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Effect of a Lens Protein in Low-Temperature Culture of Novel Immortalized Human Lens Epithelial Cells (iHLEC-NY2)

The prevalence of nuclear cataracts was observed to be significantly higher among residents of tropical and subtropical regions compared to those of temperate and subarctic regions. We hypothesized that elevated environmental temperatures may pose a risk of nuclear cataract development. The results...

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Autores principales: Yamamoto, Naoki, Takeda, Shun, Hatsusaka, Natsuko, Hiramatsu, Noriko, Nagai, Noriaki, Deguchi, Saori, Nakazawa, Yosuke, Takata, Takumi, Kodera, Sachiko, Hirata, Akimasa, Kubo, Eri, Sasaki, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7764252/
https://www.ncbi.nlm.nih.gov/pubmed/33322631
http://dx.doi.org/10.3390/cells9122670
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author Yamamoto, Naoki
Takeda, Shun
Hatsusaka, Natsuko
Hiramatsu, Noriko
Nagai, Noriaki
Deguchi, Saori
Nakazawa, Yosuke
Takata, Takumi
Kodera, Sachiko
Hirata, Akimasa
Kubo, Eri
Sasaki, Hiroshi
author_facet Yamamoto, Naoki
Takeda, Shun
Hatsusaka, Natsuko
Hiramatsu, Noriko
Nagai, Noriaki
Deguchi, Saori
Nakazawa, Yosuke
Takata, Takumi
Kodera, Sachiko
Hirata, Akimasa
Kubo, Eri
Sasaki, Hiroshi
author_sort Yamamoto, Naoki
collection PubMed
description The prevalence of nuclear cataracts was observed to be significantly higher among residents of tropical and subtropical regions compared to those of temperate and subarctic regions. We hypothesized that elevated environmental temperatures may pose a risk of nuclear cataract development. The results of our in silico simulation revealed that in temperate and tropical regions, the human lens temperature ranges from 35.0 °C to 37.5 °C depending on the environmental temperature. The medium temperature changes during the replacement regularly in the cell culture experiment were carefully monitored using a sensor connected to a thermometer and showed a decrease of 1.9 °C, 3.0 °C, 1.7 °C, and 0.1 °C, after 5 min when setting the temperature of the heat plate device at 35.0 °C, 37.5 °C, 40.0 °C, and 42.5 °C, respectively. In the newly created immortalized human lens epithelial cell line clone NY2 (iHLEC-NY2), the amounts of RNA synthesis of αA crystallin, protein expression, and amyloid β (Aβ)1-40 secreted into the medium were increased at the culture temperature of 37.5 °C compared to 35.0 °C. In short-term culture experiments, the secretion of Aβ1-40 observed in cataracts was increased at 37.5 °C compared to 35.0 °C, suggesting that the long-term exposure to a high-temperature environment may increase the risk of cataracts.
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spelling pubmed-77642522020-12-27 Effect of a Lens Protein in Low-Temperature Culture of Novel Immortalized Human Lens Epithelial Cells (iHLEC-NY2) Yamamoto, Naoki Takeda, Shun Hatsusaka, Natsuko Hiramatsu, Noriko Nagai, Noriaki Deguchi, Saori Nakazawa, Yosuke Takata, Takumi Kodera, Sachiko Hirata, Akimasa Kubo, Eri Sasaki, Hiroshi Cells Article The prevalence of nuclear cataracts was observed to be significantly higher among residents of tropical and subtropical regions compared to those of temperate and subarctic regions. We hypothesized that elevated environmental temperatures may pose a risk of nuclear cataract development. The results of our in silico simulation revealed that in temperate and tropical regions, the human lens temperature ranges from 35.0 °C to 37.5 °C depending on the environmental temperature. The medium temperature changes during the replacement regularly in the cell culture experiment were carefully monitored using a sensor connected to a thermometer and showed a decrease of 1.9 °C, 3.0 °C, 1.7 °C, and 0.1 °C, after 5 min when setting the temperature of the heat plate device at 35.0 °C, 37.5 °C, 40.0 °C, and 42.5 °C, respectively. In the newly created immortalized human lens epithelial cell line clone NY2 (iHLEC-NY2), the amounts of RNA synthesis of αA crystallin, protein expression, and amyloid β (Aβ)1-40 secreted into the medium were increased at the culture temperature of 37.5 °C compared to 35.0 °C. In short-term culture experiments, the secretion of Aβ1-40 observed in cataracts was increased at 37.5 °C compared to 35.0 °C, suggesting that the long-term exposure to a high-temperature environment may increase the risk of cataracts. MDPI 2020-12-11 /pmc/articles/PMC7764252/ /pubmed/33322631 http://dx.doi.org/10.3390/cells9122670 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yamamoto, Naoki
Takeda, Shun
Hatsusaka, Natsuko
Hiramatsu, Noriko
Nagai, Noriaki
Deguchi, Saori
Nakazawa, Yosuke
Takata, Takumi
Kodera, Sachiko
Hirata, Akimasa
Kubo, Eri
Sasaki, Hiroshi
Effect of a Lens Protein in Low-Temperature Culture of Novel Immortalized Human Lens Epithelial Cells (iHLEC-NY2)
title Effect of a Lens Protein in Low-Temperature Culture of Novel Immortalized Human Lens Epithelial Cells (iHLEC-NY2)
title_full Effect of a Lens Protein in Low-Temperature Culture of Novel Immortalized Human Lens Epithelial Cells (iHLEC-NY2)
title_fullStr Effect of a Lens Protein in Low-Temperature Culture of Novel Immortalized Human Lens Epithelial Cells (iHLEC-NY2)
title_full_unstemmed Effect of a Lens Protein in Low-Temperature Culture of Novel Immortalized Human Lens Epithelial Cells (iHLEC-NY2)
title_short Effect of a Lens Protein in Low-Temperature Culture of Novel Immortalized Human Lens Epithelial Cells (iHLEC-NY2)
title_sort effect of a lens protein in low-temperature culture of novel immortalized human lens epithelial cells (ihlec-ny2)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7764252/
https://www.ncbi.nlm.nih.gov/pubmed/33322631
http://dx.doi.org/10.3390/cells9122670
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