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Determination of the Synthetic Cannabinoids JWH-122, JWH-210, UR-144 in Oral Fluid of Consumers by GC-MS and Quantification of Parent Compounds and Metabolites by UHPLC-MS/MS

The consumption of synthetic cannabinoids (SCs) has significantly increased in the last decade and the analysis of SCs and their metabolites in human specimens is gaining interest in clinical and forensic toxicology. A pilot study has been carried out using a combination of an initial last generatio...

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Autores principales: La Maida, Nunzia, Pellegrini, Manuela, Papaseit, Esther, Pérez-Mañá, Clara, Poyatos, Lourdes, Ventura, Mireia, Galindo, Liliana, Busardò, Francesco Paolo, Pichini, Simona, Farré, Magí, Marchei, Emilia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7764284/
https://www.ncbi.nlm.nih.gov/pubmed/33321929
http://dx.doi.org/10.3390/ijms21249414
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author La Maida, Nunzia
Pellegrini, Manuela
Papaseit, Esther
Pérez-Mañá, Clara
Poyatos, Lourdes
Ventura, Mireia
Galindo, Liliana
Busardò, Francesco Paolo
Pichini, Simona
Farré, Magí
Marchei, Emilia
author_facet La Maida, Nunzia
Pellegrini, Manuela
Papaseit, Esther
Pérez-Mañá, Clara
Poyatos, Lourdes
Ventura, Mireia
Galindo, Liliana
Busardò, Francesco Paolo
Pichini, Simona
Farré, Magí
Marchei, Emilia
author_sort La Maida, Nunzia
collection PubMed
description The consumption of synthetic cannabinoids (SCs) has significantly increased in the last decade and the analysis of SCs and their metabolites in human specimens is gaining interest in clinical and forensic toxicology. A pilot study has been carried out using a combination of an initial last generation gas chromatography-mass spectrometry (GC-MS) screening method for the determination of JWH-122, JWH-210, UR-144) in oral fluid (OF) of consumers and an ultra-high performance liquid chromatography high resolution mass spectrometry (UHPLC-HRMS) confirmatory method for the quantification of the parent compounds and their metabolites in the same biological matrix. OF samples were simply liquid-liquid extracted before injecting in both chromatographic systems. The developed methods have been successfully validated and were linear from limit of quantification (LOQ) to 50 ng/mL OF. Recovery of analytes was always higher than 70% and matrix effect always lower than 15% whereas intra-assay and inter-assay precision and accuracy were always better than 16%. After smoking 1 mg JWH-122 or UR-144 and 3 mg JWH-210, maximum concentration of 4.00–3.14 ng/mL JWH-122, 8.10–7.30 ng/mL JWH-210 ng/mL and 7.40 and 6.81 ng/mL UR-144 were measured by GC-MS and UHPLC-HRMS respectively at 20 min after inhalation. Metabolites of JWH 122 and 210 were quantified in OF by UHPLC-HRMS, while that of UR144 was only detectable in traces. Our results provide for the first time information about disposition of these SCs and their metabolites in consumers OF. Last generation GC-MS has proven useful tool to identify and quantify parent SCs whereas UHPLC-HRMS also confirmed the presence of SCs metabolites in the OF of SCs consumers.
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spelling pubmed-77642842020-12-27 Determination of the Synthetic Cannabinoids JWH-122, JWH-210, UR-144 in Oral Fluid of Consumers by GC-MS and Quantification of Parent Compounds and Metabolites by UHPLC-MS/MS La Maida, Nunzia Pellegrini, Manuela Papaseit, Esther Pérez-Mañá, Clara Poyatos, Lourdes Ventura, Mireia Galindo, Liliana Busardò, Francesco Paolo Pichini, Simona Farré, Magí Marchei, Emilia Int J Mol Sci Article The consumption of synthetic cannabinoids (SCs) has significantly increased in the last decade and the analysis of SCs and their metabolites in human specimens is gaining interest in clinical and forensic toxicology. A pilot study has been carried out using a combination of an initial last generation gas chromatography-mass spectrometry (GC-MS) screening method for the determination of JWH-122, JWH-210, UR-144) in oral fluid (OF) of consumers and an ultra-high performance liquid chromatography high resolution mass spectrometry (UHPLC-HRMS) confirmatory method for the quantification of the parent compounds and their metabolites in the same biological matrix. OF samples were simply liquid-liquid extracted before injecting in both chromatographic systems. The developed methods have been successfully validated and were linear from limit of quantification (LOQ) to 50 ng/mL OF. Recovery of analytes was always higher than 70% and matrix effect always lower than 15% whereas intra-assay and inter-assay precision and accuracy were always better than 16%. After smoking 1 mg JWH-122 or UR-144 and 3 mg JWH-210, maximum concentration of 4.00–3.14 ng/mL JWH-122, 8.10–7.30 ng/mL JWH-210 ng/mL and 7.40 and 6.81 ng/mL UR-144 were measured by GC-MS and UHPLC-HRMS respectively at 20 min after inhalation. Metabolites of JWH 122 and 210 were quantified in OF by UHPLC-HRMS, while that of UR144 was only detectable in traces. Our results provide for the first time information about disposition of these SCs and their metabolites in consumers OF. Last generation GC-MS has proven useful tool to identify and quantify parent SCs whereas UHPLC-HRMS also confirmed the presence of SCs metabolites in the OF of SCs consumers. MDPI 2020-12-10 /pmc/articles/PMC7764284/ /pubmed/33321929 http://dx.doi.org/10.3390/ijms21249414 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
La Maida, Nunzia
Pellegrini, Manuela
Papaseit, Esther
Pérez-Mañá, Clara
Poyatos, Lourdes
Ventura, Mireia
Galindo, Liliana
Busardò, Francesco Paolo
Pichini, Simona
Farré, Magí
Marchei, Emilia
Determination of the Synthetic Cannabinoids JWH-122, JWH-210, UR-144 in Oral Fluid of Consumers by GC-MS and Quantification of Parent Compounds and Metabolites by UHPLC-MS/MS
title Determination of the Synthetic Cannabinoids JWH-122, JWH-210, UR-144 in Oral Fluid of Consumers by GC-MS and Quantification of Parent Compounds and Metabolites by UHPLC-MS/MS
title_full Determination of the Synthetic Cannabinoids JWH-122, JWH-210, UR-144 in Oral Fluid of Consumers by GC-MS and Quantification of Parent Compounds and Metabolites by UHPLC-MS/MS
title_fullStr Determination of the Synthetic Cannabinoids JWH-122, JWH-210, UR-144 in Oral Fluid of Consumers by GC-MS and Quantification of Parent Compounds and Metabolites by UHPLC-MS/MS
title_full_unstemmed Determination of the Synthetic Cannabinoids JWH-122, JWH-210, UR-144 in Oral Fluid of Consumers by GC-MS and Quantification of Parent Compounds and Metabolites by UHPLC-MS/MS
title_short Determination of the Synthetic Cannabinoids JWH-122, JWH-210, UR-144 in Oral Fluid of Consumers by GC-MS and Quantification of Parent Compounds and Metabolites by UHPLC-MS/MS
title_sort determination of the synthetic cannabinoids jwh-122, jwh-210, ur-144 in oral fluid of consumers by gc-ms and quantification of parent compounds and metabolites by uhplc-ms/ms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7764284/
https://www.ncbi.nlm.nih.gov/pubmed/33321929
http://dx.doi.org/10.3390/ijms21249414
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