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Grape Seed Waste Counteracts Aflatoxin B1 Toxicity in Piglet Mesenteric Lymph Nodes

Aflatoxin B1 (AFB1) is a mycotoxin that frequently contaminates cereals and cereal byproducts. This study investigates the effect of AFB1 on the mesenteric lymph nodes (MLNs) of piglets and evaluates if a diet containing grape seed meal (GSM) can counteract the negative effect of AFB1 on inflammatio...

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Autores principales: Marin, Daniela Eliza, Bulgaru, Cristina Valeria, Anghel, Cristian Andrei, Pistol, Gina Cecilia, Dore, Madalina Ioana, Palade, Mihai Laurentiu, Taranu, Ionelia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7765275/
https://www.ncbi.nlm.nih.gov/pubmed/33333857
http://dx.doi.org/10.3390/toxins12120800
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author Marin, Daniela Eliza
Bulgaru, Cristina Valeria
Anghel, Cristian Andrei
Pistol, Gina Cecilia
Dore, Madalina Ioana
Palade, Mihai Laurentiu
Taranu, Ionelia
author_facet Marin, Daniela Eliza
Bulgaru, Cristina Valeria
Anghel, Cristian Andrei
Pistol, Gina Cecilia
Dore, Madalina Ioana
Palade, Mihai Laurentiu
Taranu, Ionelia
author_sort Marin, Daniela Eliza
collection PubMed
description Aflatoxin B1 (AFB1) is a mycotoxin that frequently contaminates cereals and cereal byproducts. This study investigates the effect of AFB1 on the mesenteric lymph nodes (MLNs) of piglets and evaluates if a diet containing grape seed meal (GSM) can counteract the negative effect of AFB1 on inflammation and oxidative stress. Twenty-four weaned piglets were fed the following diets: Control, AFB1 group (320 μg AFB1/kg feed), GSM group (8% GSM), and AFB1 + GSM group (8% GSM + 320 μg AFB1/kg feed) for 30 days. AFB1 has an important antioxidative effect by decreasing the activity of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) and total antioxidant status. As a result of the exposure to AFB1, an increase of MAP kinases, metalloproteinases, and cytokines, as effectors of an inflammatory response, were observed in the MLNs of intoxicated piglets. GSM induced a reduction of AFB1-induced oxidative stress by increasing the activity of GPx and SOD and by decreasing lipid peroxidation. GSM decreased the inflammatory markers increased by AFB1. These results represent an important and promising way to valorize this waste, which is rich in bioactive compounds, for decreasing AFB1 toxic effects in mesenteric lymph nodes.
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spelling pubmed-77652752020-12-27 Grape Seed Waste Counteracts Aflatoxin B1 Toxicity in Piglet Mesenteric Lymph Nodes Marin, Daniela Eliza Bulgaru, Cristina Valeria Anghel, Cristian Andrei Pistol, Gina Cecilia Dore, Madalina Ioana Palade, Mihai Laurentiu Taranu, Ionelia Toxins (Basel) Article Aflatoxin B1 (AFB1) is a mycotoxin that frequently contaminates cereals and cereal byproducts. This study investigates the effect of AFB1 on the mesenteric lymph nodes (MLNs) of piglets and evaluates if a diet containing grape seed meal (GSM) can counteract the negative effect of AFB1 on inflammation and oxidative stress. Twenty-four weaned piglets were fed the following diets: Control, AFB1 group (320 μg AFB1/kg feed), GSM group (8% GSM), and AFB1 + GSM group (8% GSM + 320 μg AFB1/kg feed) for 30 days. AFB1 has an important antioxidative effect by decreasing the activity of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) and total antioxidant status. As a result of the exposure to AFB1, an increase of MAP kinases, metalloproteinases, and cytokines, as effectors of an inflammatory response, were observed in the MLNs of intoxicated piglets. GSM induced a reduction of AFB1-induced oxidative stress by increasing the activity of GPx and SOD and by decreasing lipid peroxidation. GSM decreased the inflammatory markers increased by AFB1. These results represent an important and promising way to valorize this waste, which is rich in bioactive compounds, for decreasing AFB1 toxic effects in mesenteric lymph nodes. MDPI 2020-12-15 /pmc/articles/PMC7765275/ /pubmed/33333857 http://dx.doi.org/10.3390/toxins12120800 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Marin, Daniela Eliza
Bulgaru, Cristina Valeria
Anghel, Cristian Andrei
Pistol, Gina Cecilia
Dore, Madalina Ioana
Palade, Mihai Laurentiu
Taranu, Ionelia
Grape Seed Waste Counteracts Aflatoxin B1 Toxicity in Piglet Mesenteric Lymph Nodes
title Grape Seed Waste Counteracts Aflatoxin B1 Toxicity in Piglet Mesenteric Lymph Nodes
title_full Grape Seed Waste Counteracts Aflatoxin B1 Toxicity in Piglet Mesenteric Lymph Nodes
title_fullStr Grape Seed Waste Counteracts Aflatoxin B1 Toxicity in Piglet Mesenteric Lymph Nodes
title_full_unstemmed Grape Seed Waste Counteracts Aflatoxin B1 Toxicity in Piglet Mesenteric Lymph Nodes
title_short Grape Seed Waste Counteracts Aflatoxin B1 Toxicity in Piglet Mesenteric Lymph Nodes
title_sort grape seed waste counteracts aflatoxin b1 toxicity in piglet mesenteric lymph nodes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7765275/
https://www.ncbi.nlm.nih.gov/pubmed/33333857
http://dx.doi.org/10.3390/toxins12120800
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