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Nerve Response to Superelastic Shape Memory Polyurethane Aerogels

We have previously shown the suitability of aerogels as scaffolds for neuronal cells. Here, we report on the use of superelastic shape memory polyurethane aerogels (SSMPA). SSMPA have a distinctly different stiffness than previously reported aerogels. The soft and deformable nature of SSMPA allowed...

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Autores principales: Sala, Martina Rodriguez, Skalli, Omar, Leventis, Nicholas, Sabri, Firouzeh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7765513/
https://www.ncbi.nlm.nih.gov/pubmed/33334083
http://dx.doi.org/10.3390/polym12122995
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author Sala, Martina Rodriguez
Skalli, Omar
Leventis, Nicholas
Sabri, Firouzeh
author_facet Sala, Martina Rodriguez
Skalli, Omar
Leventis, Nicholas
Sabri, Firouzeh
author_sort Sala, Martina Rodriguez
collection PubMed
description We have previously shown the suitability of aerogels as scaffolds for neuronal cells. Here, we report on the use of superelastic shape memory polyurethane aerogels (SSMPA). SSMPA have a distinctly different stiffness than previously reported aerogels. The soft and deformable nature of SSMPA allowed for radial compression of the aerogel induced by a custom designed apparatus. This radial compression changed the pore diameter and surface roughness (Sa) of SSMPA, while maintaining similar stiffness. Two varieties of SSMPA were used, Mix-14 and Mix-18, with distinctly different pore diameters and Sa. Radial compression led to a decreased pore diameter, which, in turn, decreased the Sa. The use of custom designed apparatus and two types of SSMPA allowed us to examine the influence of stiffness, pore size, and Sa on the extension of processes (neurites) by PC12 neuronal cells. PC12 cells plated on SSMPA with a higher degree of radial compression extended fewer neurites per cell when compared to other groups. However, the average length of the neurites was significantly longer when compared to the unrestricted group and to those extended by cells plated on SSMPA with less radial compression. These results demonstrate that SSMPA with 1.9 µm pore diameter, 1.17 µm Sa, and 203 kPa stiffness provides the optimum combination of physical parameters for nerve regeneration.
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spelling pubmed-77655132020-12-27 Nerve Response to Superelastic Shape Memory Polyurethane Aerogels Sala, Martina Rodriguez Skalli, Omar Leventis, Nicholas Sabri, Firouzeh Polymers (Basel) Article We have previously shown the suitability of aerogels as scaffolds for neuronal cells. Here, we report on the use of superelastic shape memory polyurethane aerogels (SSMPA). SSMPA have a distinctly different stiffness than previously reported aerogels. The soft and deformable nature of SSMPA allowed for radial compression of the aerogel induced by a custom designed apparatus. This radial compression changed the pore diameter and surface roughness (Sa) of SSMPA, while maintaining similar stiffness. Two varieties of SSMPA were used, Mix-14 and Mix-18, with distinctly different pore diameters and Sa. Radial compression led to a decreased pore diameter, which, in turn, decreased the Sa. The use of custom designed apparatus and two types of SSMPA allowed us to examine the influence of stiffness, pore size, and Sa on the extension of processes (neurites) by PC12 neuronal cells. PC12 cells plated on SSMPA with a higher degree of radial compression extended fewer neurites per cell when compared to other groups. However, the average length of the neurites was significantly longer when compared to the unrestricted group and to those extended by cells plated on SSMPA with less radial compression. These results demonstrate that SSMPA with 1.9 µm pore diameter, 1.17 µm Sa, and 203 kPa stiffness provides the optimum combination of physical parameters for nerve regeneration. MDPI 2020-12-15 /pmc/articles/PMC7765513/ /pubmed/33334083 http://dx.doi.org/10.3390/polym12122995 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sala, Martina Rodriguez
Skalli, Omar
Leventis, Nicholas
Sabri, Firouzeh
Nerve Response to Superelastic Shape Memory Polyurethane Aerogels
title Nerve Response to Superelastic Shape Memory Polyurethane Aerogels
title_full Nerve Response to Superelastic Shape Memory Polyurethane Aerogels
title_fullStr Nerve Response to Superelastic Shape Memory Polyurethane Aerogels
title_full_unstemmed Nerve Response to Superelastic Shape Memory Polyurethane Aerogels
title_short Nerve Response to Superelastic Shape Memory Polyurethane Aerogels
title_sort nerve response to superelastic shape memory polyurethane aerogels
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7765513/
https://www.ncbi.nlm.nih.gov/pubmed/33334083
http://dx.doi.org/10.3390/polym12122995
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