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Improving Stability of Tear Film Lipid Layer via Concerted Action of Two Drug Molecules: A Biophysical View

The tear film at the ocular surface is covered by a thin layer of lipids. This oily phase stabilizes the film by decreasing its surface tension and improving its viscoelastic properties. Clinically, destabilization and rupture of the tear film are related to dry eye disease and are accompanied by ch...

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Autores principales: Eftimov, Petar, Olżyńska, Agnieszka, Melcrová, Adéla, Georgiev, Georgi As., Daull, Philippe, Garrigue, Jean-Sebastien, Cwiklik, Lukasz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7764870/
https://www.ncbi.nlm.nih.gov/pubmed/33327408
http://dx.doi.org/10.3390/ijms21249490
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author Eftimov, Petar
Olżyńska, Agnieszka
Melcrová, Adéla
Georgiev, Georgi As.
Daull, Philippe
Garrigue, Jean-Sebastien
Cwiklik, Lukasz
author_facet Eftimov, Petar
Olżyńska, Agnieszka
Melcrová, Adéla
Georgiev, Georgi As.
Daull, Philippe
Garrigue, Jean-Sebastien
Cwiklik, Lukasz
author_sort Eftimov, Petar
collection PubMed
description The tear film at the ocular surface is covered by a thin layer of lipids. This oily phase stabilizes the film by decreasing its surface tension and improving its viscoelastic properties. Clinically, destabilization and rupture of the tear film are related to dry eye disease and are accompanied by changes in the quality and quantity of tear film lipids. In dry eye, eye drops containing oil-in-water emulsions are used for the supplementation of lipids and surface-active components to the tear film. We explore in detail the biophysical aspects of interactions of specific surface-active compounds, cetalkonium chloride and poloxamer 188, which are present in oil-in-water emulsions, with tear lipids. The aim is to better understand the macroscopically observed eye drops–tear film interactions by rationalizing them at the molecular level. To this end, we employ a multi-scale approach combining experiments on human meibomian lipid extracts, measurements using synthetic lipid films, and in silico molecular dynamics simulations. By combining these methods, we demonstrate that the studied compounds specifically interact with the tear lipid film enhancing its structure, surfactant properties, and elasticity. The observed effects are cooperative and can be further modulated by material packing at the tear–air interface.
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spelling pubmed-77648702020-12-27 Improving Stability of Tear Film Lipid Layer via Concerted Action of Two Drug Molecules: A Biophysical View Eftimov, Petar Olżyńska, Agnieszka Melcrová, Adéla Georgiev, Georgi As. Daull, Philippe Garrigue, Jean-Sebastien Cwiklik, Lukasz Int J Mol Sci Article The tear film at the ocular surface is covered by a thin layer of lipids. This oily phase stabilizes the film by decreasing its surface tension and improving its viscoelastic properties. Clinically, destabilization and rupture of the tear film are related to dry eye disease and are accompanied by changes in the quality and quantity of tear film lipids. In dry eye, eye drops containing oil-in-water emulsions are used for the supplementation of lipids and surface-active components to the tear film. We explore in detail the biophysical aspects of interactions of specific surface-active compounds, cetalkonium chloride and poloxamer 188, which are present in oil-in-water emulsions, with tear lipids. The aim is to better understand the macroscopically observed eye drops–tear film interactions by rationalizing them at the molecular level. To this end, we employ a multi-scale approach combining experiments on human meibomian lipid extracts, measurements using synthetic lipid films, and in silico molecular dynamics simulations. By combining these methods, we demonstrate that the studied compounds specifically interact with the tear lipid film enhancing its structure, surfactant properties, and elasticity. The observed effects are cooperative and can be further modulated by material packing at the tear–air interface. MDPI 2020-12-14 /pmc/articles/PMC7764870/ /pubmed/33327408 http://dx.doi.org/10.3390/ijms21249490 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Eftimov, Petar
Olżyńska, Agnieszka
Melcrová, Adéla
Georgiev, Georgi As.
Daull, Philippe
Garrigue, Jean-Sebastien
Cwiklik, Lukasz
Improving Stability of Tear Film Lipid Layer via Concerted Action of Two Drug Molecules: A Biophysical View
title Improving Stability of Tear Film Lipid Layer via Concerted Action of Two Drug Molecules: A Biophysical View
title_full Improving Stability of Tear Film Lipid Layer via Concerted Action of Two Drug Molecules: A Biophysical View
title_fullStr Improving Stability of Tear Film Lipid Layer via Concerted Action of Two Drug Molecules: A Biophysical View
title_full_unstemmed Improving Stability of Tear Film Lipid Layer via Concerted Action of Two Drug Molecules: A Biophysical View
title_short Improving Stability of Tear Film Lipid Layer via Concerted Action of Two Drug Molecules: A Biophysical View
title_sort improving stability of tear film lipid layer via concerted action of two drug molecules: a biophysical view
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7764870/
https://www.ncbi.nlm.nih.gov/pubmed/33327408
http://dx.doi.org/10.3390/ijms21249490
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