Cargando…

Heteroexpression of Aspergillus nidulans laeA in Marine-Derived Fungi Triggers Upregulation of Secondary Metabolite Biosynthetic Genes

Fungi are a prospective resource of bioactive compounds, but conventional methods of drug discovery are not effective enough to fully explore their metabolic potential. This study aimed to develop an easily attainable method to elicit the metabolic potential of fungi using Aspergillus nidulans laeA...

Descripción completa

Detalles Bibliográficos
Autores principales: Khan, Ishrat, Xie, Wan-Lin, Yu, Yu-Chao, Sheng, Huan, Xu, Yan, Wang, Jia-Qi, Debnath, Sanjit Chandra, Xu, Jin-Zhong, Zheng, Dao-Qiong, Ding, Wan-Jing, Wang, Pin-Mei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7766385/
https://www.ncbi.nlm.nih.gov/pubmed/33352941
http://dx.doi.org/10.3390/md18120652
_version_ 1783628706056503296
author Khan, Ishrat
Xie, Wan-Lin
Yu, Yu-Chao
Sheng, Huan
Xu, Yan
Wang, Jia-Qi
Debnath, Sanjit Chandra
Xu, Jin-Zhong
Zheng, Dao-Qiong
Ding, Wan-Jing
Wang, Pin-Mei
author_facet Khan, Ishrat
Xie, Wan-Lin
Yu, Yu-Chao
Sheng, Huan
Xu, Yan
Wang, Jia-Qi
Debnath, Sanjit Chandra
Xu, Jin-Zhong
Zheng, Dao-Qiong
Ding, Wan-Jing
Wang, Pin-Mei
author_sort Khan, Ishrat
collection PubMed
description Fungi are a prospective resource of bioactive compounds, but conventional methods of drug discovery are not effective enough to fully explore their metabolic potential. This study aimed to develop an easily attainable method to elicit the metabolic potential of fungi using Aspergillus nidulans laeA as a transcription regulation tool. In this study, functional analysis of Aspergillus nidulans laeA (AnLaeA) and Aspergillus sp. Z5 laeA (Az5LaeA) was done in the fungus Aspergillus sp. Z5. Heterologous AnLaeA-and native Az5LaeA-overexpression exhibited similar phenotypic effects and caused an increase in production of a bioactive compound diorcinol in Aspergillus sp. Z5, which proved the conserved function of this global regulator. In particular, heteroexpression of AnLaeA showed a significant impact on the expression of velvet complex genes, diorcinol synthesis-related genes, and different transcription factors (TFs). Moreover, heteroexpression of AnLaeA influenced the whole genome gene expression of Aspergillus sp. Z5 and triggered the upregulation of many genes. Overall, these findings suggest that heteroexpression of AnLaeA in fungi serves as a simple and easy method to explore their metabolic potential. In relation to this, AnLaeA was overexpressed in the fungus Penicillium sp. LC1-4, which resulted in increased production of quinolactacin A.
format Online
Article
Text
id pubmed-7766385
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-77663852020-12-28 Heteroexpression of Aspergillus nidulans laeA in Marine-Derived Fungi Triggers Upregulation of Secondary Metabolite Biosynthetic Genes Khan, Ishrat Xie, Wan-Lin Yu, Yu-Chao Sheng, Huan Xu, Yan Wang, Jia-Qi Debnath, Sanjit Chandra Xu, Jin-Zhong Zheng, Dao-Qiong Ding, Wan-Jing Wang, Pin-Mei Mar Drugs Article Fungi are a prospective resource of bioactive compounds, but conventional methods of drug discovery are not effective enough to fully explore their metabolic potential. This study aimed to develop an easily attainable method to elicit the metabolic potential of fungi using Aspergillus nidulans laeA as a transcription regulation tool. In this study, functional analysis of Aspergillus nidulans laeA (AnLaeA) and Aspergillus sp. Z5 laeA (Az5LaeA) was done in the fungus Aspergillus sp. Z5. Heterologous AnLaeA-and native Az5LaeA-overexpression exhibited similar phenotypic effects and caused an increase in production of a bioactive compound diorcinol in Aspergillus sp. Z5, which proved the conserved function of this global regulator. In particular, heteroexpression of AnLaeA showed a significant impact on the expression of velvet complex genes, diorcinol synthesis-related genes, and different transcription factors (TFs). Moreover, heteroexpression of AnLaeA influenced the whole genome gene expression of Aspergillus sp. Z5 and triggered the upregulation of many genes. Overall, these findings suggest that heteroexpression of AnLaeA in fungi serves as a simple and easy method to explore their metabolic potential. In relation to this, AnLaeA was overexpressed in the fungus Penicillium sp. LC1-4, which resulted in increased production of quinolactacin A. MDPI 2020-12-18 /pmc/articles/PMC7766385/ /pubmed/33352941 http://dx.doi.org/10.3390/md18120652 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Khan, Ishrat
Xie, Wan-Lin
Yu, Yu-Chao
Sheng, Huan
Xu, Yan
Wang, Jia-Qi
Debnath, Sanjit Chandra
Xu, Jin-Zhong
Zheng, Dao-Qiong
Ding, Wan-Jing
Wang, Pin-Mei
Heteroexpression of Aspergillus nidulans laeA in Marine-Derived Fungi Triggers Upregulation of Secondary Metabolite Biosynthetic Genes
title Heteroexpression of Aspergillus nidulans laeA in Marine-Derived Fungi Triggers Upregulation of Secondary Metabolite Biosynthetic Genes
title_full Heteroexpression of Aspergillus nidulans laeA in Marine-Derived Fungi Triggers Upregulation of Secondary Metabolite Biosynthetic Genes
title_fullStr Heteroexpression of Aspergillus nidulans laeA in Marine-Derived Fungi Triggers Upregulation of Secondary Metabolite Biosynthetic Genes
title_full_unstemmed Heteroexpression of Aspergillus nidulans laeA in Marine-Derived Fungi Triggers Upregulation of Secondary Metabolite Biosynthetic Genes
title_short Heteroexpression of Aspergillus nidulans laeA in Marine-Derived Fungi Triggers Upregulation of Secondary Metabolite Biosynthetic Genes
title_sort heteroexpression of aspergillus nidulans laea in marine-derived fungi triggers upregulation of secondary metabolite biosynthetic genes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7766385/
https://www.ncbi.nlm.nih.gov/pubmed/33352941
http://dx.doi.org/10.3390/md18120652
work_keys_str_mv AT khanishrat heteroexpressionofaspergillusnidulanslaeainmarinederivedfungitriggersupregulationofsecondarymetabolitebiosyntheticgenes
AT xiewanlin heteroexpressionofaspergillusnidulanslaeainmarinederivedfungitriggersupregulationofsecondarymetabolitebiosyntheticgenes
AT yuyuchao heteroexpressionofaspergillusnidulanslaeainmarinederivedfungitriggersupregulationofsecondarymetabolitebiosyntheticgenes
AT shenghuan heteroexpressionofaspergillusnidulanslaeainmarinederivedfungitriggersupregulationofsecondarymetabolitebiosyntheticgenes
AT xuyan heteroexpressionofaspergillusnidulanslaeainmarinederivedfungitriggersupregulationofsecondarymetabolitebiosyntheticgenes
AT wangjiaqi heteroexpressionofaspergillusnidulanslaeainmarinederivedfungitriggersupregulationofsecondarymetabolitebiosyntheticgenes
AT debnathsanjitchandra heteroexpressionofaspergillusnidulanslaeainmarinederivedfungitriggersupregulationofsecondarymetabolitebiosyntheticgenes
AT xujinzhong heteroexpressionofaspergillusnidulanslaeainmarinederivedfungitriggersupregulationofsecondarymetabolitebiosyntheticgenes
AT zhengdaoqiong heteroexpressionofaspergillusnidulanslaeainmarinederivedfungitriggersupregulationofsecondarymetabolitebiosyntheticgenes
AT dingwanjing heteroexpressionofaspergillusnidulanslaeainmarinederivedfungitriggersupregulationofsecondarymetabolitebiosyntheticgenes
AT wangpinmei heteroexpressionofaspergillusnidulanslaeainmarinederivedfungitriggersupregulationofsecondarymetabolitebiosyntheticgenes