Cargando…

Cell Penetrating Peptides Used in Delivery of Therapeutic Oligonucleotides Targeting Hepatitis B Virus

Peptide Nucleic Acid (PNAs) and small noncoding RNAs including small interfering RNAs (siRNAs) represent a new class of oligonucleotides considered as an alternative therapeutic strategy in the chronic hepatitis B treatment. Indeed, chronic hepatitis B virus (HBV) infection remains a major public he...

Descripción completa

Detalles Bibliográficos
Autores principales: Ndeboko, Bénédicte, Omouessi, Serge Thierry, Ongali, Brice, Mouinga-Ondémé, Augustin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7766285/
https://www.ncbi.nlm.nih.gov/pubmed/33371278
http://dx.doi.org/10.3390/ph13120483
_version_ 1783628682185670656
author Ndeboko, Bénédicte
Omouessi, Serge Thierry
Ongali, Brice
Mouinga-Ondémé, Augustin
author_facet Ndeboko, Bénédicte
Omouessi, Serge Thierry
Ongali, Brice
Mouinga-Ondémé, Augustin
author_sort Ndeboko, Bénédicte
collection PubMed
description Peptide Nucleic Acid (PNAs) and small noncoding RNAs including small interfering RNAs (siRNAs) represent a new class of oligonucleotides considered as an alternative therapeutic strategy in the chronic hepatitis B treatment. Indeed, chronic hepatitis B virus (HBV) infection remains a major public health problem worldwide, despite the availability of an effective prophylactic vaccine. Current therapeutic approaches approved for chronic HBV treatment are pegylated-interferon alpha (IFN)-α and nucleos(t)ide analogues (NAs). Both therapies do not completely eradicate viral infection and promote severe side effects. In this context, the development of new effective treatments is imperative. This review focuses on antiviral activity of both PNAs and siRNAs targeting hepatitis B virus. Thus, we briefly present our results on the ability of PNAs to decrease hepadnaviral replication in duck hepatitis B virus (DHBV) model. Interestingly, other oligonucleotides as siRNAs could significantly inhibit HBV antigen expression in transient replicative cell culture. Because the application of these oligonucleotides as new antiviral drugs has been hampered by their poor intracellular bioavailability, we also discuss the benefits of their coupling to different molecules such as the cell penetrating peptides (CPPs), which were used as vehicles to deliver therapeutic agents into the cells.
format Online
Article
Text
id pubmed-7766285
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-77662852020-12-28 Cell Penetrating Peptides Used in Delivery of Therapeutic Oligonucleotides Targeting Hepatitis B Virus Ndeboko, Bénédicte Omouessi, Serge Thierry Ongali, Brice Mouinga-Ondémé, Augustin Pharmaceuticals (Basel) Review Peptide Nucleic Acid (PNAs) and small noncoding RNAs including small interfering RNAs (siRNAs) represent a new class of oligonucleotides considered as an alternative therapeutic strategy in the chronic hepatitis B treatment. Indeed, chronic hepatitis B virus (HBV) infection remains a major public health problem worldwide, despite the availability of an effective prophylactic vaccine. Current therapeutic approaches approved for chronic HBV treatment are pegylated-interferon alpha (IFN)-α and nucleos(t)ide analogues (NAs). Both therapies do not completely eradicate viral infection and promote severe side effects. In this context, the development of new effective treatments is imperative. This review focuses on antiviral activity of both PNAs and siRNAs targeting hepatitis B virus. Thus, we briefly present our results on the ability of PNAs to decrease hepadnaviral replication in duck hepatitis B virus (DHBV) model. Interestingly, other oligonucleotides as siRNAs could significantly inhibit HBV antigen expression in transient replicative cell culture. Because the application of these oligonucleotides as new antiviral drugs has been hampered by their poor intracellular bioavailability, we also discuss the benefits of their coupling to different molecules such as the cell penetrating peptides (CPPs), which were used as vehicles to deliver therapeutic agents into the cells. MDPI 2020-12-21 /pmc/articles/PMC7766285/ /pubmed/33371278 http://dx.doi.org/10.3390/ph13120483 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Ndeboko, Bénédicte
Omouessi, Serge Thierry
Ongali, Brice
Mouinga-Ondémé, Augustin
Cell Penetrating Peptides Used in Delivery of Therapeutic Oligonucleotides Targeting Hepatitis B Virus
title Cell Penetrating Peptides Used in Delivery of Therapeutic Oligonucleotides Targeting Hepatitis B Virus
title_full Cell Penetrating Peptides Used in Delivery of Therapeutic Oligonucleotides Targeting Hepatitis B Virus
title_fullStr Cell Penetrating Peptides Used in Delivery of Therapeutic Oligonucleotides Targeting Hepatitis B Virus
title_full_unstemmed Cell Penetrating Peptides Used in Delivery of Therapeutic Oligonucleotides Targeting Hepatitis B Virus
title_short Cell Penetrating Peptides Used in Delivery of Therapeutic Oligonucleotides Targeting Hepatitis B Virus
title_sort cell penetrating peptides used in delivery of therapeutic oligonucleotides targeting hepatitis b virus
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7766285/
https://www.ncbi.nlm.nih.gov/pubmed/33371278
http://dx.doi.org/10.3390/ph13120483
work_keys_str_mv AT ndebokobenedicte cellpenetratingpeptidesusedindeliveryoftherapeuticoligonucleotidestargetinghepatitisbvirus
AT omouessisergethierry cellpenetratingpeptidesusedindeliveryoftherapeuticoligonucleotidestargetinghepatitisbvirus
AT ongalibrice cellpenetratingpeptidesusedindeliveryoftherapeuticoligonucleotidestargetinghepatitisbvirus
AT mouingaondemeaugustin cellpenetratingpeptidesusedindeliveryoftherapeuticoligonucleotidestargetinghepatitisbvirus