Cargando…

The Effect of Different Extraction Protocols on Brassica oleracea var. acephala Antioxidant Activity, Bioactive Compounds, and Sugar Profile

The extraction of glucosinolates in boiling aqueous methanol from freeze dried leaf tissues is the most common method for myrosinase inactivation but can be hazardous because of methanol toxicity. Although freeze drying is the best dehydration method in terms of nutritional quality preservation, the...

Descripción completa

Detalles Bibliográficos
Autores principales: Major, Nikola, Prekalj, Bernard, Perković, Josipa, Ban, Dean, Užila, Zoran, Ban, Smiljana Goreta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7766149/
https://www.ncbi.nlm.nih.gov/pubmed/33348742
http://dx.doi.org/10.3390/plants9121792
_version_ 1783628650025844736
author Major, Nikola
Prekalj, Bernard
Perković, Josipa
Ban, Dean
Užila, Zoran
Ban, Smiljana Goreta
author_facet Major, Nikola
Prekalj, Bernard
Perković, Josipa
Ban, Dean
Užila, Zoran
Ban, Smiljana Goreta
author_sort Major, Nikola
collection PubMed
description The extraction of glucosinolates in boiling aqueous methanol from freeze dried leaf tissues is the most common method for myrosinase inactivation but can be hazardous because of methanol toxicity. Although freeze drying is the best dehydration method in terms of nutritional quality preservation, the main drawbacks are a limited sample quantity that can be processed simultaneously, a long processing time, and high energy consumption. Therefore, the aim of this study is to evaluate the effects of applying high temperature for myrosinase inactivation via hot air drying prior to the extraction step, as well as the effects of cold aqueous methanol extraction on total antioxidant activity, total glucosinolates, total phenolic content, and sugar profile in 36 landraces of kale. The results from our study indicate that cold aqueous methanol can be used instead of boiling aqueous methanol with no adverse effects on total glucosinolate content. Our results also show that hot air drying, compared to freeze drying, followed by cold extraction has an adverse effect on antioxidant activity measured by DPPH radical scavenging, total glucosinolate content, as well as on the content of all investigated sugars.
format Online
Article
Text
id pubmed-7766149
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-77661492020-12-28 The Effect of Different Extraction Protocols on Brassica oleracea var. acephala Antioxidant Activity, Bioactive Compounds, and Sugar Profile Major, Nikola Prekalj, Bernard Perković, Josipa Ban, Dean Užila, Zoran Ban, Smiljana Goreta Plants (Basel) Article The extraction of glucosinolates in boiling aqueous methanol from freeze dried leaf tissues is the most common method for myrosinase inactivation but can be hazardous because of methanol toxicity. Although freeze drying is the best dehydration method in terms of nutritional quality preservation, the main drawbacks are a limited sample quantity that can be processed simultaneously, a long processing time, and high energy consumption. Therefore, the aim of this study is to evaluate the effects of applying high temperature for myrosinase inactivation via hot air drying prior to the extraction step, as well as the effects of cold aqueous methanol extraction on total antioxidant activity, total glucosinolates, total phenolic content, and sugar profile in 36 landraces of kale. The results from our study indicate that cold aqueous methanol can be used instead of boiling aqueous methanol with no adverse effects on total glucosinolate content. Our results also show that hot air drying, compared to freeze drying, followed by cold extraction has an adverse effect on antioxidant activity measured by DPPH radical scavenging, total glucosinolate content, as well as on the content of all investigated sugars. MDPI 2020-12-17 /pmc/articles/PMC7766149/ /pubmed/33348742 http://dx.doi.org/10.3390/plants9121792 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Major, Nikola
Prekalj, Bernard
Perković, Josipa
Ban, Dean
Užila, Zoran
Ban, Smiljana Goreta
The Effect of Different Extraction Protocols on Brassica oleracea var. acephala Antioxidant Activity, Bioactive Compounds, and Sugar Profile
title The Effect of Different Extraction Protocols on Brassica oleracea var. acephala Antioxidant Activity, Bioactive Compounds, and Sugar Profile
title_full The Effect of Different Extraction Protocols on Brassica oleracea var. acephala Antioxidant Activity, Bioactive Compounds, and Sugar Profile
title_fullStr The Effect of Different Extraction Protocols on Brassica oleracea var. acephala Antioxidant Activity, Bioactive Compounds, and Sugar Profile
title_full_unstemmed The Effect of Different Extraction Protocols on Brassica oleracea var. acephala Antioxidant Activity, Bioactive Compounds, and Sugar Profile
title_short The Effect of Different Extraction Protocols on Brassica oleracea var. acephala Antioxidant Activity, Bioactive Compounds, and Sugar Profile
title_sort effect of different extraction protocols on brassica oleracea var. acephala antioxidant activity, bioactive compounds, and sugar profile
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7766149/
https://www.ncbi.nlm.nih.gov/pubmed/33348742
http://dx.doi.org/10.3390/plants9121792
work_keys_str_mv AT majornikola theeffectofdifferentextractionprotocolsonbrassicaoleraceavaracephalaantioxidantactivitybioactivecompoundsandsugarprofile
AT prekaljbernard theeffectofdifferentextractionprotocolsonbrassicaoleraceavaracephalaantioxidantactivitybioactivecompoundsandsugarprofile
AT perkovicjosipa theeffectofdifferentextractionprotocolsonbrassicaoleraceavaracephalaantioxidantactivitybioactivecompoundsandsugarprofile
AT bandean theeffectofdifferentextractionprotocolsonbrassicaoleraceavaracephalaantioxidantactivitybioactivecompoundsandsugarprofile
AT uzilazoran theeffectofdifferentextractionprotocolsonbrassicaoleraceavaracephalaantioxidantactivitybioactivecompoundsandsugarprofile
AT bansmiljanagoreta theeffectofdifferentextractionprotocolsonbrassicaoleraceavaracephalaantioxidantactivitybioactivecompoundsandsugarprofile
AT majornikola effectofdifferentextractionprotocolsonbrassicaoleraceavaracephalaantioxidantactivitybioactivecompoundsandsugarprofile
AT prekaljbernard effectofdifferentextractionprotocolsonbrassicaoleraceavaracephalaantioxidantactivitybioactivecompoundsandsugarprofile
AT perkovicjosipa effectofdifferentextractionprotocolsonbrassicaoleraceavaracephalaantioxidantactivitybioactivecompoundsandsugarprofile
AT bandean effectofdifferentextractionprotocolsonbrassicaoleraceavaracephalaantioxidantactivitybioactivecompoundsandsugarprofile
AT uzilazoran effectofdifferentextractionprotocolsonbrassicaoleraceavaracephalaantioxidantactivitybioactivecompoundsandsugarprofile
AT bansmiljanagoreta effectofdifferentextractionprotocolsonbrassicaoleraceavaracephalaantioxidantactivitybioactivecompoundsandsugarprofile