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Therapeutic Effects of Gallic Acid in Regulating Senescence and Diabetes; an In Vitro Study
Gallic acid (GA), a plant-derived ubiquitous secondary polyphenol metabolite, can be a useful dietary supplement. This in vitro study’s primary purpose was to assess the anti-aging properties of GA using rat embryonic fibroblast (REF) cells, antidiabetic effects via pancreatic islet cells, and final...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7763304/ https://www.ncbi.nlm.nih.gov/pubmed/33322612 http://dx.doi.org/10.3390/molecules25245875 |
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author | Rahimifard, Mahban Baeeri, Maryam Bahadar, Haji Moini-Nodeh, Shermineh Khalid, Madiha Haghi-Aminjan, Hamed Mohammadian, Hossein Abdollahi, Mohammad |
author_facet | Rahimifard, Mahban Baeeri, Maryam Bahadar, Haji Moini-Nodeh, Shermineh Khalid, Madiha Haghi-Aminjan, Hamed Mohammadian, Hossein Abdollahi, Mohammad |
author_sort | Rahimifard, Mahban |
collection | PubMed |
description | Gallic acid (GA), a plant-derived ubiquitous secondary polyphenol metabolite, can be a useful dietary supplement. This in vitro study’s primary purpose was to assess the anti-aging properties of GA using rat embryonic fibroblast (REF) cells, antidiabetic effects via pancreatic islet cells, and finally, elucidating the molecular mechanisms of this natural compound. REF and islet cells were isolated from fetuses and pancreas of rats, respectively. Then, several senescence-associated molecular and biochemical parameters, along with antidiabetic markers, were investigated. GA caused a significant decrease in the β-galactosidase activity and reduced inflammatory cytokines and oxidative stress markers in REF cells. GA reduced the G0/G1 phase in senescent REF cells that led cells to G2/M. Besides, GA improved the function of the β cells. Flow cytometry and spectrophotometric analysis showed that it reduces apoptosis via inhibiting caspase-9 activity. Taken together, based on the present findings, this polyphenol metabolite at low doses regulates different pathways of senescence and diabetes through its antioxidative stress potential and modulation of mitochondrial complexes activities. |
format | Online Article Text |
id | pubmed-7763304 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77633042020-12-27 Therapeutic Effects of Gallic Acid in Regulating Senescence and Diabetes; an In Vitro Study Rahimifard, Mahban Baeeri, Maryam Bahadar, Haji Moini-Nodeh, Shermineh Khalid, Madiha Haghi-Aminjan, Hamed Mohammadian, Hossein Abdollahi, Mohammad Molecules Article Gallic acid (GA), a plant-derived ubiquitous secondary polyphenol metabolite, can be a useful dietary supplement. This in vitro study’s primary purpose was to assess the anti-aging properties of GA using rat embryonic fibroblast (REF) cells, antidiabetic effects via pancreatic islet cells, and finally, elucidating the molecular mechanisms of this natural compound. REF and islet cells were isolated from fetuses and pancreas of rats, respectively. Then, several senescence-associated molecular and biochemical parameters, along with antidiabetic markers, were investigated. GA caused a significant decrease in the β-galactosidase activity and reduced inflammatory cytokines and oxidative stress markers in REF cells. GA reduced the G0/G1 phase in senescent REF cells that led cells to G2/M. Besides, GA improved the function of the β cells. Flow cytometry and spectrophotometric analysis showed that it reduces apoptosis via inhibiting caspase-9 activity. Taken together, based on the present findings, this polyphenol metabolite at low doses regulates different pathways of senescence and diabetes through its antioxidative stress potential and modulation of mitochondrial complexes activities. MDPI 2020-12-11 /pmc/articles/PMC7763304/ /pubmed/33322612 http://dx.doi.org/10.3390/molecules25245875 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Rahimifard, Mahban Baeeri, Maryam Bahadar, Haji Moini-Nodeh, Shermineh Khalid, Madiha Haghi-Aminjan, Hamed Mohammadian, Hossein Abdollahi, Mohammad Therapeutic Effects of Gallic Acid in Regulating Senescence and Diabetes; an In Vitro Study |
title | Therapeutic Effects of Gallic Acid in Regulating Senescence and Diabetes; an In Vitro Study |
title_full | Therapeutic Effects of Gallic Acid in Regulating Senescence and Diabetes; an In Vitro Study |
title_fullStr | Therapeutic Effects of Gallic Acid in Regulating Senescence and Diabetes; an In Vitro Study |
title_full_unstemmed | Therapeutic Effects of Gallic Acid in Regulating Senescence and Diabetes; an In Vitro Study |
title_short | Therapeutic Effects of Gallic Acid in Regulating Senescence and Diabetes; an In Vitro Study |
title_sort | therapeutic effects of gallic acid in regulating senescence and diabetes; an in vitro study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7763304/ https://www.ncbi.nlm.nih.gov/pubmed/33322612 http://dx.doi.org/10.3390/molecules25245875 |
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