Cargando…

Fermented Soy-Derived Bioactive Peptides Selected by a Molecular Docking Approach Show Antioxidant Properties Involving the Keap1/Nrf2 Pathway

Bioactive peptides are a group of molecules with health beneficial properties, deriving from food matrices. They are protein fragments consisting of 2–20 amino acids that can be released by microbial fermentation, food processing and gastrointestinal digestion. Once hydrolyzed from their native prot...

Descripción completa

Detalles Bibliográficos
Autores principales: Tonolo, Federica, Moretto, Laura, Grinzato, Alessandro, Fiorese, Federico, Folda, Alessandra, Scalcon, Valeria, Ferro, Stefania, Arrigoni, Giorgio, Bellamio, Marco, Feller, Emiliano, Bindoli, Alberto, Marin, Oriano, Rigobello, Maria Pia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7765838/
https://www.ncbi.nlm.nih.gov/pubmed/33352784
http://dx.doi.org/10.3390/antiox9121306
_version_ 1783628575876841472
author Tonolo, Federica
Moretto, Laura
Grinzato, Alessandro
Fiorese, Federico
Folda, Alessandra
Scalcon, Valeria
Ferro, Stefania
Arrigoni, Giorgio
Bellamio, Marco
Feller, Emiliano
Bindoli, Alberto
Marin, Oriano
Rigobello, Maria Pia
author_facet Tonolo, Federica
Moretto, Laura
Grinzato, Alessandro
Fiorese, Federico
Folda, Alessandra
Scalcon, Valeria
Ferro, Stefania
Arrigoni, Giorgio
Bellamio, Marco
Feller, Emiliano
Bindoli, Alberto
Marin, Oriano
Rigobello, Maria Pia
author_sort Tonolo, Federica
collection PubMed
description Bioactive peptides are a group of molecules with health beneficial properties, deriving from food matrices. They are protein fragments consisting of 2–20 amino acids that can be released by microbial fermentation, food processing and gastrointestinal digestion. Once hydrolyzed from their native proteins, they can have different functions including antioxidant activity, which is important for cell protection by oxidant agents. In this work, fermented soy products were digested in vitro in order to improve the release of bioactive peptides. These were extracted, purified and analyzed in vitro and in a cellular model to assess their antioxidant activity. Peptide sequences were identified by LC-MS/MS analysis and a molecular docking approach was used to predict their ability to interact with Keap1, one of the key proteins of the Keap1/Nrf2 pathway, the major system involved in redox regulation. Peptides showing a high score of interaction were selected and tested for their antioxidant properties in a cellular environment using the Caco-2 cell line and examined for their capability to defend cells against oxidative stress. Our results indicate that several of the selected peptides were indeed able to activate the Keap1/Nrf2 pathway with the consequent overexpression of antioxidant and phase II enzymes.
format Online
Article
Text
id pubmed-7765838
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-77658382020-12-28 Fermented Soy-Derived Bioactive Peptides Selected by a Molecular Docking Approach Show Antioxidant Properties Involving the Keap1/Nrf2 Pathway Tonolo, Federica Moretto, Laura Grinzato, Alessandro Fiorese, Federico Folda, Alessandra Scalcon, Valeria Ferro, Stefania Arrigoni, Giorgio Bellamio, Marco Feller, Emiliano Bindoli, Alberto Marin, Oriano Rigobello, Maria Pia Antioxidants (Basel) Article Bioactive peptides are a group of molecules with health beneficial properties, deriving from food matrices. They are protein fragments consisting of 2–20 amino acids that can be released by microbial fermentation, food processing and gastrointestinal digestion. Once hydrolyzed from their native proteins, they can have different functions including antioxidant activity, which is important for cell protection by oxidant agents. In this work, fermented soy products were digested in vitro in order to improve the release of bioactive peptides. These were extracted, purified and analyzed in vitro and in a cellular model to assess their antioxidant activity. Peptide sequences were identified by LC-MS/MS analysis and a molecular docking approach was used to predict their ability to interact with Keap1, one of the key proteins of the Keap1/Nrf2 pathway, the major system involved in redox regulation. Peptides showing a high score of interaction were selected and tested for their antioxidant properties in a cellular environment using the Caco-2 cell line and examined for their capability to defend cells against oxidative stress. Our results indicate that several of the selected peptides were indeed able to activate the Keap1/Nrf2 pathway with the consequent overexpression of antioxidant and phase II enzymes. MDPI 2020-12-19 /pmc/articles/PMC7765838/ /pubmed/33352784 http://dx.doi.org/10.3390/antiox9121306 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tonolo, Federica
Moretto, Laura
Grinzato, Alessandro
Fiorese, Federico
Folda, Alessandra
Scalcon, Valeria
Ferro, Stefania
Arrigoni, Giorgio
Bellamio, Marco
Feller, Emiliano
Bindoli, Alberto
Marin, Oriano
Rigobello, Maria Pia
Fermented Soy-Derived Bioactive Peptides Selected by a Molecular Docking Approach Show Antioxidant Properties Involving the Keap1/Nrf2 Pathway
title Fermented Soy-Derived Bioactive Peptides Selected by a Molecular Docking Approach Show Antioxidant Properties Involving the Keap1/Nrf2 Pathway
title_full Fermented Soy-Derived Bioactive Peptides Selected by a Molecular Docking Approach Show Antioxidant Properties Involving the Keap1/Nrf2 Pathway
title_fullStr Fermented Soy-Derived Bioactive Peptides Selected by a Molecular Docking Approach Show Antioxidant Properties Involving the Keap1/Nrf2 Pathway
title_full_unstemmed Fermented Soy-Derived Bioactive Peptides Selected by a Molecular Docking Approach Show Antioxidant Properties Involving the Keap1/Nrf2 Pathway
title_short Fermented Soy-Derived Bioactive Peptides Selected by a Molecular Docking Approach Show Antioxidant Properties Involving the Keap1/Nrf2 Pathway
title_sort fermented soy-derived bioactive peptides selected by a molecular docking approach show antioxidant properties involving the keap1/nrf2 pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7765838/
https://www.ncbi.nlm.nih.gov/pubmed/33352784
http://dx.doi.org/10.3390/antiox9121306
work_keys_str_mv AT tonolofederica fermentedsoyderivedbioactivepeptidesselectedbyamoleculardockingapproachshowantioxidantpropertiesinvolvingthekeap1nrf2pathway
AT morettolaura fermentedsoyderivedbioactivepeptidesselectedbyamoleculardockingapproachshowantioxidantpropertiesinvolvingthekeap1nrf2pathway
AT grinzatoalessandro fermentedsoyderivedbioactivepeptidesselectedbyamoleculardockingapproachshowantioxidantpropertiesinvolvingthekeap1nrf2pathway
AT fioresefederico fermentedsoyderivedbioactivepeptidesselectedbyamoleculardockingapproachshowantioxidantpropertiesinvolvingthekeap1nrf2pathway
AT foldaalessandra fermentedsoyderivedbioactivepeptidesselectedbyamoleculardockingapproachshowantioxidantpropertiesinvolvingthekeap1nrf2pathway
AT scalconvaleria fermentedsoyderivedbioactivepeptidesselectedbyamoleculardockingapproachshowantioxidantpropertiesinvolvingthekeap1nrf2pathway
AT ferrostefania fermentedsoyderivedbioactivepeptidesselectedbyamoleculardockingapproachshowantioxidantpropertiesinvolvingthekeap1nrf2pathway
AT arrigonigiorgio fermentedsoyderivedbioactivepeptidesselectedbyamoleculardockingapproachshowantioxidantpropertiesinvolvingthekeap1nrf2pathway
AT bellamiomarco fermentedsoyderivedbioactivepeptidesselectedbyamoleculardockingapproachshowantioxidantpropertiesinvolvingthekeap1nrf2pathway
AT felleremiliano fermentedsoyderivedbioactivepeptidesselectedbyamoleculardockingapproachshowantioxidantpropertiesinvolvingthekeap1nrf2pathway
AT bindolialberto fermentedsoyderivedbioactivepeptidesselectedbyamoleculardockingapproachshowantioxidantpropertiesinvolvingthekeap1nrf2pathway
AT marinoriano fermentedsoyderivedbioactivepeptidesselectedbyamoleculardockingapproachshowantioxidantpropertiesinvolvingthekeap1nrf2pathway
AT rigobellomariapia fermentedsoyderivedbioactivepeptidesselectedbyamoleculardockingapproachshowantioxidantpropertiesinvolvingthekeap1nrf2pathway