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Sensitive and In Situ Hemoglobin Detection Based on a Graphene Oxide Functionalized Microfiber
The determination of hemoglobin (Hb) level is indispensable in the pathological study of many blood diseases. Graphene oxide (GO), with its excellent optical properties and great biocompatibility, has attracted significant attention and been widely utilized in biochemical detection. Here, we report...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7763212/ https://www.ncbi.nlm.nih.gov/pubmed/33317010 http://dx.doi.org/10.3390/nano10122461 |
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author | Fang, Fang Li, Yanpeng Yang, Liuyang Li, Liangye Yan, Zhijun Sun, Qizhen |
author_facet | Fang, Fang Li, Yanpeng Yang, Liuyang Li, Liangye Yan, Zhijun Sun, Qizhen |
author_sort | Fang, Fang |
collection | PubMed |
description | The determination of hemoglobin (Hb) level is indispensable in the pathological study of many blood diseases. Graphene oxide (GO), with its excellent optical properties and great biocompatibility, has attracted significant attention and been widely utilized in biochemical detection. Here, we report an ultrasensitive Hb sensor based on a graphene oxide (GO)-coated microfiber. The GO was utilized as a linking layer deposited on the microfiber surface, which can provide an enhanced local evanescent light field and abundant bonding sites for Hb molecules. The optical microfiber with a compact structure and a strong evanescent light field served as the platform for biosensing. The surface morphology characterized by optical microscope, scanning electron microscope, and Raman spectroscopy offers detailed evidence for the success of GO deposition. The dynamic bonding between GO and target Hb molecules was monitored in real-time through an optical spectrum analyzer. An ultrahigh sensitivity of 6.02 nm/(mg/mL) with a detection limit of 0.17 μg/mL was achieved by tracking the resonant wavelength shift of spectra. It is important to highlight that the detection limit of GO-coated microfiber is 1–2 orders of magnitude lower than other reported fiber optic Hb sensors. Benefiting from high sensitivity, low cost, small size, and fast response, the proposed sensing microfiber coated with GO could be a competitive alternative in the diagnosis of blood diseases and a subject of further research in the medical field. |
format | Online Article Text |
id | pubmed-7763212 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77632122020-12-27 Sensitive and In Situ Hemoglobin Detection Based on a Graphene Oxide Functionalized Microfiber Fang, Fang Li, Yanpeng Yang, Liuyang Li, Liangye Yan, Zhijun Sun, Qizhen Nanomaterials (Basel) Article The determination of hemoglobin (Hb) level is indispensable in the pathological study of many blood diseases. Graphene oxide (GO), with its excellent optical properties and great biocompatibility, has attracted significant attention and been widely utilized in biochemical detection. Here, we report an ultrasensitive Hb sensor based on a graphene oxide (GO)-coated microfiber. The GO was utilized as a linking layer deposited on the microfiber surface, which can provide an enhanced local evanescent light field and abundant bonding sites for Hb molecules. The optical microfiber with a compact structure and a strong evanescent light field served as the platform for biosensing. The surface morphology characterized by optical microscope, scanning electron microscope, and Raman spectroscopy offers detailed evidence for the success of GO deposition. The dynamic bonding between GO and target Hb molecules was monitored in real-time through an optical spectrum analyzer. An ultrahigh sensitivity of 6.02 nm/(mg/mL) with a detection limit of 0.17 μg/mL was achieved by tracking the resonant wavelength shift of spectra. It is important to highlight that the detection limit of GO-coated microfiber is 1–2 orders of magnitude lower than other reported fiber optic Hb sensors. Benefiting from high sensitivity, low cost, small size, and fast response, the proposed sensing microfiber coated with GO could be a competitive alternative in the diagnosis of blood diseases and a subject of further research in the medical field. MDPI 2020-12-09 /pmc/articles/PMC7763212/ /pubmed/33317010 http://dx.doi.org/10.3390/nano10122461 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Fang, Fang Li, Yanpeng Yang, Liuyang Li, Liangye Yan, Zhijun Sun, Qizhen Sensitive and In Situ Hemoglobin Detection Based on a Graphene Oxide Functionalized Microfiber |
title | Sensitive and In Situ Hemoglobin Detection Based on a Graphene Oxide Functionalized Microfiber |
title_full | Sensitive and In Situ Hemoglobin Detection Based on a Graphene Oxide Functionalized Microfiber |
title_fullStr | Sensitive and In Situ Hemoglobin Detection Based on a Graphene Oxide Functionalized Microfiber |
title_full_unstemmed | Sensitive and In Situ Hemoglobin Detection Based on a Graphene Oxide Functionalized Microfiber |
title_short | Sensitive and In Situ Hemoglobin Detection Based on a Graphene Oxide Functionalized Microfiber |
title_sort | sensitive and in situ hemoglobin detection based on a graphene oxide functionalized microfiber |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7763212/ https://www.ncbi.nlm.nih.gov/pubmed/33317010 http://dx.doi.org/10.3390/nano10122461 |
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