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IgA2 Antibodies against SARS-CoV-2 Correlate with NET Formation and Fatal Outcome in Severely Diseased COVID-19 Patients
Infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) leads to an adaptive immune response in the host and the formation of anti-SARS-CoV-2 specific antibodies. While IgG responses against SARS-CoV-2 have been characterized quite well, less is known about IgA. IgA2 activates im...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7764693/ https://www.ncbi.nlm.nih.gov/pubmed/33322797 http://dx.doi.org/10.3390/cells9122676 |
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author | Staats, Léonie A. N. Pfeiffer, Hella Knopf, Jasmin Lindemann, Aylin Fürst, Julia Kremer, Andreas E. Hackstein, Holger Neurath, Markus F. Muñoz, Luis E. Achenbach, Susanne Leppkes, Moritz Herrmann, Martin Schett, Georg Steffen, Ulrike |
author_facet | Staats, Léonie A. N. Pfeiffer, Hella Knopf, Jasmin Lindemann, Aylin Fürst, Julia Kremer, Andreas E. Hackstein, Holger Neurath, Markus F. Muñoz, Luis E. Achenbach, Susanne Leppkes, Moritz Herrmann, Martin Schett, Georg Steffen, Ulrike |
author_sort | Staats, Léonie A. N. |
collection | PubMed |
description | Infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) leads to an adaptive immune response in the host and the formation of anti-SARS-CoV-2 specific antibodies. While IgG responses against SARS-CoV-2 have been characterized quite well, less is known about IgA. IgA2 activates immune cells and induces inflammation and neutrophil extracellular trap (NET) formation which may contribute to organ injury and fatal outcome in SARS-CoV-2-infected patients. SARS-CoV-2 spike protein specific antibody levels were measured in plasma samples of 15 noninfected controls and 82 SARS-CoV-2-infected patients with no or mild symptoms, moderate symptoms (hospitalization) or severe disease (intensive care unit, ICU). Antibody levels were compared to levels of C-reactive protein (CRP) and circulating extracellular DNA (ecDNA) as markers for general inflammation and NET formation, respectively. While levels of SARS-CoV-2-specific IgG were similar in all patient groups, IgA2 antibodies were restricted to severe disease and showed the strongest discrimination between nonfatal and fatal outcome in patients with severe SARS-CoV-2 infection. While anti-SARS-CoV-2 IgG and IgA2 levels correlated with CRP levels in severely diseased patients, only anti-SARS-CoV-2 IgA2 correlated with ecDNA. These data suggest that the formation of anti-SARS-CoV-2 IgA2 during SARS-CoV-2 infection is a marker for more severe disease related to NET formation and poor outcome. |
format | Online Article Text |
id | pubmed-7764693 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77646932020-12-27 IgA2 Antibodies against SARS-CoV-2 Correlate with NET Formation and Fatal Outcome in Severely Diseased COVID-19 Patients Staats, Léonie A. N. Pfeiffer, Hella Knopf, Jasmin Lindemann, Aylin Fürst, Julia Kremer, Andreas E. Hackstein, Holger Neurath, Markus F. Muñoz, Luis E. Achenbach, Susanne Leppkes, Moritz Herrmann, Martin Schett, Georg Steffen, Ulrike Cells Communication Infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) leads to an adaptive immune response in the host and the formation of anti-SARS-CoV-2 specific antibodies. While IgG responses against SARS-CoV-2 have been characterized quite well, less is known about IgA. IgA2 activates immune cells and induces inflammation and neutrophil extracellular trap (NET) formation which may contribute to organ injury and fatal outcome in SARS-CoV-2-infected patients. SARS-CoV-2 spike protein specific antibody levels were measured in plasma samples of 15 noninfected controls and 82 SARS-CoV-2-infected patients with no or mild symptoms, moderate symptoms (hospitalization) or severe disease (intensive care unit, ICU). Antibody levels were compared to levels of C-reactive protein (CRP) and circulating extracellular DNA (ecDNA) as markers for general inflammation and NET formation, respectively. While levels of SARS-CoV-2-specific IgG were similar in all patient groups, IgA2 antibodies were restricted to severe disease and showed the strongest discrimination between nonfatal and fatal outcome in patients with severe SARS-CoV-2 infection. While anti-SARS-CoV-2 IgG and IgA2 levels correlated with CRP levels in severely diseased patients, only anti-SARS-CoV-2 IgA2 correlated with ecDNA. These data suggest that the formation of anti-SARS-CoV-2 IgA2 during SARS-CoV-2 infection is a marker for more severe disease related to NET formation and poor outcome. MDPI 2020-12-12 /pmc/articles/PMC7764693/ /pubmed/33322797 http://dx.doi.org/10.3390/cells9122676 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Staats, Léonie A. N. Pfeiffer, Hella Knopf, Jasmin Lindemann, Aylin Fürst, Julia Kremer, Andreas E. Hackstein, Holger Neurath, Markus F. Muñoz, Luis E. Achenbach, Susanne Leppkes, Moritz Herrmann, Martin Schett, Georg Steffen, Ulrike IgA2 Antibodies against SARS-CoV-2 Correlate with NET Formation and Fatal Outcome in Severely Diseased COVID-19 Patients |
title | IgA2 Antibodies against SARS-CoV-2 Correlate with NET Formation and Fatal Outcome in Severely Diseased COVID-19 Patients |
title_full | IgA2 Antibodies against SARS-CoV-2 Correlate with NET Formation and Fatal Outcome in Severely Diseased COVID-19 Patients |
title_fullStr | IgA2 Antibodies against SARS-CoV-2 Correlate with NET Formation and Fatal Outcome in Severely Diseased COVID-19 Patients |
title_full_unstemmed | IgA2 Antibodies against SARS-CoV-2 Correlate with NET Formation and Fatal Outcome in Severely Diseased COVID-19 Patients |
title_short | IgA2 Antibodies against SARS-CoV-2 Correlate with NET Formation and Fatal Outcome in Severely Diseased COVID-19 Patients |
title_sort | iga2 antibodies against sars-cov-2 correlate with net formation and fatal outcome in severely diseased covid-19 patients |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7764693/ https://www.ncbi.nlm.nih.gov/pubmed/33322797 http://dx.doi.org/10.3390/cells9122676 |
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