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The Collagen-Based Medical Device MD-Tissue Acts as a Mechanical Scaffold Influencing Morpho-Functional Properties of Cultured Human Tenocytes

Mechanotransduction is the ability of cells to translate mechanical stimuli into biochemical signals that can ultimately influence gene expression, cell morphology and cell fate. Tenocytes are responsible for tendon mechanical adaptation converting mechanical stimuli imposed during mechanical loadin...

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Autores principales: Randelli, Filippo, Sartori, Patrizia, Carlomagno, Cristiano, Bedoni, Marzia, Menon, Alessandra, Vezzoli, Elena, Sommariva, Michele, Gagliano, Nicoletta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7763591/
https://www.ncbi.nlm.nih.gov/pubmed/33302563
http://dx.doi.org/10.3390/cells9122641
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author Randelli, Filippo
Sartori, Patrizia
Carlomagno, Cristiano
Bedoni, Marzia
Menon, Alessandra
Vezzoli, Elena
Sommariva, Michele
Gagliano, Nicoletta
author_facet Randelli, Filippo
Sartori, Patrizia
Carlomagno, Cristiano
Bedoni, Marzia
Menon, Alessandra
Vezzoli, Elena
Sommariva, Michele
Gagliano, Nicoletta
author_sort Randelli, Filippo
collection PubMed
description Mechanotransduction is the ability of cells to translate mechanical stimuli into biochemical signals that can ultimately influence gene expression, cell morphology and cell fate. Tenocytes are responsible for tendon mechanical adaptation converting mechanical stimuli imposed during mechanical loading, thus affecting extracellular matrix homeostasis. Since we previously demonstrated that MD-Tissue, an injectable collagen-based medical compound containing swine-derived collagen as the main component, is able to affect tenocyte properties, the aim of this study was to analyze whether the effects triggered by MD-Tissue were based on mechanotransduction-related mechanisms. For this purpose, MD-Tissue was used to coat Petri dishes and cytochalasin B was used to deprive tenocytes of mechanical stimulation mediated by the actin cytoskeleton. Cell morphology, migration, collagen turnover pathways and the expression of key mechanosensors were analyzed by morphological and molecular methods. Our findings confirm that MD-Tissue affects collagen turnover pathways and favors cell migration and show that the MD-Tissue-induced effect represents a mechanical input involving the mechanotransduction machinery. Overall, MD-Tissue, acting as a mechanical scaffold, could represent an effective medical device for a novel therapeutic, regenerative and rehabilitative approach to favor tendon healing in tendinopathies.
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spelling pubmed-77635912020-12-27 The Collagen-Based Medical Device MD-Tissue Acts as a Mechanical Scaffold Influencing Morpho-Functional Properties of Cultured Human Tenocytes Randelli, Filippo Sartori, Patrizia Carlomagno, Cristiano Bedoni, Marzia Menon, Alessandra Vezzoli, Elena Sommariva, Michele Gagliano, Nicoletta Cells Article Mechanotransduction is the ability of cells to translate mechanical stimuli into biochemical signals that can ultimately influence gene expression, cell morphology and cell fate. Tenocytes are responsible for tendon mechanical adaptation converting mechanical stimuli imposed during mechanical loading, thus affecting extracellular matrix homeostasis. Since we previously demonstrated that MD-Tissue, an injectable collagen-based medical compound containing swine-derived collagen as the main component, is able to affect tenocyte properties, the aim of this study was to analyze whether the effects triggered by MD-Tissue were based on mechanotransduction-related mechanisms. For this purpose, MD-Tissue was used to coat Petri dishes and cytochalasin B was used to deprive tenocytes of mechanical stimulation mediated by the actin cytoskeleton. Cell morphology, migration, collagen turnover pathways and the expression of key mechanosensors were analyzed by morphological and molecular methods. Our findings confirm that MD-Tissue affects collagen turnover pathways and favors cell migration and show that the MD-Tissue-induced effect represents a mechanical input involving the mechanotransduction machinery. Overall, MD-Tissue, acting as a mechanical scaffold, could represent an effective medical device for a novel therapeutic, regenerative and rehabilitative approach to favor tendon healing in tendinopathies. MDPI 2020-12-08 /pmc/articles/PMC7763591/ /pubmed/33302563 http://dx.doi.org/10.3390/cells9122641 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Randelli, Filippo
Sartori, Patrizia
Carlomagno, Cristiano
Bedoni, Marzia
Menon, Alessandra
Vezzoli, Elena
Sommariva, Michele
Gagliano, Nicoletta
The Collagen-Based Medical Device MD-Tissue Acts as a Mechanical Scaffold Influencing Morpho-Functional Properties of Cultured Human Tenocytes
title The Collagen-Based Medical Device MD-Tissue Acts as a Mechanical Scaffold Influencing Morpho-Functional Properties of Cultured Human Tenocytes
title_full The Collagen-Based Medical Device MD-Tissue Acts as a Mechanical Scaffold Influencing Morpho-Functional Properties of Cultured Human Tenocytes
title_fullStr The Collagen-Based Medical Device MD-Tissue Acts as a Mechanical Scaffold Influencing Morpho-Functional Properties of Cultured Human Tenocytes
title_full_unstemmed The Collagen-Based Medical Device MD-Tissue Acts as a Mechanical Scaffold Influencing Morpho-Functional Properties of Cultured Human Tenocytes
title_short The Collagen-Based Medical Device MD-Tissue Acts as a Mechanical Scaffold Influencing Morpho-Functional Properties of Cultured Human Tenocytes
title_sort collagen-based medical device md-tissue acts as a mechanical scaffold influencing morpho-functional properties of cultured human tenocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7763591/
https://www.ncbi.nlm.nih.gov/pubmed/33302563
http://dx.doi.org/10.3390/cells9122641
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