Cargando…

Transcriptome Analyses Reveal Differential Transcriptional Profiles in Early- and Late-Dividing Porcine Somatic Cell Nuclear Transfer Embryos

Somatic cell nuclear transfer (SCNT) is not only a valuable tool for understanding nuclear reprogramming, but it also facilitates the generation of genetically modified animals. However, the development of SCNT embryos has remained an uncontrollable process. It was reported that the SCNT embryos tha...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Zhiguo, Xiang, Guangming, Xu, Kui, Che, Jingjing, Xu, Changjiang, Li, Kui, Wang, Bingyuan, Mu, Yulian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7763450/
https://www.ncbi.nlm.nih.gov/pubmed/33322792
http://dx.doi.org/10.3390/genes11121499
_version_ 1783628021550284800
author Liu, Zhiguo
Xiang, Guangming
Xu, Kui
Che, Jingjing
Xu, Changjiang
Li, Kui
Wang, Bingyuan
Mu, Yulian
author_facet Liu, Zhiguo
Xiang, Guangming
Xu, Kui
Che, Jingjing
Xu, Changjiang
Li, Kui
Wang, Bingyuan
Mu, Yulian
author_sort Liu, Zhiguo
collection PubMed
description Somatic cell nuclear transfer (SCNT) is not only a valuable tool for understanding nuclear reprogramming, but it also facilitates the generation of genetically modified animals. However, the development of SCNT embryos has remained an uncontrollable process. It was reported that the SCNT embryos that complete the first cell division sooner are more likely to develop to the blastocyst stage, suggesting their better developmental competence. Therefore, to better understand the underlying molecular mechanisms, RNA-seq of pig SCNT embryos that were early-dividing (24 h postactivation) and late-dividing (36 h postactivation) was performed. Our analysis revealed that early- and late-dividing embryos have distinct RNA profiles, and, in all, 3077 genes were differentially expressed. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses revealed that early-dividing embryos exhibited higher expression in genes that participated in the meiotic cell cycle, while enrichment of RNA processing- and translation-related genes was found in late-dividing embryos. There are also fewer somatic memory genes such as FLRT2, ADAMTS1, and FOXR1, which are abnormally activated or suppressed in early-dividing cloned embryos. These results show that early-dividing SCNT embryos have different transcriptional profiles than late-dividing embryos. Early division of SCNT embryos may be associated with their better reprogramming capacity, and somatic memory genes may act as a reprogramming barrier in pig SCNT reprogramming.
format Online
Article
Text
id pubmed-7763450
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-77634502020-12-27 Transcriptome Analyses Reveal Differential Transcriptional Profiles in Early- and Late-Dividing Porcine Somatic Cell Nuclear Transfer Embryos Liu, Zhiguo Xiang, Guangming Xu, Kui Che, Jingjing Xu, Changjiang Li, Kui Wang, Bingyuan Mu, Yulian Genes (Basel) Article Somatic cell nuclear transfer (SCNT) is not only a valuable tool for understanding nuclear reprogramming, but it also facilitates the generation of genetically modified animals. However, the development of SCNT embryos has remained an uncontrollable process. It was reported that the SCNT embryos that complete the first cell division sooner are more likely to develop to the blastocyst stage, suggesting their better developmental competence. Therefore, to better understand the underlying molecular mechanisms, RNA-seq of pig SCNT embryos that were early-dividing (24 h postactivation) and late-dividing (36 h postactivation) was performed. Our analysis revealed that early- and late-dividing embryos have distinct RNA profiles, and, in all, 3077 genes were differentially expressed. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses revealed that early-dividing embryos exhibited higher expression in genes that participated in the meiotic cell cycle, while enrichment of RNA processing- and translation-related genes was found in late-dividing embryos. There are also fewer somatic memory genes such as FLRT2, ADAMTS1, and FOXR1, which are abnormally activated or suppressed in early-dividing cloned embryos. These results show that early-dividing SCNT embryos have different transcriptional profiles than late-dividing embryos. Early division of SCNT embryos may be associated with their better reprogramming capacity, and somatic memory genes may act as a reprogramming barrier in pig SCNT reprogramming. MDPI 2020-12-12 /pmc/articles/PMC7763450/ /pubmed/33322792 http://dx.doi.org/10.3390/genes11121499 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Zhiguo
Xiang, Guangming
Xu, Kui
Che, Jingjing
Xu, Changjiang
Li, Kui
Wang, Bingyuan
Mu, Yulian
Transcriptome Analyses Reveal Differential Transcriptional Profiles in Early- and Late-Dividing Porcine Somatic Cell Nuclear Transfer Embryos
title Transcriptome Analyses Reveal Differential Transcriptional Profiles in Early- and Late-Dividing Porcine Somatic Cell Nuclear Transfer Embryos
title_full Transcriptome Analyses Reveal Differential Transcriptional Profiles in Early- and Late-Dividing Porcine Somatic Cell Nuclear Transfer Embryos
title_fullStr Transcriptome Analyses Reveal Differential Transcriptional Profiles in Early- and Late-Dividing Porcine Somatic Cell Nuclear Transfer Embryos
title_full_unstemmed Transcriptome Analyses Reveal Differential Transcriptional Profiles in Early- and Late-Dividing Porcine Somatic Cell Nuclear Transfer Embryos
title_short Transcriptome Analyses Reveal Differential Transcriptional Profiles in Early- and Late-Dividing Porcine Somatic Cell Nuclear Transfer Embryos
title_sort transcriptome analyses reveal differential transcriptional profiles in early- and late-dividing porcine somatic cell nuclear transfer embryos
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7763450/
https://www.ncbi.nlm.nih.gov/pubmed/33322792
http://dx.doi.org/10.3390/genes11121499
work_keys_str_mv AT liuzhiguo transcriptomeanalysesrevealdifferentialtranscriptionalprofilesinearlyandlatedividingporcinesomaticcellnucleartransferembryos
AT xiangguangming transcriptomeanalysesrevealdifferentialtranscriptionalprofilesinearlyandlatedividingporcinesomaticcellnucleartransferembryos
AT xukui transcriptomeanalysesrevealdifferentialtranscriptionalprofilesinearlyandlatedividingporcinesomaticcellnucleartransferembryos
AT chejingjing transcriptomeanalysesrevealdifferentialtranscriptionalprofilesinearlyandlatedividingporcinesomaticcellnucleartransferembryos
AT xuchangjiang transcriptomeanalysesrevealdifferentialtranscriptionalprofilesinearlyandlatedividingporcinesomaticcellnucleartransferembryos
AT likui transcriptomeanalysesrevealdifferentialtranscriptionalprofilesinearlyandlatedividingporcinesomaticcellnucleartransferembryos
AT wangbingyuan transcriptomeanalysesrevealdifferentialtranscriptionalprofilesinearlyandlatedividingporcinesomaticcellnucleartransferembryos
AT muyulian transcriptomeanalysesrevealdifferentialtranscriptionalprofilesinearlyandlatedividingporcinesomaticcellnucleartransferembryos