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Echovirus-30 Infection Alters Host Proteins in Lipid Rafts at the Cerebrospinal Fluid Barrier In Vitro

Echovirus-30 (E-30) is a non-polio enterovirus responsible for meningitis outbreaks in children worldwide. To gain access to the central nervous system (CNS), E-30 first has to cross the blood-brain barrier (BBB) or the blood-cerebrospinal fluid barrier (BCSFB). E-30 may use lipid rafts of the host...

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Autores principales: Wiatr, Marie, Staubach, Simon, Figueiredo, Ricardo, Stump-Guthier, Carolin, Ishikawa, Hiroshi, Schwerk, Christian, Schroten, Horst, Hanisch, Franz-Georg, Rudolph, Henriette, Tenenbaum, Tobias
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7764136/
https://www.ncbi.nlm.nih.gov/pubmed/33321840
http://dx.doi.org/10.3390/microorganisms8121958
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author Wiatr, Marie
Staubach, Simon
Figueiredo, Ricardo
Stump-Guthier, Carolin
Ishikawa, Hiroshi
Schwerk, Christian
Schroten, Horst
Hanisch, Franz-Georg
Rudolph, Henriette
Tenenbaum, Tobias
author_facet Wiatr, Marie
Staubach, Simon
Figueiredo, Ricardo
Stump-Guthier, Carolin
Ishikawa, Hiroshi
Schwerk, Christian
Schroten, Horst
Hanisch, Franz-Georg
Rudolph, Henriette
Tenenbaum, Tobias
author_sort Wiatr, Marie
collection PubMed
description Echovirus-30 (E-30) is a non-polio enterovirus responsible for meningitis outbreaks in children worldwide. To gain access to the central nervous system (CNS), E-30 first has to cross the blood-brain barrier (BBB) or the blood-cerebrospinal fluid barrier (BCSFB). E-30 may use lipid rafts of the host cells to interact with and to invade the BCSFB. To study enteroviral infection of the BCSFB, an established in vitro model based on human immortalized brain choroid plexus papilloma (HIBCPP) cells has been used. Here, we investigated the impact of E-30 infection on the protein content of the lipid rafts at the BCSFB in vitro. Mass spectrometry analysis following E-30 infection versus uninfected conditions revealed differential abundancy in proteins implicated in cellular adhesion, cytoskeleton remodeling, and endocytosis/vesicle budding. Further, we evaluated the blocking of endocytosis via clathrin/dynamin blocking and its consequences for E-30 induced barrier disruption. Interestingly, blocking of endocytosis had no impact on the capacity of E-30 to induce loss of barrier properties in HIBCPP cells. Altogether, these data highlight the impact of E-30 on HIBCPP cells microdomain as an important factor for host cell alteration.
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spelling pubmed-77641362020-12-27 Echovirus-30 Infection Alters Host Proteins in Lipid Rafts at the Cerebrospinal Fluid Barrier In Vitro Wiatr, Marie Staubach, Simon Figueiredo, Ricardo Stump-Guthier, Carolin Ishikawa, Hiroshi Schwerk, Christian Schroten, Horst Hanisch, Franz-Georg Rudolph, Henriette Tenenbaum, Tobias Microorganisms Article Echovirus-30 (E-30) is a non-polio enterovirus responsible for meningitis outbreaks in children worldwide. To gain access to the central nervous system (CNS), E-30 first has to cross the blood-brain barrier (BBB) or the blood-cerebrospinal fluid barrier (BCSFB). E-30 may use lipid rafts of the host cells to interact with and to invade the BCSFB. To study enteroviral infection of the BCSFB, an established in vitro model based on human immortalized brain choroid plexus papilloma (HIBCPP) cells has been used. Here, we investigated the impact of E-30 infection on the protein content of the lipid rafts at the BCSFB in vitro. Mass spectrometry analysis following E-30 infection versus uninfected conditions revealed differential abundancy in proteins implicated in cellular adhesion, cytoskeleton remodeling, and endocytosis/vesicle budding. Further, we evaluated the blocking of endocytosis via clathrin/dynamin blocking and its consequences for E-30 induced barrier disruption. Interestingly, blocking of endocytosis had no impact on the capacity of E-30 to induce loss of barrier properties in HIBCPP cells. Altogether, these data highlight the impact of E-30 on HIBCPP cells microdomain as an important factor for host cell alteration. MDPI 2020-12-10 /pmc/articles/PMC7764136/ /pubmed/33321840 http://dx.doi.org/10.3390/microorganisms8121958 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wiatr, Marie
Staubach, Simon
Figueiredo, Ricardo
Stump-Guthier, Carolin
Ishikawa, Hiroshi
Schwerk, Christian
Schroten, Horst
Hanisch, Franz-Georg
Rudolph, Henriette
Tenenbaum, Tobias
Echovirus-30 Infection Alters Host Proteins in Lipid Rafts at the Cerebrospinal Fluid Barrier In Vitro
title Echovirus-30 Infection Alters Host Proteins in Lipid Rafts at the Cerebrospinal Fluid Barrier In Vitro
title_full Echovirus-30 Infection Alters Host Proteins in Lipid Rafts at the Cerebrospinal Fluid Barrier In Vitro
title_fullStr Echovirus-30 Infection Alters Host Proteins in Lipid Rafts at the Cerebrospinal Fluid Barrier In Vitro
title_full_unstemmed Echovirus-30 Infection Alters Host Proteins in Lipid Rafts at the Cerebrospinal Fluid Barrier In Vitro
title_short Echovirus-30 Infection Alters Host Proteins in Lipid Rafts at the Cerebrospinal Fluid Barrier In Vitro
title_sort echovirus-30 infection alters host proteins in lipid rafts at the cerebrospinal fluid barrier in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7764136/
https://www.ncbi.nlm.nih.gov/pubmed/33321840
http://dx.doi.org/10.3390/microorganisms8121958
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