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Development of a Graphene-Based Biosensor for Detecting Recombinant Cyanovirin-N
We present a graphene-based biosensor selective to recombinant cyanovirin-N (rCV-N), an antiviral protein that has proven to be an effective microbicide to inhibit HIV replication. We modified the graphene monolayer devices with 1-pyrenebutanoic acid succinimidyl ester, which interacts with both gra...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7765543/ https://www.ncbi.nlm.nih.gov/pubmed/33339087 http://dx.doi.org/10.3390/bios10120206 |
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author | de Almeida, Pedro Rodrigues Murad, André Melro Silva, Luciano Paulino Rech, Elibio Leopoldo Alves, Elmo Salomão |
author_facet | de Almeida, Pedro Rodrigues Murad, André Melro Silva, Luciano Paulino Rech, Elibio Leopoldo Alves, Elmo Salomão |
author_sort | de Almeida, Pedro Rodrigues |
collection | PubMed |
description | We present a graphene-based biosensor selective to recombinant cyanovirin-N (rCV-N), an antiviral protein that has proven to be an effective microbicide to inhibit HIV replication. We modified the graphene monolayer devices with 1-pyrenebutanoic acid succinimidyl ester, which interacts with both graphene and the primary and secondary amines of antibodies. By monitoring the change in the electrical resistance of the device, we were able to detect rCV-N in solutions in the range of 0.01 to 10 ng/mL, and found that the detection limit was 0.45 pg/mL, which is much smaller than that obtained with currently available techniques. This is important for applications of this microbicide against HIV, since it may be produced at a large scale from soya bean seeds processed using the available industrial processing technologies. The sensor showed high sensitivity, selectivity, and reproducibility. |
format | Online Article Text |
id | pubmed-7765543 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77655432020-12-27 Development of a Graphene-Based Biosensor for Detecting Recombinant Cyanovirin-N de Almeida, Pedro Rodrigues Murad, André Melro Silva, Luciano Paulino Rech, Elibio Leopoldo Alves, Elmo Salomão Biosensors (Basel) Communication We present a graphene-based biosensor selective to recombinant cyanovirin-N (rCV-N), an antiviral protein that has proven to be an effective microbicide to inhibit HIV replication. We modified the graphene monolayer devices with 1-pyrenebutanoic acid succinimidyl ester, which interacts with both graphene and the primary and secondary amines of antibodies. By monitoring the change in the electrical resistance of the device, we were able to detect rCV-N in solutions in the range of 0.01 to 10 ng/mL, and found that the detection limit was 0.45 pg/mL, which is much smaller than that obtained with currently available techniques. This is important for applications of this microbicide against HIV, since it may be produced at a large scale from soya bean seeds processed using the available industrial processing technologies. The sensor showed high sensitivity, selectivity, and reproducibility. MDPI 2020-12-16 /pmc/articles/PMC7765543/ /pubmed/33339087 http://dx.doi.org/10.3390/bios10120206 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication de Almeida, Pedro Rodrigues Murad, André Melro Silva, Luciano Paulino Rech, Elibio Leopoldo Alves, Elmo Salomão Development of a Graphene-Based Biosensor for Detecting Recombinant Cyanovirin-N |
title | Development of a Graphene-Based Biosensor for Detecting Recombinant Cyanovirin-N |
title_full | Development of a Graphene-Based Biosensor for Detecting Recombinant Cyanovirin-N |
title_fullStr | Development of a Graphene-Based Biosensor for Detecting Recombinant Cyanovirin-N |
title_full_unstemmed | Development of a Graphene-Based Biosensor for Detecting Recombinant Cyanovirin-N |
title_short | Development of a Graphene-Based Biosensor for Detecting Recombinant Cyanovirin-N |
title_sort | development of a graphene-based biosensor for detecting recombinant cyanovirin-n |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7765543/ https://www.ncbi.nlm.nih.gov/pubmed/33339087 http://dx.doi.org/10.3390/bios10120206 |
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