Cargando…
Eggshell Membrane/Gellan Gum Composite Hydrogels with Increased Degradability, Biocompatibility, and Anti-Swelling Properties for Effective Regeneration of Retinal Pigment Epithelium
A gellan gum (GG) hydrogel must demonstrate a number of critical qualities—low viscosity, degradability, desirable mechanical properties, anti-swelling properties, and biocompatibility—in order to be regarded as suitable for retinal pigment epithelium (RPE) regeneration. In this study, we investigat...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7764595/ https://www.ncbi.nlm.nih.gov/pubmed/33317040 http://dx.doi.org/10.3390/polym12122941 |
_version_ | 1783628293911609344 |
---|---|
author | Choi, Jeongmin Lee, Jaewoo Shin, Myeong Eun Been, Suyoung Lee, Dae Hoon Khang, Gilson |
author_facet | Choi, Jeongmin Lee, Jaewoo Shin, Myeong Eun Been, Suyoung Lee, Dae Hoon Khang, Gilson |
author_sort | Choi, Jeongmin |
collection | PubMed |
description | A gellan gum (GG) hydrogel must demonstrate a number of critical qualities—low viscosity, degradability, desirable mechanical properties, anti-swelling properties, and biocompatibility—in order to be regarded as suitable for retinal pigment epithelium (RPE) regeneration. In this study, we investigated whether the application of an eggshell membrane (ESM) to a GG hydrogel improved these critical attributes. The crosslinking of the ESM/GG hydrogels was most effectively reduced, when a 4 w/v% ESM was used, leading to a 40% less viscosity and a 30% higher degradation efficiency than a pure GG hydrogel. The compressive moduli of the ESM/GG hydrogels were maintained, as the smaller pores formed by the addition of the ESM compensated for the slightly weakened mechanical properties of the ESM/GG hydrogels. Meanwhile, due to the relatively low hydrophilicity of ESM, a 4 w/v% ESM enabled an ESM/GG hydrogel to swell 30% less than a pure GG hydrogel. Finally, the similarity in components between the ESM and RPE cells facilitated the proliferation of the latter without any significant cytotoxicity. |
format | Online Article Text |
id | pubmed-7764595 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77645952020-12-27 Eggshell Membrane/Gellan Gum Composite Hydrogels with Increased Degradability, Biocompatibility, and Anti-Swelling Properties for Effective Regeneration of Retinal Pigment Epithelium Choi, Jeongmin Lee, Jaewoo Shin, Myeong Eun Been, Suyoung Lee, Dae Hoon Khang, Gilson Polymers (Basel) Article A gellan gum (GG) hydrogel must demonstrate a number of critical qualities—low viscosity, degradability, desirable mechanical properties, anti-swelling properties, and biocompatibility—in order to be regarded as suitable for retinal pigment epithelium (RPE) regeneration. In this study, we investigated whether the application of an eggshell membrane (ESM) to a GG hydrogel improved these critical attributes. The crosslinking of the ESM/GG hydrogels was most effectively reduced, when a 4 w/v% ESM was used, leading to a 40% less viscosity and a 30% higher degradation efficiency than a pure GG hydrogel. The compressive moduli of the ESM/GG hydrogels were maintained, as the smaller pores formed by the addition of the ESM compensated for the slightly weakened mechanical properties of the ESM/GG hydrogels. Meanwhile, due to the relatively low hydrophilicity of ESM, a 4 w/v% ESM enabled an ESM/GG hydrogel to swell 30% less than a pure GG hydrogel. Finally, the similarity in components between the ESM and RPE cells facilitated the proliferation of the latter without any significant cytotoxicity. MDPI 2020-12-09 /pmc/articles/PMC7764595/ /pubmed/33317040 http://dx.doi.org/10.3390/polym12122941 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Choi, Jeongmin Lee, Jaewoo Shin, Myeong Eun Been, Suyoung Lee, Dae Hoon Khang, Gilson Eggshell Membrane/Gellan Gum Composite Hydrogels with Increased Degradability, Biocompatibility, and Anti-Swelling Properties for Effective Regeneration of Retinal Pigment Epithelium |
title | Eggshell Membrane/Gellan Gum Composite Hydrogels with Increased Degradability, Biocompatibility, and Anti-Swelling Properties for Effective Regeneration of Retinal Pigment Epithelium |
title_full | Eggshell Membrane/Gellan Gum Composite Hydrogels with Increased Degradability, Biocompatibility, and Anti-Swelling Properties for Effective Regeneration of Retinal Pigment Epithelium |
title_fullStr | Eggshell Membrane/Gellan Gum Composite Hydrogels with Increased Degradability, Biocompatibility, and Anti-Swelling Properties for Effective Regeneration of Retinal Pigment Epithelium |
title_full_unstemmed | Eggshell Membrane/Gellan Gum Composite Hydrogels with Increased Degradability, Biocompatibility, and Anti-Swelling Properties for Effective Regeneration of Retinal Pigment Epithelium |
title_short | Eggshell Membrane/Gellan Gum Composite Hydrogels with Increased Degradability, Biocompatibility, and Anti-Swelling Properties for Effective Regeneration of Retinal Pigment Epithelium |
title_sort | eggshell membrane/gellan gum composite hydrogels with increased degradability, biocompatibility, and anti-swelling properties for effective regeneration of retinal pigment epithelium |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7764595/ https://www.ncbi.nlm.nih.gov/pubmed/33317040 http://dx.doi.org/10.3390/polym12122941 |
work_keys_str_mv | AT choijeongmin eggshellmembranegellangumcompositehydrogelswithincreaseddegradabilitybiocompatibilityandantiswellingpropertiesforeffectiveregenerationofretinalpigmentepithelium AT leejaewoo eggshellmembranegellangumcompositehydrogelswithincreaseddegradabilitybiocompatibilityandantiswellingpropertiesforeffectiveregenerationofretinalpigmentepithelium AT shinmyeongeun eggshellmembranegellangumcompositehydrogelswithincreaseddegradabilitybiocompatibilityandantiswellingpropertiesforeffectiveregenerationofretinalpigmentepithelium AT beensuyoung eggshellmembranegellangumcompositehydrogelswithincreaseddegradabilitybiocompatibilityandantiswellingpropertiesforeffectiveregenerationofretinalpigmentepithelium AT leedaehoon eggshellmembranegellangumcompositehydrogelswithincreaseddegradabilitybiocompatibilityandantiswellingpropertiesforeffectiveregenerationofretinalpigmentepithelium AT khanggilson eggshellmembranegellangumcompositehydrogelswithincreaseddegradabilitybiocompatibilityandantiswellingpropertiesforeffectiveregenerationofretinalpigmentepithelium |