Cargando…

Distinct African Swine Fever Virus Shedding in Wild Boar Infected with Virulent and Attenuated Isolates

Since the reappearance of African swine fever virus (ASFV), the disease has spread in an unprecedented animal pandemic in Eurasia. ASF currently constitutes the greatest global problem for the swine industry. The wild boar (Sus scrofa) in which the pathogen has established wild self-sustaining cycle...

Descripción completa

Detalles Bibliográficos
Autores principales: Kosowska, Aleksandra, Cadenas-Fernández, Estefanía, Barroso, Sandra, Sánchez-Vizcaíno, Jose M., Barasona, Jose A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7765575/
https://www.ncbi.nlm.nih.gov/pubmed/33339147
http://dx.doi.org/10.3390/vaccines8040767
_version_ 1783628522187653120
author Kosowska, Aleksandra
Cadenas-Fernández, Estefanía
Barroso, Sandra
Sánchez-Vizcaíno, Jose M.
Barasona, Jose A.
author_facet Kosowska, Aleksandra
Cadenas-Fernández, Estefanía
Barroso, Sandra
Sánchez-Vizcaíno, Jose M.
Barasona, Jose A.
author_sort Kosowska, Aleksandra
collection PubMed
description Since the reappearance of African swine fever virus (ASFV), the disease has spread in an unprecedented animal pandemic in Eurasia. ASF currently constitutes the greatest global problem for the swine industry. The wild boar (Sus scrofa) in which the pathogen has established wild self-sustaining cycles, is a key reservoir for ASFV, signifying that there is an urgent need to develop an effective vaccine against this virus. Current scientific debate addresses whether live attenuated vaccines (LAVs), which have shown promising results in cross-protection of susceptible hosts, may be feasible for vaccinations carried out owing to safety concerns. The objective of this study was, therefore, to compare the ASFV shedding in wild boar infected with virulent and attenuated (LAV) isolates. Different shedding routes (oral fluid and feces) and viremia rates were characterized in wild boar inoculated with Lv17/WB/Rie1 isolate (n = 12) when compared to those inoculated with the virulent Armenia07 isolate (n = 17). In general, fewer animals infected with the Lv17/WB/Rie1 isolate tested positive for ASFV in blood, oral fluid, and feces in comparison to animals infected with the virulent Armenia07 isolate. The shedding patterns were characterized in order to understand the transmission dynamics. This knowledge will help evaluate the shedding of new LAV candidates in wild boar populations, including the comparison with gene deletion mutant LAVs, whose current results are promising.
format Online
Article
Text
id pubmed-7765575
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-77655752020-12-27 Distinct African Swine Fever Virus Shedding in Wild Boar Infected with Virulent and Attenuated Isolates Kosowska, Aleksandra Cadenas-Fernández, Estefanía Barroso, Sandra Sánchez-Vizcaíno, Jose M. Barasona, Jose A. Vaccines (Basel) Article Since the reappearance of African swine fever virus (ASFV), the disease has spread in an unprecedented animal pandemic in Eurasia. ASF currently constitutes the greatest global problem for the swine industry. The wild boar (Sus scrofa) in which the pathogen has established wild self-sustaining cycles, is a key reservoir for ASFV, signifying that there is an urgent need to develop an effective vaccine against this virus. Current scientific debate addresses whether live attenuated vaccines (LAVs), which have shown promising results in cross-protection of susceptible hosts, may be feasible for vaccinations carried out owing to safety concerns. The objective of this study was, therefore, to compare the ASFV shedding in wild boar infected with virulent and attenuated (LAV) isolates. Different shedding routes (oral fluid and feces) and viremia rates were characterized in wild boar inoculated with Lv17/WB/Rie1 isolate (n = 12) when compared to those inoculated with the virulent Armenia07 isolate (n = 17). In general, fewer animals infected with the Lv17/WB/Rie1 isolate tested positive for ASFV in blood, oral fluid, and feces in comparison to animals infected with the virulent Armenia07 isolate. The shedding patterns were characterized in order to understand the transmission dynamics. This knowledge will help evaluate the shedding of new LAV candidates in wild boar populations, including the comparison with gene deletion mutant LAVs, whose current results are promising. MDPI 2020-12-16 /pmc/articles/PMC7765575/ /pubmed/33339147 http://dx.doi.org/10.3390/vaccines8040767 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kosowska, Aleksandra
Cadenas-Fernández, Estefanía
Barroso, Sandra
Sánchez-Vizcaíno, Jose M.
Barasona, Jose A.
Distinct African Swine Fever Virus Shedding in Wild Boar Infected with Virulent and Attenuated Isolates
title Distinct African Swine Fever Virus Shedding in Wild Boar Infected with Virulent and Attenuated Isolates
title_full Distinct African Swine Fever Virus Shedding in Wild Boar Infected with Virulent and Attenuated Isolates
title_fullStr Distinct African Swine Fever Virus Shedding in Wild Boar Infected with Virulent and Attenuated Isolates
title_full_unstemmed Distinct African Swine Fever Virus Shedding in Wild Boar Infected with Virulent and Attenuated Isolates
title_short Distinct African Swine Fever Virus Shedding in Wild Boar Infected with Virulent and Attenuated Isolates
title_sort distinct african swine fever virus shedding in wild boar infected with virulent and attenuated isolates
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7765575/
https://www.ncbi.nlm.nih.gov/pubmed/33339147
http://dx.doi.org/10.3390/vaccines8040767
work_keys_str_mv AT kosowskaaleksandra distinctafricanswinefevervirussheddinginwildboarinfectedwithvirulentandattenuatedisolates
AT cadenasfernandezestefania distinctafricanswinefevervirussheddinginwildboarinfectedwithvirulentandattenuatedisolates
AT barrososandra distinctafricanswinefevervirussheddinginwildboarinfectedwithvirulentandattenuatedisolates
AT sanchezvizcainojosem distinctafricanswinefevervirussheddinginwildboarinfectedwithvirulentandattenuatedisolates
AT barasonajosea distinctafricanswinefevervirussheddinginwildboarinfectedwithvirulentandattenuatedisolates