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Establishment and Preliminary Characterization of Three Astrocytic Cells Lines Obtained from Primary Rat Astrocytes by Sub-Cloning

Gliomas are complex and heterogeneous tumors that originate from the glial cells of the brain. The malignant cells undergo deep modifications of their metabolism, and acquire the capacity to invade the brain parenchyma and to induce epigenetic modifications in the other brain cell types. In spite of...

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Autores principales: Caradonna, Fabio, Schiera, Gabriella, Di Liegro, Carlo Maria, Vitale, Vincenzo, Cruciata, Ilenia, Ferrara, Tiziana, D’Oca, Pietro, Mormino, Riccardo, Rizzo, Simona Maria Angela, Di Liegro, Italia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7764261/
https://www.ncbi.nlm.nih.gov/pubmed/33322092
http://dx.doi.org/10.3390/genes11121502
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author Caradonna, Fabio
Schiera, Gabriella
Di Liegro, Carlo Maria
Vitale, Vincenzo
Cruciata, Ilenia
Ferrara, Tiziana
D’Oca, Pietro
Mormino, Riccardo
Rizzo, Simona Maria Angela
Di Liegro, Italia
author_facet Caradonna, Fabio
Schiera, Gabriella
Di Liegro, Carlo Maria
Vitale, Vincenzo
Cruciata, Ilenia
Ferrara, Tiziana
D’Oca, Pietro
Mormino, Riccardo
Rizzo, Simona Maria Angela
Di Liegro, Italia
author_sort Caradonna, Fabio
collection PubMed
description Gliomas are complex and heterogeneous tumors that originate from the glial cells of the brain. The malignant cells undergo deep modifications of their metabolism, and acquire the capacity to invade the brain parenchyma and to induce epigenetic modifications in the other brain cell types. In spite of the efforts made to define the pathology at the molecular level, and to set novel approaches to reach the infiltrating cells, gliomas are still fatal. In order to gain a better knowledge of the cellular events that accompany astrocyte transformation, we developed three increasingly transformed astrocyte cell lines, starting from primary rat cortical astrocytes, and analyzed them at the cytogenetic and epigenetic level. In parallel, we also studied the expression of the differentiation-related H1.0 linker histone variant to evaluate its possible modification in relation with transformation. We found that the most modified astrocytes (A-FC6) have epigenetic and chromosomal alterations typical of cancer, and that the other two clones (A-GS1 and A-VV5) have intermediate properties. Surprisingly, the differentiation-specific somatic histone H1.0 steadily increases from the normal astrocytes to the most transformed ones. As a whole, our results suggest that these three cell lines, together with the starting primary cells, constitute a potential model for studying glioma development.
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spelling pubmed-77642612020-12-27 Establishment and Preliminary Characterization of Three Astrocytic Cells Lines Obtained from Primary Rat Astrocytes by Sub-Cloning Caradonna, Fabio Schiera, Gabriella Di Liegro, Carlo Maria Vitale, Vincenzo Cruciata, Ilenia Ferrara, Tiziana D’Oca, Pietro Mormino, Riccardo Rizzo, Simona Maria Angela Di Liegro, Italia Genes (Basel) Article Gliomas are complex and heterogeneous tumors that originate from the glial cells of the brain. The malignant cells undergo deep modifications of their metabolism, and acquire the capacity to invade the brain parenchyma and to induce epigenetic modifications in the other brain cell types. In spite of the efforts made to define the pathology at the molecular level, and to set novel approaches to reach the infiltrating cells, gliomas are still fatal. In order to gain a better knowledge of the cellular events that accompany astrocyte transformation, we developed three increasingly transformed astrocyte cell lines, starting from primary rat cortical astrocytes, and analyzed them at the cytogenetic and epigenetic level. In parallel, we also studied the expression of the differentiation-related H1.0 linker histone variant to evaluate its possible modification in relation with transformation. We found that the most modified astrocytes (A-FC6) have epigenetic and chromosomal alterations typical of cancer, and that the other two clones (A-GS1 and A-VV5) have intermediate properties. Surprisingly, the differentiation-specific somatic histone H1.0 steadily increases from the normal astrocytes to the most transformed ones. As a whole, our results suggest that these three cell lines, together with the starting primary cells, constitute a potential model for studying glioma development. MDPI 2020-12-13 /pmc/articles/PMC7764261/ /pubmed/33322092 http://dx.doi.org/10.3390/genes11121502 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Caradonna, Fabio
Schiera, Gabriella
Di Liegro, Carlo Maria
Vitale, Vincenzo
Cruciata, Ilenia
Ferrara, Tiziana
D’Oca, Pietro
Mormino, Riccardo
Rizzo, Simona Maria Angela
Di Liegro, Italia
Establishment and Preliminary Characterization of Three Astrocytic Cells Lines Obtained from Primary Rat Astrocytes by Sub-Cloning
title Establishment and Preliminary Characterization of Three Astrocytic Cells Lines Obtained from Primary Rat Astrocytes by Sub-Cloning
title_full Establishment and Preliminary Characterization of Three Astrocytic Cells Lines Obtained from Primary Rat Astrocytes by Sub-Cloning
title_fullStr Establishment and Preliminary Characterization of Three Astrocytic Cells Lines Obtained from Primary Rat Astrocytes by Sub-Cloning
title_full_unstemmed Establishment and Preliminary Characterization of Three Astrocytic Cells Lines Obtained from Primary Rat Astrocytes by Sub-Cloning
title_short Establishment and Preliminary Characterization of Three Astrocytic Cells Lines Obtained from Primary Rat Astrocytes by Sub-Cloning
title_sort establishment and preliminary characterization of three astrocytic cells lines obtained from primary rat astrocytes by sub-cloning
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7764261/
https://www.ncbi.nlm.nih.gov/pubmed/33322092
http://dx.doi.org/10.3390/genes11121502
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