Cargando…
Fermented Soy-Derived Bioactive Peptides Selected by a Molecular Docking Approach Show Antioxidant Properties Involving the Keap1/Nrf2 Pathway
Bioactive peptides are a group of molecules with health beneficial properties, deriving from food matrices. They are protein fragments consisting of 2–20 amino acids that can be released by microbial fermentation, food processing and gastrointestinal digestion. Once hydrolyzed from their native prot...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7765838/ https://www.ncbi.nlm.nih.gov/pubmed/33352784 http://dx.doi.org/10.3390/antiox9121306 |
_version_ | 1783628575876841472 |
---|---|
author | Tonolo, Federica Moretto, Laura Grinzato, Alessandro Fiorese, Federico Folda, Alessandra Scalcon, Valeria Ferro, Stefania Arrigoni, Giorgio Bellamio, Marco Feller, Emiliano Bindoli, Alberto Marin, Oriano Rigobello, Maria Pia |
author_facet | Tonolo, Federica Moretto, Laura Grinzato, Alessandro Fiorese, Federico Folda, Alessandra Scalcon, Valeria Ferro, Stefania Arrigoni, Giorgio Bellamio, Marco Feller, Emiliano Bindoli, Alberto Marin, Oriano Rigobello, Maria Pia |
author_sort | Tonolo, Federica |
collection | PubMed |
description | Bioactive peptides are a group of molecules with health beneficial properties, deriving from food matrices. They are protein fragments consisting of 2–20 amino acids that can be released by microbial fermentation, food processing and gastrointestinal digestion. Once hydrolyzed from their native proteins, they can have different functions including antioxidant activity, which is important for cell protection by oxidant agents. In this work, fermented soy products were digested in vitro in order to improve the release of bioactive peptides. These were extracted, purified and analyzed in vitro and in a cellular model to assess their antioxidant activity. Peptide sequences were identified by LC-MS/MS analysis and a molecular docking approach was used to predict their ability to interact with Keap1, one of the key proteins of the Keap1/Nrf2 pathway, the major system involved in redox regulation. Peptides showing a high score of interaction were selected and tested for their antioxidant properties in a cellular environment using the Caco-2 cell line and examined for their capability to defend cells against oxidative stress. Our results indicate that several of the selected peptides were indeed able to activate the Keap1/Nrf2 pathway with the consequent overexpression of antioxidant and phase II enzymes. |
format | Online Article Text |
id | pubmed-7765838 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77658382020-12-28 Fermented Soy-Derived Bioactive Peptides Selected by a Molecular Docking Approach Show Antioxidant Properties Involving the Keap1/Nrf2 Pathway Tonolo, Federica Moretto, Laura Grinzato, Alessandro Fiorese, Federico Folda, Alessandra Scalcon, Valeria Ferro, Stefania Arrigoni, Giorgio Bellamio, Marco Feller, Emiliano Bindoli, Alberto Marin, Oriano Rigobello, Maria Pia Antioxidants (Basel) Article Bioactive peptides are a group of molecules with health beneficial properties, deriving from food matrices. They are protein fragments consisting of 2–20 amino acids that can be released by microbial fermentation, food processing and gastrointestinal digestion. Once hydrolyzed from their native proteins, they can have different functions including antioxidant activity, which is important for cell protection by oxidant agents. In this work, fermented soy products were digested in vitro in order to improve the release of bioactive peptides. These were extracted, purified and analyzed in vitro and in a cellular model to assess their antioxidant activity. Peptide sequences were identified by LC-MS/MS analysis and a molecular docking approach was used to predict their ability to interact with Keap1, one of the key proteins of the Keap1/Nrf2 pathway, the major system involved in redox regulation. Peptides showing a high score of interaction were selected and tested for their antioxidant properties in a cellular environment using the Caco-2 cell line and examined for their capability to defend cells against oxidative stress. Our results indicate that several of the selected peptides were indeed able to activate the Keap1/Nrf2 pathway with the consequent overexpression of antioxidant and phase II enzymes. MDPI 2020-12-19 /pmc/articles/PMC7765838/ /pubmed/33352784 http://dx.doi.org/10.3390/antiox9121306 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Tonolo, Federica Moretto, Laura Grinzato, Alessandro Fiorese, Federico Folda, Alessandra Scalcon, Valeria Ferro, Stefania Arrigoni, Giorgio Bellamio, Marco Feller, Emiliano Bindoli, Alberto Marin, Oriano Rigobello, Maria Pia Fermented Soy-Derived Bioactive Peptides Selected by a Molecular Docking Approach Show Antioxidant Properties Involving the Keap1/Nrf2 Pathway |
title | Fermented Soy-Derived Bioactive Peptides Selected by a Molecular Docking Approach Show Antioxidant Properties Involving the Keap1/Nrf2 Pathway |
title_full | Fermented Soy-Derived Bioactive Peptides Selected by a Molecular Docking Approach Show Antioxidant Properties Involving the Keap1/Nrf2 Pathway |
title_fullStr | Fermented Soy-Derived Bioactive Peptides Selected by a Molecular Docking Approach Show Antioxidant Properties Involving the Keap1/Nrf2 Pathway |
title_full_unstemmed | Fermented Soy-Derived Bioactive Peptides Selected by a Molecular Docking Approach Show Antioxidant Properties Involving the Keap1/Nrf2 Pathway |
title_short | Fermented Soy-Derived Bioactive Peptides Selected by a Molecular Docking Approach Show Antioxidant Properties Involving the Keap1/Nrf2 Pathway |
title_sort | fermented soy-derived bioactive peptides selected by a molecular docking approach show antioxidant properties involving the keap1/nrf2 pathway |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7765838/ https://www.ncbi.nlm.nih.gov/pubmed/33352784 http://dx.doi.org/10.3390/antiox9121306 |
work_keys_str_mv | AT tonolofederica fermentedsoyderivedbioactivepeptidesselectedbyamoleculardockingapproachshowantioxidantpropertiesinvolvingthekeap1nrf2pathway AT morettolaura fermentedsoyderivedbioactivepeptidesselectedbyamoleculardockingapproachshowantioxidantpropertiesinvolvingthekeap1nrf2pathway AT grinzatoalessandro fermentedsoyderivedbioactivepeptidesselectedbyamoleculardockingapproachshowantioxidantpropertiesinvolvingthekeap1nrf2pathway AT fioresefederico fermentedsoyderivedbioactivepeptidesselectedbyamoleculardockingapproachshowantioxidantpropertiesinvolvingthekeap1nrf2pathway AT foldaalessandra fermentedsoyderivedbioactivepeptidesselectedbyamoleculardockingapproachshowantioxidantpropertiesinvolvingthekeap1nrf2pathway AT scalconvaleria fermentedsoyderivedbioactivepeptidesselectedbyamoleculardockingapproachshowantioxidantpropertiesinvolvingthekeap1nrf2pathway AT ferrostefania fermentedsoyderivedbioactivepeptidesselectedbyamoleculardockingapproachshowantioxidantpropertiesinvolvingthekeap1nrf2pathway AT arrigonigiorgio fermentedsoyderivedbioactivepeptidesselectedbyamoleculardockingapproachshowantioxidantpropertiesinvolvingthekeap1nrf2pathway AT bellamiomarco fermentedsoyderivedbioactivepeptidesselectedbyamoleculardockingapproachshowantioxidantpropertiesinvolvingthekeap1nrf2pathway AT felleremiliano fermentedsoyderivedbioactivepeptidesselectedbyamoleculardockingapproachshowantioxidantpropertiesinvolvingthekeap1nrf2pathway AT bindolialberto fermentedsoyderivedbioactivepeptidesselectedbyamoleculardockingapproachshowantioxidantpropertiesinvolvingthekeap1nrf2pathway AT marinoriano fermentedsoyderivedbioactivepeptidesselectedbyamoleculardockingapproachshowantioxidantpropertiesinvolvingthekeap1nrf2pathway AT rigobellomariapia fermentedsoyderivedbioactivepeptidesselectedbyamoleculardockingapproachshowantioxidantpropertiesinvolvingthekeap1nrf2pathway |