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Expression of Circulating MicroRNAs Linked to Bone Metabolism in Chronic Kidney Disease-Mineral and Bone Disorder

The pathophysiology of chronic kidney disease–mineral and bone disorder (CKD-MBD) is complex and multifactorial. Recent studies have identified a link between microRNAs (miRNAs) and bone loss. In this study, we investigated the expression of miRNAs in CKD-MBD. In this case-control study, we included...

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Autores principales: Yavropoulou, Maria P., Vaios, Vasilios, Makras, Polyzois, Georgianos, Panagiotis, Batas, Anastasios, Tsalikakis, Dimitrios, Tzallas, Alexandros, Ntritsos, Georgios, Roumeliotis, Stefanos, Eleftheriadis, Theodoros, Liakopoulos, Vassilios
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7764659/
https://www.ncbi.nlm.nih.gov/pubmed/33322822
http://dx.doi.org/10.3390/biomedicines8120601
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author Yavropoulou, Maria P.
Vaios, Vasilios
Makras, Polyzois
Georgianos, Panagiotis
Batas, Anastasios
Tsalikakis, Dimitrios
Tzallas, Alexandros
Ntritsos, Georgios
Roumeliotis, Stefanos
Eleftheriadis, Theodoros
Liakopoulos, Vassilios
author_facet Yavropoulou, Maria P.
Vaios, Vasilios
Makras, Polyzois
Georgianos, Panagiotis
Batas, Anastasios
Tsalikakis, Dimitrios
Tzallas, Alexandros
Ntritsos, Georgios
Roumeliotis, Stefanos
Eleftheriadis, Theodoros
Liakopoulos, Vassilios
author_sort Yavropoulou, Maria P.
collection PubMed
description The pathophysiology of chronic kidney disease–mineral and bone disorder (CKD-MBD) is complex and multifactorial. Recent studies have identified a link between microRNAs (miRNAs) and bone loss. In this study, we investigated the expression of miRNAs in CKD-MBD. In this case-control study, we included thirty patients with CKD-MBD (cases) and 30 age- and gender-matched healthy individuals (controls). Bone mineral density (BMD) and trabecular bone score (TBS) evaluation was performed with dual X-ray absorptiometry. The selected panel of miRNAs included: hsa-miRNA-21-5p; hsa-miRNA-23a-3p; hsa-miRNA-24-2-5p; hsa-miRNA-26a-5p; hsa-miRNA-29a-3; hsa-miRNA-124-3p; hsa-miRNA-2861. The majority of cases had low BMD values. The relative expression of miRNA-21-5p was 15 times lower [fold regulation (FR): −14.7 ± 8.1, p = 0.034), miRNA-124-3p, 6 times lower (FR: −5.9 ± 4, p = 0.005), and miRNA-23a-3p, 4 times lower (FR: −3.8 ± 2.0, p = 0.036) in cases compared to controls. MiRNA-23a-3p was significantly and inversely correlated with TBS, adjusted for calcium metabolism and BMD values (beta = −0.221, p = 0.003, 95% CI −0.360, −0,081) in cases. In a receiver operating characteristic (ROC) analysis, expression of miRNA-124-3p demonstrated 78% sensitivity and 83% specificity in identifying CKD patents with osteoporosis. Serum expression of miRNAs related to osteoblasts (miRNA-23a-3p) and osteoclasts (miRNA-21-5p, miRNA-124-3p) is significantly altered in patients with CKD-MBD.
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spelling pubmed-77646592020-12-27 Expression of Circulating MicroRNAs Linked to Bone Metabolism in Chronic Kidney Disease-Mineral and Bone Disorder Yavropoulou, Maria P. Vaios, Vasilios Makras, Polyzois Georgianos, Panagiotis Batas, Anastasios Tsalikakis, Dimitrios Tzallas, Alexandros Ntritsos, Georgios Roumeliotis, Stefanos Eleftheriadis, Theodoros Liakopoulos, Vassilios Biomedicines Article The pathophysiology of chronic kidney disease–mineral and bone disorder (CKD-MBD) is complex and multifactorial. Recent studies have identified a link between microRNAs (miRNAs) and bone loss. In this study, we investigated the expression of miRNAs in CKD-MBD. In this case-control study, we included thirty patients with CKD-MBD (cases) and 30 age- and gender-matched healthy individuals (controls). Bone mineral density (BMD) and trabecular bone score (TBS) evaluation was performed with dual X-ray absorptiometry. The selected panel of miRNAs included: hsa-miRNA-21-5p; hsa-miRNA-23a-3p; hsa-miRNA-24-2-5p; hsa-miRNA-26a-5p; hsa-miRNA-29a-3; hsa-miRNA-124-3p; hsa-miRNA-2861. The majority of cases had low BMD values. The relative expression of miRNA-21-5p was 15 times lower [fold regulation (FR): −14.7 ± 8.1, p = 0.034), miRNA-124-3p, 6 times lower (FR: −5.9 ± 4, p = 0.005), and miRNA-23a-3p, 4 times lower (FR: −3.8 ± 2.0, p = 0.036) in cases compared to controls. MiRNA-23a-3p was significantly and inversely correlated with TBS, adjusted for calcium metabolism and BMD values (beta = −0.221, p = 0.003, 95% CI −0.360, −0,081) in cases. In a receiver operating characteristic (ROC) analysis, expression of miRNA-124-3p demonstrated 78% sensitivity and 83% specificity in identifying CKD patents with osteoporosis. Serum expression of miRNAs related to osteoblasts (miRNA-23a-3p) and osteoclasts (miRNA-21-5p, miRNA-124-3p) is significantly altered in patients with CKD-MBD. MDPI 2020-12-12 /pmc/articles/PMC7764659/ /pubmed/33322822 http://dx.doi.org/10.3390/biomedicines8120601 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yavropoulou, Maria P.
Vaios, Vasilios
Makras, Polyzois
Georgianos, Panagiotis
Batas, Anastasios
Tsalikakis, Dimitrios
Tzallas, Alexandros
Ntritsos, Georgios
Roumeliotis, Stefanos
Eleftheriadis, Theodoros
Liakopoulos, Vassilios
Expression of Circulating MicroRNAs Linked to Bone Metabolism in Chronic Kidney Disease-Mineral and Bone Disorder
title Expression of Circulating MicroRNAs Linked to Bone Metabolism in Chronic Kidney Disease-Mineral and Bone Disorder
title_full Expression of Circulating MicroRNAs Linked to Bone Metabolism in Chronic Kidney Disease-Mineral and Bone Disorder
title_fullStr Expression of Circulating MicroRNAs Linked to Bone Metabolism in Chronic Kidney Disease-Mineral and Bone Disorder
title_full_unstemmed Expression of Circulating MicroRNAs Linked to Bone Metabolism in Chronic Kidney Disease-Mineral and Bone Disorder
title_short Expression of Circulating MicroRNAs Linked to Bone Metabolism in Chronic Kidney Disease-Mineral and Bone Disorder
title_sort expression of circulating micrornas linked to bone metabolism in chronic kidney disease-mineral and bone disorder
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7764659/
https://www.ncbi.nlm.nih.gov/pubmed/33322822
http://dx.doi.org/10.3390/biomedicines8120601
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