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Clinical Molecular Epidemiology of Carbapenem-Resistant Klebsiella pneumoniae Among Pediatric Patients in Jiangsu Province, China

PURPOSE: The continuous emergence of carbapenem-resistant Klebsiella pneumoniae (CRKP) has become a serious public health problem globally, especially for children, but data on CRKP infection in pediatric patients are limited. This study aimed to identify epidemiological and molecular patterns of CR...

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Autores principales: Kong, Ziyan, Liu, Xuemei, Li, Chenxi, Cheng, Siyun, Xu, Fei, Gu, Bing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7764961/
https://www.ncbi.nlm.nih.gov/pubmed/33376368
http://dx.doi.org/10.2147/IDR.S293206
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author Kong, Ziyan
Liu, Xuemei
Li, Chenxi
Cheng, Siyun
Xu, Fei
Gu, Bing
author_facet Kong, Ziyan
Liu, Xuemei
Li, Chenxi
Cheng, Siyun
Xu, Fei
Gu, Bing
author_sort Kong, Ziyan
collection PubMed
description PURPOSE: The continuous emergence of carbapenem-resistant Klebsiella pneumoniae (CRKP) has become a serious public health problem globally, especially for children, but data on CRKP infection in pediatric patients are limited. This study aimed to identify epidemiological and molecular patterns of CRKP among pediatric patients in Jiangsu province, China. PATIENTS AND METHODS: CRKP were consecutively collected from the Children’s Hospital of Nanjing Medical University in China from July 2018 to May 2019. Then, CRKP strains were performed for further study: antimicrobial susceptibility testing, drug-resistance determinants screening and homology analysis. RESULTS: We collected 94 CRKP from 94 children. Overall, bla(KPC-2) (79.8%) was the predominant carbapenemase gene, followed by bla(NDM-1)(14.9%), bla(IMP-4) (5.3%) and bla(NDM-5)(4.3%). Notably, two isolates coharbored bla(KPC-2) and bla(IMP-4), and two isolates coharbored bla(KPC-2) and bla(NDM-5). MLST analysis revealed that 14 distinct sequence types (STs) were identified, of which ST11 was the most common sequence type identified. Moreover, two novel STs, ST4854 and ST4855, were detected in this study. PFGE revealed that a predominant cluster consisting of KPC-2-producing CRKP ST11 clone isolates was identified and was distributed mainly in the pediatric intensive care unit (PICU) and cardiac intensive care unit (CCU). Moreover, this is the first report to identify the dissemination of ST716 CRKP coproducing KPC-2 and IMP-4 clones. CONCLUSION: Clonal dissemination of KPC-2-producing CRKP ST11 was observed in multiple departments. Moreover, two novel STs (ST4854 and ST4855) were identified, which indicates an increased diversity of CRKP strains. To our knowledge, this is the first report that identified the dissemination of Klebsiella pneumoniae coproducing KPC-2 and IMP-4 clones among children, which represents a significant health risk to pediatric patients. Active surveillance and effective control measures are urgently needed to prevent further transmission of these strains among children.
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spelling pubmed-77649612020-12-28 Clinical Molecular Epidemiology of Carbapenem-Resistant Klebsiella pneumoniae Among Pediatric Patients in Jiangsu Province, China Kong, Ziyan Liu, Xuemei Li, Chenxi Cheng, Siyun Xu, Fei Gu, Bing Infect Drug Resist Original Research PURPOSE: The continuous emergence of carbapenem-resistant Klebsiella pneumoniae (CRKP) has become a serious public health problem globally, especially for children, but data on CRKP infection in pediatric patients are limited. This study aimed to identify epidemiological and molecular patterns of CRKP among pediatric patients in Jiangsu province, China. PATIENTS AND METHODS: CRKP were consecutively collected from the Children’s Hospital of Nanjing Medical University in China from July 2018 to May 2019. Then, CRKP strains were performed for further study: antimicrobial susceptibility testing, drug-resistance determinants screening and homology analysis. RESULTS: We collected 94 CRKP from 94 children. Overall, bla(KPC-2) (79.8%) was the predominant carbapenemase gene, followed by bla(NDM-1)(14.9%), bla(IMP-4) (5.3%) and bla(NDM-5)(4.3%). Notably, two isolates coharbored bla(KPC-2) and bla(IMP-4), and two isolates coharbored bla(KPC-2) and bla(NDM-5). MLST analysis revealed that 14 distinct sequence types (STs) were identified, of which ST11 was the most common sequence type identified. Moreover, two novel STs, ST4854 and ST4855, were detected in this study. PFGE revealed that a predominant cluster consisting of KPC-2-producing CRKP ST11 clone isolates was identified and was distributed mainly in the pediatric intensive care unit (PICU) and cardiac intensive care unit (CCU). Moreover, this is the first report to identify the dissemination of ST716 CRKP coproducing KPC-2 and IMP-4 clones. CONCLUSION: Clonal dissemination of KPC-2-producing CRKP ST11 was observed in multiple departments. Moreover, two novel STs (ST4854 and ST4855) were identified, which indicates an increased diversity of CRKP strains. To our knowledge, this is the first report that identified the dissemination of Klebsiella pneumoniae coproducing KPC-2 and IMP-4 clones among children, which represents a significant health risk to pediatric patients. Active surveillance and effective control measures are urgently needed to prevent further transmission of these strains among children. Dove 2020-12-22 /pmc/articles/PMC7764961/ /pubmed/33376368 http://dx.doi.org/10.2147/IDR.S293206 Text en © 2020 Kong et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Kong, Ziyan
Liu, Xuemei
Li, Chenxi
Cheng, Siyun
Xu, Fei
Gu, Bing
Clinical Molecular Epidemiology of Carbapenem-Resistant Klebsiella pneumoniae Among Pediatric Patients in Jiangsu Province, China
title Clinical Molecular Epidemiology of Carbapenem-Resistant Klebsiella pneumoniae Among Pediatric Patients in Jiangsu Province, China
title_full Clinical Molecular Epidemiology of Carbapenem-Resistant Klebsiella pneumoniae Among Pediatric Patients in Jiangsu Province, China
title_fullStr Clinical Molecular Epidemiology of Carbapenem-Resistant Klebsiella pneumoniae Among Pediatric Patients in Jiangsu Province, China
title_full_unstemmed Clinical Molecular Epidemiology of Carbapenem-Resistant Klebsiella pneumoniae Among Pediatric Patients in Jiangsu Province, China
title_short Clinical Molecular Epidemiology of Carbapenem-Resistant Klebsiella pneumoniae Among Pediatric Patients in Jiangsu Province, China
title_sort clinical molecular epidemiology of carbapenem-resistant klebsiella pneumoniae among pediatric patients in jiangsu province, china
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7764961/
https://www.ncbi.nlm.nih.gov/pubmed/33376368
http://dx.doi.org/10.2147/IDR.S293206
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