Cargando…
The Collagen-Based Medical Device MD-Tissue Acts as a Mechanical Scaffold Influencing Morpho-Functional Properties of Cultured Human Tenocytes
Mechanotransduction is the ability of cells to translate mechanical stimuli into biochemical signals that can ultimately influence gene expression, cell morphology and cell fate. Tenocytes are responsible for tendon mechanical adaptation converting mechanical stimuli imposed during mechanical loadin...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7763591/ https://www.ncbi.nlm.nih.gov/pubmed/33302563 http://dx.doi.org/10.3390/cells9122641 |
_version_ | 1783628054515417088 |
---|---|
author | Randelli, Filippo Sartori, Patrizia Carlomagno, Cristiano Bedoni, Marzia Menon, Alessandra Vezzoli, Elena Sommariva, Michele Gagliano, Nicoletta |
author_facet | Randelli, Filippo Sartori, Patrizia Carlomagno, Cristiano Bedoni, Marzia Menon, Alessandra Vezzoli, Elena Sommariva, Michele Gagliano, Nicoletta |
author_sort | Randelli, Filippo |
collection | PubMed |
description | Mechanotransduction is the ability of cells to translate mechanical stimuli into biochemical signals that can ultimately influence gene expression, cell morphology and cell fate. Tenocytes are responsible for tendon mechanical adaptation converting mechanical stimuli imposed during mechanical loading, thus affecting extracellular matrix homeostasis. Since we previously demonstrated that MD-Tissue, an injectable collagen-based medical compound containing swine-derived collagen as the main component, is able to affect tenocyte properties, the aim of this study was to analyze whether the effects triggered by MD-Tissue were based on mechanotransduction-related mechanisms. For this purpose, MD-Tissue was used to coat Petri dishes and cytochalasin B was used to deprive tenocytes of mechanical stimulation mediated by the actin cytoskeleton. Cell morphology, migration, collagen turnover pathways and the expression of key mechanosensors were analyzed by morphological and molecular methods. Our findings confirm that MD-Tissue affects collagen turnover pathways and favors cell migration and show that the MD-Tissue-induced effect represents a mechanical input involving the mechanotransduction machinery. Overall, MD-Tissue, acting as a mechanical scaffold, could represent an effective medical device for a novel therapeutic, regenerative and rehabilitative approach to favor tendon healing in tendinopathies. |
format | Online Article Text |
id | pubmed-7763591 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77635912020-12-27 The Collagen-Based Medical Device MD-Tissue Acts as a Mechanical Scaffold Influencing Morpho-Functional Properties of Cultured Human Tenocytes Randelli, Filippo Sartori, Patrizia Carlomagno, Cristiano Bedoni, Marzia Menon, Alessandra Vezzoli, Elena Sommariva, Michele Gagliano, Nicoletta Cells Article Mechanotransduction is the ability of cells to translate mechanical stimuli into biochemical signals that can ultimately influence gene expression, cell morphology and cell fate. Tenocytes are responsible for tendon mechanical adaptation converting mechanical stimuli imposed during mechanical loading, thus affecting extracellular matrix homeostasis. Since we previously demonstrated that MD-Tissue, an injectable collagen-based medical compound containing swine-derived collagen as the main component, is able to affect tenocyte properties, the aim of this study was to analyze whether the effects triggered by MD-Tissue were based on mechanotransduction-related mechanisms. For this purpose, MD-Tissue was used to coat Petri dishes and cytochalasin B was used to deprive tenocytes of mechanical stimulation mediated by the actin cytoskeleton. Cell morphology, migration, collagen turnover pathways and the expression of key mechanosensors were analyzed by morphological and molecular methods. Our findings confirm that MD-Tissue affects collagen turnover pathways and favors cell migration and show that the MD-Tissue-induced effect represents a mechanical input involving the mechanotransduction machinery. Overall, MD-Tissue, acting as a mechanical scaffold, could represent an effective medical device for a novel therapeutic, regenerative and rehabilitative approach to favor tendon healing in tendinopathies. MDPI 2020-12-08 /pmc/articles/PMC7763591/ /pubmed/33302563 http://dx.doi.org/10.3390/cells9122641 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Randelli, Filippo Sartori, Patrizia Carlomagno, Cristiano Bedoni, Marzia Menon, Alessandra Vezzoli, Elena Sommariva, Michele Gagliano, Nicoletta The Collagen-Based Medical Device MD-Tissue Acts as a Mechanical Scaffold Influencing Morpho-Functional Properties of Cultured Human Tenocytes |
title | The Collagen-Based Medical Device MD-Tissue Acts as a Mechanical Scaffold Influencing Morpho-Functional Properties of Cultured Human Tenocytes |
title_full | The Collagen-Based Medical Device MD-Tissue Acts as a Mechanical Scaffold Influencing Morpho-Functional Properties of Cultured Human Tenocytes |
title_fullStr | The Collagen-Based Medical Device MD-Tissue Acts as a Mechanical Scaffold Influencing Morpho-Functional Properties of Cultured Human Tenocytes |
title_full_unstemmed | The Collagen-Based Medical Device MD-Tissue Acts as a Mechanical Scaffold Influencing Morpho-Functional Properties of Cultured Human Tenocytes |
title_short | The Collagen-Based Medical Device MD-Tissue Acts as a Mechanical Scaffold Influencing Morpho-Functional Properties of Cultured Human Tenocytes |
title_sort | collagen-based medical device md-tissue acts as a mechanical scaffold influencing morpho-functional properties of cultured human tenocytes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7763591/ https://www.ncbi.nlm.nih.gov/pubmed/33302563 http://dx.doi.org/10.3390/cells9122641 |
work_keys_str_mv | AT randellifilippo thecollagenbasedmedicaldevicemdtissueactsasamechanicalscaffoldinfluencingmorphofunctionalpropertiesofculturedhumantenocytes AT sartoripatrizia thecollagenbasedmedicaldevicemdtissueactsasamechanicalscaffoldinfluencingmorphofunctionalpropertiesofculturedhumantenocytes AT carlomagnocristiano thecollagenbasedmedicaldevicemdtissueactsasamechanicalscaffoldinfluencingmorphofunctionalpropertiesofculturedhumantenocytes AT bedonimarzia thecollagenbasedmedicaldevicemdtissueactsasamechanicalscaffoldinfluencingmorphofunctionalpropertiesofculturedhumantenocytes AT menonalessandra thecollagenbasedmedicaldevicemdtissueactsasamechanicalscaffoldinfluencingmorphofunctionalpropertiesofculturedhumantenocytes AT vezzolielena thecollagenbasedmedicaldevicemdtissueactsasamechanicalscaffoldinfluencingmorphofunctionalpropertiesofculturedhumantenocytes AT sommarivamichele thecollagenbasedmedicaldevicemdtissueactsasamechanicalscaffoldinfluencingmorphofunctionalpropertiesofculturedhumantenocytes AT gaglianonicoletta thecollagenbasedmedicaldevicemdtissueactsasamechanicalscaffoldinfluencingmorphofunctionalpropertiesofculturedhumantenocytes AT randellifilippo collagenbasedmedicaldevicemdtissueactsasamechanicalscaffoldinfluencingmorphofunctionalpropertiesofculturedhumantenocytes AT sartoripatrizia collagenbasedmedicaldevicemdtissueactsasamechanicalscaffoldinfluencingmorphofunctionalpropertiesofculturedhumantenocytes AT carlomagnocristiano collagenbasedmedicaldevicemdtissueactsasamechanicalscaffoldinfluencingmorphofunctionalpropertiesofculturedhumantenocytes AT bedonimarzia collagenbasedmedicaldevicemdtissueactsasamechanicalscaffoldinfluencingmorphofunctionalpropertiesofculturedhumantenocytes AT menonalessandra collagenbasedmedicaldevicemdtissueactsasamechanicalscaffoldinfluencingmorphofunctionalpropertiesofculturedhumantenocytes AT vezzolielena collagenbasedmedicaldevicemdtissueactsasamechanicalscaffoldinfluencingmorphofunctionalpropertiesofculturedhumantenocytes AT sommarivamichele collagenbasedmedicaldevicemdtissueactsasamechanicalscaffoldinfluencingmorphofunctionalpropertiesofculturedhumantenocytes AT gaglianonicoletta collagenbasedmedicaldevicemdtissueactsasamechanicalscaffoldinfluencingmorphofunctionalpropertiesofculturedhumantenocytes |