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Three-Dimensional Human Cell Culture Models to Study the Pathophysiology of the Anterior Eye
In recent decades, the establishment of complex three-dimensional (3D) models of tissues has allowed researchers to perform high-quality studies and to not only advance knowledge of the physiology of these tissues but also mimic pathological conditions to test novel therapeutic strategies. The main...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7765302/ https://www.ncbi.nlm.nih.gov/pubmed/33333869 http://dx.doi.org/10.3390/pharmaceutics12121215 |
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author | García-Posadas, Laura Diebold, Yolanda |
author_facet | García-Posadas, Laura Diebold, Yolanda |
author_sort | García-Posadas, Laura |
collection | PubMed |
description | In recent decades, the establishment of complex three-dimensional (3D) models of tissues has allowed researchers to perform high-quality studies and to not only advance knowledge of the physiology of these tissues but also mimic pathological conditions to test novel therapeutic strategies. The main advantage of 3D models is that they recapitulate the spatial architecture of tissues and thereby provide more physiologically relevant information. The eye is an extremely complex organ that comprises a large variety of highly heterogeneous tissues that are divided into two asymmetrical portions: the anterior and posterior segments. The anterior segment consists of the cornea, conjunctiva, iris, ciliary body, sclera, aqueous humor, and the lens. Different diseases in these tissues can have devastating effects. To study these pathologies and develop new treatments, the use of cell culture models is instrumental, and the better the model, the more relevant the results. Thus, the development of sophisticated 3D models of ocular tissues is a significant challenge with enormous potential. In this review, we present a comprehensive overview of the latest advances in the development of 3D in vitro models of the anterior segment of the eye, with a special focus on those that use human primary cells. |
format | Online Article Text |
id | pubmed-7765302 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77653022020-12-27 Three-Dimensional Human Cell Culture Models to Study the Pathophysiology of the Anterior Eye García-Posadas, Laura Diebold, Yolanda Pharmaceutics Review In recent decades, the establishment of complex three-dimensional (3D) models of tissues has allowed researchers to perform high-quality studies and to not only advance knowledge of the physiology of these tissues but also mimic pathological conditions to test novel therapeutic strategies. The main advantage of 3D models is that they recapitulate the spatial architecture of tissues and thereby provide more physiologically relevant information. The eye is an extremely complex organ that comprises a large variety of highly heterogeneous tissues that are divided into two asymmetrical portions: the anterior and posterior segments. The anterior segment consists of the cornea, conjunctiva, iris, ciliary body, sclera, aqueous humor, and the lens. Different diseases in these tissues can have devastating effects. To study these pathologies and develop new treatments, the use of cell culture models is instrumental, and the better the model, the more relevant the results. Thus, the development of sophisticated 3D models of ocular tissues is a significant challenge with enormous potential. In this review, we present a comprehensive overview of the latest advances in the development of 3D in vitro models of the anterior segment of the eye, with a special focus on those that use human primary cells. MDPI 2020-12-15 /pmc/articles/PMC7765302/ /pubmed/33333869 http://dx.doi.org/10.3390/pharmaceutics12121215 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review García-Posadas, Laura Diebold, Yolanda Three-Dimensional Human Cell Culture Models to Study the Pathophysiology of the Anterior Eye |
title | Three-Dimensional Human Cell Culture Models to Study the Pathophysiology of the Anterior Eye |
title_full | Three-Dimensional Human Cell Culture Models to Study the Pathophysiology of the Anterior Eye |
title_fullStr | Three-Dimensional Human Cell Culture Models to Study the Pathophysiology of the Anterior Eye |
title_full_unstemmed | Three-Dimensional Human Cell Culture Models to Study the Pathophysiology of the Anterior Eye |
title_short | Three-Dimensional Human Cell Culture Models to Study the Pathophysiology of the Anterior Eye |
title_sort | three-dimensional human cell culture models to study the pathophysiology of the anterior eye |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7765302/ https://www.ncbi.nlm.nih.gov/pubmed/33333869 http://dx.doi.org/10.3390/pharmaceutics12121215 |
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