Leptin System in Obese Dog Skin: A Pilot Study

SIMPLE SUMMARY: Obesity is a widespread phenomenon in pets and its growing trend is similar to the human one. It can be associated with skin pathologies but there is little information on this field in domestic animals. Since in obesity adipokine plasmatic levels changes, in this study leptin (LEP) system was evaluated in the skin of obese dogs to observe changes in peripheral tissue. LEP is a hormone produced mainly by the adipose tissue and its serum level may reflect body mass index and BCS. LEP is also expressed in the skin and it has a prominent role in the biology of this tissue promoting cell proliferation and regulating the wound healing process. Investigation performed in obese and normal-weight dogs evidenced LEP and leptin receptor (LEPR) immunostaining in several skin structures. As LEP expression regards, differences were non-significant, while the LEPR transcripts appeared 10 fold higher in obesedogs. No differences were observed in the composition of skin associated immune system. The obese group-increased LEPR expression suggests that the receptor modulates the system control. The LEP system changes in the skin under obesity conditions however, the exact role of LEP in obese dog skin needs further insights. ABSTRACT: Obesity predisposes to several health problems including skin diseases. However, information on the relationship between obesity and skin disorders in pets is very scarce. Leptin (LEP) is mainly produced by adipose tissue and has a prominent role in skin biology. This study evaluated the LEP system in the skin of obese dogs compared to normal-weight animals. The investigation was carried out on 10 obese (Obese group) and 10 normal-weight (Normal-weight group) dogs through Real-time PCR and immunohistochemistry. Cells of skin associated immune system were also evaluated. No differences were evidenced between the two groups as well as skin inflammation. LEP differences were no significant, while LEPR transcript appeared 10-fold higher in obesedogs than in normal-weight ones. Immunostaining for both molecules was observed in several skin structures such as the epidermis, hair follicles, and glands. No differences appeared in the skin associated immune system composition. This study is a preliminary report showing that LEP system changes in obese dog skin. The increased LEPR expression observed in the obese group suggests that the receptor plays a modulating role in the system control. However, the exact role of LEPin the skin under obesity conditions needs further elucidation.