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Preliminary Study on the Identification of BRAF(V600E) Mutation in Colorectal Cancer by Near-Infrared Spectroscopy

INTRODUCTION: In metastatic colorectal cancer (mCRC), the B-type Raf kinase (BRAF)(V600E) mutation is a molecular biomarker of poor prognosis and is of great importance to drug target. Currently, the commonly used methods for detecting BRAF(V600E) mutation include immunohistochemistry (IHC) and gene...

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Autores principales: Duan, Jiale, Yang, Yanping, Yin, Lei, Zhang, Xue, Tang, Yi, Zhang, Shuxian, Gong, Hanjuan, Xiao, Ming, Li, Ming, Li, Qingshu, Li, Xian, Yang, Lian, Fan, Qi, Wang, Yalan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7764696/
https://www.ncbi.nlm.nih.gov/pubmed/33376356
http://dx.doi.org/10.2147/OTT.S287814
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author Duan, Jiale
Yang, Yanping
Yin, Lei
Zhang, Xue
Tang, Yi
Zhang, Shuxian
Gong, Hanjuan
Xiao, Ming
Li, Ming
Li, Qingshu
Li, Xian
Yang, Lian
Fan, Qi
Wang, Yalan
author_facet Duan, Jiale
Yang, Yanping
Yin, Lei
Zhang, Xue
Tang, Yi
Zhang, Shuxian
Gong, Hanjuan
Xiao, Ming
Li, Ming
Li, Qingshu
Li, Xian
Yang, Lian
Fan, Qi
Wang, Yalan
author_sort Duan, Jiale
collection PubMed
description INTRODUCTION: In metastatic colorectal cancer (mCRC), the B-type Raf kinase (BRAF)(V600E) mutation is a molecular biomarker of poor prognosis and is of great importance to drug target. Currently, the commonly used methods for detecting BRAF(V600E) mutation include immunohistochemistry (IHC) and gene sequencing, but both present certain limitations. Near-infrared (NIR) spectroscopy is a spectroscopy technology that takes advantage of the electromagnetic wavelength between visible light and mid-infrared light. METHODS: IHC was used to detect the expression of BRAF(V600E) protein with the BRAF(V600E) (VE1) antibody in 42 cases of paraffin-embedded (FFPE) mCRC tissue sections. The NIR-discriminant analysis model (NIRS-DA) was established using 6 cases of wild-type and 6 cases of mutant-type BRAF specimens. RESULTS: IHC detection results revealed 13 cases of weakly positive (+), 1 case of moderately positive (++), and 28 cases of negative (−) CRC. Compared with the next-generation sequencing (NGS) results, the positive rate was 66.7%. The classification accuracy of calibration (CAC) was 100% compared with the results of NGS, demonstrating that the BRAF(V600E) mutant NIRS-DA model, verified by 2 cases of wild-type and 2 cases of mutant-type CRC samples was established. The NIRS-DA model was used to predict gene mutation in the CRC samples, 7 cases were positive (+), and 35 cases were negative (−), and the classification accuracy of prediction (CAP) was 83.3% (35/42). DISCUSSION: The NIRS-DA model-predicted results were in high agreement with the detection results of NGS, and the difference in IHC is not statistically significant (P>0.05). However, this study is a preliminary discussion on a methodology due to its small sample size.
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spelling pubmed-77646962020-12-28 Preliminary Study on the Identification of BRAF(V600E) Mutation in Colorectal Cancer by Near-Infrared Spectroscopy Duan, Jiale Yang, Yanping Yin, Lei Zhang, Xue Tang, Yi Zhang, Shuxian Gong, Hanjuan Xiao, Ming Li, Ming Li, Qingshu Li, Xian Yang, Lian Fan, Qi Wang, Yalan Onco Targets Ther Original Research INTRODUCTION: In metastatic colorectal cancer (mCRC), the B-type Raf kinase (BRAF)(V600E) mutation is a molecular biomarker of poor prognosis and is of great importance to drug target. Currently, the commonly used methods for detecting BRAF(V600E) mutation include immunohistochemistry (IHC) and gene sequencing, but both present certain limitations. Near-infrared (NIR) spectroscopy is a spectroscopy technology that takes advantage of the electromagnetic wavelength between visible light and mid-infrared light. METHODS: IHC was used to detect the expression of BRAF(V600E) protein with the BRAF(V600E) (VE1) antibody in 42 cases of paraffin-embedded (FFPE) mCRC tissue sections. The NIR-discriminant analysis model (NIRS-DA) was established using 6 cases of wild-type and 6 cases of mutant-type BRAF specimens. RESULTS: IHC detection results revealed 13 cases of weakly positive (+), 1 case of moderately positive (++), and 28 cases of negative (−) CRC. Compared with the next-generation sequencing (NGS) results, the positive rate was 66.7%. The classification accuracy of calibration (CAC) was 100% compared with the results of NGS, demonstrating that the BRAF(V600E) mutant NIRS-DA model, verified by 2 cases of wild-type and 2 cases of mutant-type CRC samples was established. The NIRS-DA model was used to predict gene mutation in the CRC samples, 7 cases were positive (+), and 35 cases were negative (−), and the classification accuracy of prediction (CAP) was 83.3% (35/42). DISCUSSION: The NIRS-DA model-predicted results were in high agreement with the detection results of NGS, and the difference in IHC is not statistically significant (P>0.05). However, this study is a preliminary discussion on a methodology due to its small sample size. Dove 2020-12-22 /pmc/articles/PMC7764696/ /pubmed/33376356 http://dx.doi.org/10.2147/OTT.S287814 Text en © 2020 Duan et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Duan, Jiale
Yang, Yanping
Yin, Lei
Zhang, Xue
Tang, Yi
Zhang, Shuxian
Gong, Hanjuan
Xiao, Ming
Li, Ming
Li, Qingshu
Li, Xian
Yang, Lian
Fan, Qi
Wang, Yalan
Preliminary Study on the Identification of BRAF(V600E) Mutation in Colorectal Cancer by Near-Infrared Spectroscopy
title Preliminary Study on the Identification of BRAF(V600E) Mutation in Colorectal Cancer by Near-Infrared Spectroscopy
title_full Preliminary Study on the Identification of BRAF(V600E) Mutation in Colorectal Cancer by Near-Infrared Spectroscopy
title_fullStr Preliminary Study on the Identification of BRAF(V600E) Mutation in Colorectal Cancer by Near-Infrared Spectroscopy
title_full_unstemmed Preliminary Study on the Identification of BRAF(V600E) Mutation in Colorectal Cancer by Near-Infrared Spectroscopy
title_short Preliminary Study on the Identification of BRAF(V600E) Mutation in Colorectal Cancer by Near-Infrared Spectroscopy
title_sort preliminary study on the identification of braf(v600e) mutation in colorectal cancer by near-infrared spectroscopy
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7764696/
https://www.ncbi.nlm.nih.gov/pubmed/33376356
http://dx.doi.org/10.2147/OTT.S287814
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