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Improving Stability of Tear Film Lipid Layer via Concerted Action of Two Drug Molecules: A Biophysical View
The tear film at the ocular surface is covered by a thin layer of lipids. This oily phase stabilizes the film by decreasing its surface tension and improving its viscoelastic properties. Clinically, destabilization and rupture of the tear film are related to dry eye disease and are accompanied by ch...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7764870/ https://www.ncbi.nlm.nih.gov/pubmed/33327408 http://dx.doi.org/10.3390/ijms21249490 |
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author | Eftimov, Petar Olżyńska, Agnieszka Melcrová, Adéla Georgiev, Georgi As. Daull, Philippe Garrigue, Jean-Sebastien Cwiklik, Lukasz |
author_facet | Eftimov, Petar Olżyńska, Agnieszka Melcrová, Adéla Georgiev, Georgi As. Daull, Philippe Garrigue, Jean-Sebastien Cwiklik, Lukasz |
author_sort | Eftimov, Petar |
collection | PubMed |
description | The tear film at the ocular surface is covered by a thin layer of lipids. This oily phase stabilizes the film by decreasing its surface tension and improving its viscoelastic properties. Clinically, destabilization and rupture of the tear film are related to dry eye disease and are accompanied by changes in the quality and quantity of tear film lipids. In dry eye, eye drops containing oil-in-water emulsions are used for the supplementation of lipids and surface-active components to the tear film. We explore in detail the biophysical aspects of interactions of specific surface-active compounds, cetalkonium chloride and poloxamer 188, which are present in oil-in-water emulsions, with tear lipids. The aim is to better understand the macroscopically observed eye drops–tear film interactions by rationalizing them at the molecular level. To this end, we employ a multi-scale approach combining experiments on human meibomian lipid extracts, measurements using synthetic lipid films, and in silico molecular dynamics simulations. By combining these methods, we demonstrate that the studied compounds specifically interact with the tear lipid film enhancing its structure, surfactant properties, and elasticity. The observed effects are cooperative and can be further modulated by material packing at the tear–air interface. |
format | Online Article Text |
id | pubmed-7764870 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77648702020-12-27 Improving Stability of Tear Film Lipid Layer via Concerted Action of Two Drug Molecules: A Biophysical View Eftimov, Petar Olżyńska, Agnieszka Melcrová, Adéla Georgiev, Georgi As. Daull, Philippe Garrigue, Jean-Sebastien Cwiklik, Lukasz Int J Mol Sci Article The tear film at the ocular surface is covered by a thin layer of lipids. This oily phase stabilizes the film by decreasing its surface tension and improving its viscoelastic properties. Clinically, destabilization and rupture of the tear film are related to dry eye disease and are accompanied by changes in the quality and quantity of tear film lipids. In dry eye, eye drops containing oil-in-water emulsions are used for the supplementation of lipids and surface-active components to the tear film. We explore in detail the biophysical aspects of interactions of specific surface-active compounds, cetalkonium chloride and poloxamer 188, which are present in oil-in-water emulsions, with tear lipids. The aim is to better understand the macroscopically observed eye drops–tear film interactions by rationalizing them at the molecular level. To this end, we employ a multi-scale approach combining experiments on human meibomian lipid extracts, measurements using synthetic lipid films, and in silico molecular dynamics simulations. By combining these methods, we demonstrate that the studied compounds specifically interact with the tear lipid film enhancing its structure, surfactant properties, and elasticity. The observed effects are cooperative and can be further modulated by material packing at the tear–air interface. MDPI 2020-12-14 /pmc/articles/PMC7764870/ /pubmed/33327408 http://dx.doi.org/10.3390/ijms21249490 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Eftimov, Petar Olżyńska, Agnieszka Melcrová, Adéla Georgiev, Georgi As. Daull, Philippe Garrigue, Jean-Sebastien Cwiklik, Lukasz Improving Stability of Tear Film Lipid Layer via Concerted Action of Two Drug Molecules: A Biophysical View |
title | Improving Stability of Tear Film Lipid Layer via Concerted Action of Two Drug Molecules: A Biophysical View |
title_full | Improving Stability of Tear Film Lipid Layer via Concerted Action of Two Drug Molecules: A Biophysical View |
title_fullStr | Improving Stability of Tear Film Lipid Layer via Concerted Action of Two Drug Molecules: A Biophysical View |
title_full_unstemmed | Improving Stability of Tear Film Lipid Layer via Concerted Action of Two Drug Molecules: A Biophysical View |
title_short | Improving Stability of Tear Film Lipid Layer via Concerted Action of Two Drug Molecules: A Biophysical View |
title_sort | improving stability of tear film lipid layer via concerted action of two drug molecules: a biophysical view |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7764870/ https://www.ncbi.nlm.nih.gov/pubmed/33327408 http://dx.doi.org/10.3390/ijms21249490 |
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