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Identification of a Candidate Starch Utilizing Strain of Prevotella albensis from Bovine Rumen

The inclusion of starch-rich feedstuffs, a common practice in intensive ruminant livestock production systems, can result in ruminal acidosis, a condition that can severely impact animal performance and health. One of the main causes of acidosis is the rapid accumulation of ruminal short chain fatty...

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Autores principales: Bandarupalli, Venkata Vinay Kumar, St-Pierre, Benoit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7765497/
https://www.ncbi.nlm.nih.gov/pubmed/33339094
http://dx.doi.org/10.3390/microorganisms8122005
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author Bandarupalli, Venkata Vinay Kumar
St-Pierre, Benoit
author_facet Bandarupalli, Venkata Vinay Kumar
St-Pierre, Benoit
author_sort Bandarupalli, Venkata Vinay Kumar
collection PubMed
description The inclusion of starch-rich feedstuffs, a common practice in intensive ruminant livestock production systems, can result in ruminal acidosis, a condition that can severely impact animal performance and health. One of the main causes of acidosis is the rapid accumulation of ruminal short chain fatty acids (SCFAs) resulting from the microbial digestion of starch. A greater understanding of ruminal bacterial amylolytic activities is therefore critical to improving mitigation of acidosis. To this end, our manuscript reports the identification of a candidate starch utilizer (OTU SD_Bt-00010) using batch culturing of bovine rumen fluid supplemented with starch. Based on 16S rRNA gene sequencing and metagenomics analysis, SD_Bt-00010 is predicted to be a currently uncharacterized strain of Prevotella albensis. Annotation of de novo assembled contigs from metagenomic data not only identified sequences encoding for α-amylase enzymes, but also revealed the potential to metabolize xylan as an alternative substrate. Metagenomics also predicted that SCFA end products for SD_Bt-00010 would be acetate and formate, and further suggested that this candidate strain may be a lactate utilizer. Together, these results indicate that SD_Bt-00010 is an amylolytic symbiont with beneficial attributes for its ruminant host.
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spelling pubmed-77654972020-12-27 Identification of a Candidate Starch Utilizing Strain of Prevotella albensis from Bovine Rumen Bandarupalli, Venkata Vinay Kumar St-Pierre, Benoit Microorganisms Article The inclusion of starch-rich feedstuffs, a common practice in intensive ruminant livestock production systems, can result in ruminal acidosis, a condition that can severely impact animal performance and health. One of the main causes of acidosis is the rapid accumulation of ruminal short chain fatty acids (SCFAs) resulting from the microbial digestion of starch. A greater understanding of ruminal bacterial amylolytic activities is therefore critical to improving mitigation of acidosis. To this end, our manuscript reports the identification of a candidate starch utilizer (OTU SD_Bt-00010) using batch culturing of bovine rumen fluid supplemented with starch. Based on 16S rRNA gene sequencing and metagenomics analysis, SD_Bt-00010 is predicted to be a currently uncharacterized strain of Prevotella albensis. Annotation of de novo assembled contigs from metagenomic data not only identified sequences encoding for α-amylase enzymes, but also revealed the potential to metabolize xylan as an alternative substrate. Metagenomics also predicted that SCFA end products for SD_Bt-00010 would be acetate and formate, and further suggested that this candidate strain may be a lactate utilizer. Together, these results indicate that SD_Bt-00010 is an amylolytic symbiont with beneficial attributes for its ruminant host. MDPI 2020-12-16 /pmc/articles/PMC7765497/ /pubmed/33339094 http://dx.doi.org/10.3390/microorganisms8122005 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bandarupalli, Venkata Vinay Kumar
St-Pierre, Benoit
Identification of a Candidate Starch Utilizing Strain of Prevotella albensis from Bovine Rumen
title Identification of a Candidate Starch Utilizing Strain of Prevotella albensis from Bovine Rumen
title_full Identification of a Candidate Starch Utilizing Strain of Prevotella albensis from Bovine Rumen
title_fullStr Identification of a Candidate Starch Utilizing Strain of Prevotella albensis from Bovine Rumen
title_full_unstemmed Identification of a Candidate Starch Utilizing Strain of Prevotella albensis from Bovine Rumen
title_short Identification of a Candidate Starch Utilizing Strain of Prevotella albensis from Bovine Rumen
title_sort identification of a candidate starch utilizing strain of prevotella albensis from bovine rumen
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7765497/
https://www.ncbi.nlm.nih.gov/pubmed/33339094
http://dx.doi.org/10.3390/microorganisms8122005
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