivated with the inhibitor of melanin synthesis tricyclazole and also reduced germ tube length of conidia, both cultivated or not with tricyclazole. It was also demonstrated that anti-GlcCer altered lipid rafts organization, as shown by using the fluorescent stain filipin, but did not affect the susceptibility of the cell surface to damaging agents. Anti-GlcCer reduced total biomass and viability in biofilms formed on polystyrene plates. In the presence of anti-GlcCer, germinated S. aurantiacum conidia and biofilms could not adhere to polystyrene with the same efficacy as control cells. These results highlight the relevance of GlcCer in growth processes of S. aurantiacum. MDPI 2020-12-08 /pmc/articles/PMC7762401/ /pubmed/33302332 http://dx.doi.org/10.3390/jof6040345 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rochetti, Victor Pereira
Rollin-Pinheiro, Rodrigo
de Oliveira, Evely Bertulino
Xisto, Mariana Ingrid Dutra da Silva
Barreto-Bergter, Eliana
Glucosylceramide Plays a Role in Fungal Germination, Lipid Raft Organization and Biofilm Adhesion of the Pathogenic Fungus Scedosporium aurantiacum
title Glucosylceramide Plays a Role in Fungal Germination, Lipid Raft Organization and Biofilm Adhesion of the Pathogenic Fungus Scedosporium aurantiacum
title_full Glucosylceramide Plays a Role in Fungal Germination, Lipid Raft Organization and Biofilm Adhesion of the Pathogenic Fungus Scedosporium aurantiacum
title_fullStr Glucosylceramide Plays a Role in Fungal Germination, Lipid Raft Organization and Biofilm Adhesion of the Pathogenic Fungus Scedosporium aurantiacum
title_full_unstemmed Glucosylceramide Plays a Role in Fungal Germination, Lipid Raft Organization and Biofilm Adhesion of the Pathogenic Fungus Scedosporium aurantiacum
title_short Glucosylceramide Plays a Role in Fungal Germination, Lipid Raft Organization and Biofilm Adhesion of the Pathogenic Fungus Scedosporium aurantiacum
title_sort glucosylceramide plays a role in fungal germination, lipid raft organization and biofilm adhesion of the pathogenic fungus scedosporium aurantiacum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7762401/
https://www.ncbi.nlm.nih.gov/pubmed/33302332
http://dx.doi.org/10.3390/jof6040345
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