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Cell Sheets Restore Secretory Function in Wounded Mouse Submandibular Glands
Thermoresponsive cell culture plates release cells as confluent living sheets in response to small changes in temperature, with recovered cell sheets retaining functional extracellular matrix proteins and tight junctions, both of which indicate formation of intact and functional tissue. Our recent s...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7763220/ https://www.ncbi.nlm.nih.gov/pubmed/33316992 http://dx.doi.org/10.3390/cells9122645 |
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author | dos Santos, Harim T. Kim, Kyungsook Okano, Teruo Camden, Jean M. Weisman, Gary A. Baker, Olga J. Nam, Kihoon |
author_facet | dos Santos, Harim T. Kim, Kyungsook Okano, Teruo Camden, Jean M. Weisman, Gary A. Baker, Olga J. Nam, Kihoon |
author_sort | dos Santos, Harim T. |
collection | PubMed |
description | Thermoresponsive cell culture plates release cells as confluent living sheets in response to small changes in temperature, with recovered cell sheets retaining functional extracellular matrix proteins and tight junctions, both of which indicate formation of intact and functional tissue. Our recent studies demonstrated that cell sheets are highly effective in promoting mouse submandibular gland (SMG) cell differentiation and recovering tissue integrity. However, these studies were performed only at early time points and extension of the observation period is needed to investigate duration of the cell sheets. Thus, the goal of this study was to demonstrate that treatment of wounded mouse SMG with cell sheets is capable of increasing salivary epithelial integrity over extended time periods. The results indicate that cell sheets promote tissue organization as early as eight days after transplantation and that these effects endure through Day 20. Furthermore, cell sheet transplantation in wounded SMG induces a significant time-dependent enhancement of cell polarization, differentiation and ion transporter expression. Finally, this treatment restored saliva quantity to pre-wounding levels at both eight and twenty days post-surgery and significantly improved saliva quality at twenty days post-surgery. These data indicate that cell sheets engineered with thermoresponsive cell culture plates are useful for salivary gland regeneration and provide evidence for the long-term stability of cell sheets, thereby offering a potential new therapeutic strategy for treating hyposalivation. |
format | Online Article Text |
id | pubmed-7763220 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77632202020-12-27 Cell Sheets Restore Secretory Function in Wounded Mouse Submandibular Glands dos Santos, Harim T. Kim, Kyungsook Okano, Teruo Camden, Jean M. Weisman, Gary A. Baker, Olga J. Nam, Kihoon Cells Article Thermoresponsive cell culture plates release cells as confluent living sheets in response to small changes in temperature, with recovered cell sheets retaining functional extracellular matrix proteins and tight junctions, both of which indicate formation of intact and functional tissue. Our recent studies demonstrated that cell sheets are highly effective in promoting mouse submandibular gland (SMG) cell differentiation and recovering tissue integrity. However, these studies were performed only at early time points and extension of the observation period is needed to investigate duration of the cell sheets. Thus, the goal of this study was to demonstrate that treatment of wounded mouse SMG with cell sheets is capable of increasing salivary epithelial integrity over extended time periods. The results indicate that cell sheets promote tissue organization as early as eight days after transplantation and that these effects endure through Day 20. Furthermore, cell sheet transplantation in wounded SMG induces a significant time-dependent enhancement of cell polarization, differentiation and ion transporter expression. Finally, this treatment restored saliva quantity to pre-wounding levels at both eight and twenty days post-surgery and significantly improved saliva quality at twenty days post-surgery. These data indicate that cell sheets engineered with thermoresponsive cell culture plates are useful for salivary gland regeneration and provide evidence for the long-term stability of cell sheets, thereby offering a potential new therapeutic strategy for treating hyposalivation. MDPI 2020-12-09 /pmc/articles/PMC7763220/ /pubmed/33316992 http://dx.doi.org/10.3390/cells9122645 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article dos Santos, Harim T. Kim, Kyungsook Okano, Teruo Camden, Jean M. Weisman, Gary A. Baker, Olga J. Nam, Kihoon Cell Sheets Restore Secretory Function in Wounded Mouse Submandibular Glands |
title | Cell Sheets Restore Secretory Function in Wounded Mouse Submandibular Glands |
title_full | Cell Sheets Restore Secretory Function in Wounded Mouse Submandibular Glands |
title_fullStr | Cell Sheets Restore Secretory Function in Wounded Mouse Submandibular Glands |
title_full_unstemmed | Cell Sheets Restore Secretory Function in Wounded Mouse Submandibular Glands |
title_short | Cell Sheets Restore Secretory Function in Wounded Mouse Submandibular Glands |
title_sort | cell sheets restore secretory function in wounded mouse submandibular glands |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7763220/ https://www.ncbi.nlm.nih.gov/pubmed/33316992 http://dx.doi.org/10.3390/cells9122645 |
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