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Low Repair Capacity of DNA Double-Strand Breaks Induced by Laser-Driven Ultrashort Electron Beams in Cancer Cells

Laser-driven accelerators allow to generate ultrashort (from femto- to picoseconds) high peak dose-rate (up to tens of GGy/s) accelerated particle beams. However, the radiobiological effects of ultrashort pulsed irradiation are still poorly studied. The aim of this work was to compare the formation...

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Autores principales: Babayan, Nelly, Vorobyeva, Natalia, Grigoryan, Bagrat, Grekhova, Anna, Pustovalova, Margarita, Rodneva, Sofya, Fedotov, Yuriy, Tsakanova, Gohar, Aroutiounian, Rouben, Osipov, Andreyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7764904/
https://www.ncbi.nlm.nih.gov/pubmed/33327380
http://dx.doi.org/10.3390/ijms21249488
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author Babayan, Nelly
Vorobyeva, Natalia
Grigoryan, Bagrat
Grekhova, Anna
Pustovalova, Margarita
Rodneva, Sofya
Fedotov, Yuriy
Tsakanova, Gohar
Aroutiounian, Rouben
Osipov, Andreyan
author_facet Babayan, Nelly
Vorobyeva, Natalia
Grigoryan, Bagrat
Grekhova, Anna
Pustovalova, Margarita
Rodneva, Sofya
Fedotov, Yuriy
Tsakanova, Gohar
Aroutiounian, Rouben
Osipov, Andreyan
author_sort Babayan, Nelly
collection PubMed
description Laser-driven accelerators allow to generate ultrashort (from femto- to picoseconds) high peak dose-rate (up to tens of GGy/s) accelerated particle beams. However, the radiobiological effects of ultrashort pulsed irradiation are still poorly studied. The aim of this work was to compare the formation and elimination of γH2AX and 53BP1 foci (well known markers for DNA double-strand breaks (DSBs)) in Hela cells exposed to ultrashort pulsed electron beams generated by Advanced Research Electron Accelerator Laboratory (AREAL) accelerator (electron energy 3.6 MeV, pulse duration 450 fs, pulse repetition rates 2 or 20 Hz) and quasi-continuous radiation generated by Varian accelerator (electron energy 4 MeV) at doses of 250–1000 mGy. Additionally, a study on the dose–response relationships of changes in the number of residual γH2AX foci in HeLa and A549 cells 24 h after irradiation at doses of 500–10,000 mGy were performed. We found no statistically significant differences in γH2AX and 53BP1 foci yields at 1 h after exposure to 2 Hz ultrashort pulse vs. quasi-continuous radiations. In contrast, 20 Hz ultrashort pulse irradiation resulted in 1.27-fold higher foci yields as compared to the quasi-continuous one. After 24 h of pulse irradiation at doses of 500–10,000 mGy the number of residual γH2AX foci in Hela and A549 cells was 1.7–2.9 times higher compared to that of quasi-continuous irradiation. Overall, the obtained results suggest the slower repair rate for DSBs induced by ultrashort pulse irradiation in comparison to DSBs induced by quasi-continuous irradiation.
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spelling pubmed-77649042020-12-27 Low Repair Capacity of DNA Double-Strand Breaks Induced by Laser-Driven Ultrashort Electron Beams in Cancer Cells Babayan, Nelly Vorobyeva, Natalia Grigoryan, Bagrat Grekhova, Anna Pustovalova, Margarita Rodneva, Sofya Fedotov, Yuriy Tsakanova, Gohar Aroutiounian, Rouben Osipov, Andreyan Int J Mol Sci Communication Laser-driven accelerators allow to generate ultrashort (from femto- to picoseconds) high peak dose-rate (up to tens of GGy/s) accelerated particle beams. However, the radiobiological effects of ultrashort pulsed irradiation are still poorly studied. The aim of this work was to compare the formation and elimination of γH2AX and 53BP1 foci (well known markers for DNA double-strand breaks (DSBs)) in Hela cells exposed to ultrashort pulsed electron beams generated by Advanced Research Electron Accelerator Laboratory (AREAL) accelerator (electron energy 3.6 MeV, pulse duration 450 fs, pulse repetition rates 2 or 20 Hz) and quasi-continuous radiation generated by Varian accelerator (electron energy 4 MeV) at doses of 250–1000 mGy. Additionally, a study on the dose–response relationships of changes in the number of residual γH2AX foci in HeLa and A549 cells 24 h after irradiation at doses of 500–10,000 mGy were performed. We found no statistically significant differences in γH2AX and 53BP1 foci yields at 1 h after exposure to 2 Hz ultrashort pulse vs. quasi-continuous radiations. In contrast, 20 Hz ultrashort pulse irradiation resulted in 1.27-fold higher foci yields as compared to the quasi-continuous one. After 24 h of pulse irradiation at doses of 500–10,000 mGy the number of residual γH2AX foci in Hela and A549 cells was 1.7–2.9 times higher compared to that of quasi-continuous irradiation. Overall, the obtained results suggest the slower repair rate for DSBs induced by ultrashort pulse irradiation in comparison to DSBs induced by quasi-continuous irradiation. MDPI 2020-12-14 /pmc/articles/PMC7764904/ /pubmed/33327380 http://dx.doi.org/10.3390/ijms21249488 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Babayan, Nelly
Vorobyeva, Natalia
Grigoryan, Bagrat
Grekhova, Anna
Pustovalova, Margarita
Rodneva, Sofya
Fedotov, Yuriy
Tsakanova, Gohar
Aroutiounian, Rouben
Osipov, Andreyan
Low Repair Capacity of DNA Double-Strand Breaks Induced by Laser-Driven Ultrashort Electron Beams in Cancer Cells
title Low Repair Capacity of DNA Double-Strand Breaks Induced by Laser-Driven Ultrashort Electron Beams in Cancer Cells
title_full Low Repair Capacity of DNA Double-Strand Breaks Induced by Laser-Driven Ultrashort Electron Beams in Cancer Cells
title_fullStr Low Repair Capacity of DNA Double-Strand Breaks Induced by Laser-Driven Ultrashort Electron Beams in Cancer Cells
title_full_unstemmed Low Repair Capacity of DNA Double-Strand Breaks Induced by Laser-Driven Ultrashort Electron Beams in Cancer Cells
title_short Low Repair Capacity of DNA Double-Strand Breaks Induced by Laser-Driven Ultrashort Electron Beams in Cancer Cells
title_sort low repair capacity of dna double-strand breaks induced by laser-driven ultrashort electron beams in cancer cells
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7764904/
https://www.ncbi.nlm.nih.gov/pubmed/33327380
http://dx.doi.org/10.3390/ijms21249488
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