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Prediction of Conserved Peptides of Paracoccidioides for Interferon-γ Release Assay: The First Step in the Development of a Lab-Based Approach for Immunological Assessment during Antifungal Therapy

Impaired antigen-specific cell-mediated immunity (CMI) is a primary immunological disturbance observed in individuals that develop paracoccidioidomycosis (PCM) after exposure to Paracoccidioides spp. Restoration of Paracoccidioides-specific CMI is crucial to stop the antifungal treatment and avoid r...

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Autores principales: Rosa, Sarah Brena Aparecida, Csordas, Bárbara Guimarães, do Valle Leone de Oliveira, Sandra Maria, Ribeiro dos Santos, Amanda, Paniago, Anamaria Mello Miranda, Venturini, James
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7766394/
https://www.ncbi.nlm.nih.gov/pubmed/33352628
http://dx.doi.org/10.3390/jof6040379
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author Rosa, Sarah Brena Aparecida
Csordas, Bárbara Guimarães
do Valle Leone de Oliveira, Sandra Maria
Ribeiro dos Santos, Amanda
Paniago, Anamaria Mello Miranda
Venturini, James
author_facet Rosa, Sarah Brena Aparecida
Csordas, Bárbara Guimarães
do Valle Leone de Oliveira, Sandra Maria
Ribeiro dos Santos, Amanda
Paniago, Anamaria Mello Miranda
Venturini, James
author_sort Rosa, Sarah Brena Aparecida
collection PubMed
description Impaired antigen-specific cell-mediated immunity (CMI) is a primary immunological disturbance observed in individuals that develop paracoccidioidomycosis (PCM) after exposure to Paracoccidioides spp. Restoration of Paracoccidioides-specific CMI is crucial to stop the antifungal treatment and avoid relapses. A convenient and specific laboratory tool to assess antigen specific CMI is required for the appropriate clinical treatment of fungal infections, in order to decrease the time of antifungal therapy. We used an interferon-γ release assay strategy, used in the diagnosis of latent tuberculosis infection, to address our aims in this study. Information on proteins secreted by two well-studied representative strains—Paracoccidioides brasiliensis (Pb18) and P. lutzii (Pb-01)—were explored using PubMed or MEDLINE. From 26 publications, 252 proteins were identified, of which 203 were similar according to the Basic Local Alignment Search Tool. This enabled a selection of conserved peptides using the MEGA software. The SignalP-5.0, TMHMM, IEDB, NetMHC II, and IFNepitope algorithms were used to identify appropriate epitopes. In our study, we predicted antigenic epitopes of Paracoccidioides that could bind to MHC class II and induce IFN-γ secretion. These T cell epitopes can be used in the development of a laboratory tool to monitor the CMI of patients with PCM.
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spelling pubmed-77663942020-12-28 Prediction of Conserved Peptides of Paracoccidioides for Interferon-γ Release Assay: The First Step in the Development of a Lab-Based Approach for Immunological Assessment during Antifungal Therapy Rosa, Sarah Brena Aparecida Csordas, Bárbara Guimarães do Valle Leone de Oliveira, Sandra Maria Ribeiro dos Santos, Amanda Paniago, Anamaria Mello Miranda Venturini, James J Fungi (Basel) Article Impaired antigen-specific cell-mediated immunity (CMI) is a primary immunological disturbance observed in individuals that develop paracoccidioidomycosis (PCM) after exposure to Paracoccidioides spp. Restoration of Paracoccidioides-specific CMI is crucial to stop the antifungal treatment and avoid relapses. A convenient and specific laboratory tool to assess antigen specific CMI is required for the appropriate clinical treatment of fungal infections, in order to decrease the time of antifungal therapy. We used an interferon-γ release assay strategy, used in the diagnosis of latent tuberculosis infection, to address our aims in this study. Information on proteins secreted by two well-studied representative strains—Paracoccidioides brasiliensis (Pb18) and P. lutzii (Pb-01)—were explored using PubMed or MEDLINE. From 26 publications, 252 proteins were identified, of which 203 were similar according to the Basic Local Alignment Search Tool. This enabled a selection of conserved peptides using the MEGA software. The SignalP-5.0, TMHMM, IEDB, NetMHC II, and IFNepitope algorithms were used to identify appropriate epitopes. In our study, we predicted antigenic epitopes of Paracoccidioides that could bind to MHC class II and induce IFN-γ secretion. These T cell epitopes can be used in the development of a laboratory tool to monitor the CMI of patients with PCM. MDPI 2020-12-19 /pmc/articles/PMC7766394/ /pubmed/33352628 http://dx.doi.org/10.3390/jof6040379 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rosa, Sarah Brena Aparecida
Csordas, Bárbara Guimarães
do Valle Leone de Oliveira, Sandra Maria
Ribeiro dos Santos, Amanda
Paniago, Anamaria Mello Miranda
Venturini, James
Prediction of Conserved Peptides of Paracoccidioides for Interferon-γ Release Assay: The First Step in the Development of a Lab-Based Approach for Immunological Assessment during Antifungal Therapy
title Prediction of Conserved Peptides of Paracoccidioides for Interferon-γ Release Assay: The First Step in the Development of a Lab-Based Approach for Immunological Assessment during Antifungal Therapy
title_full Prediction of Conserved Peptides of Paracoccidioides for Interferon-γ Release Assay: The First Step in the Development of a Lab-Based Approach for Immunological Assessment during Antifungal Therapy
title_fullStr Prediction of Conserved Peptides of Paracoccidioides for Interferon-γ Release Assay: The First Step in the Development of a Lab-Based Approach for Immunological Assessment during Antifungal Therapy
title_full_unstemmed Prediction of Conserved Peptides of Paracoccidioides for Interferon-γ Release Assay: The First Step in the Development of a Lab-Based Approach for Immunological Assessment during Antifungal Therapy
title_short Prediction of Conserved Peptides of Paracoccidioides for Interferon-γ Release Assay: The First Step in the Development of a Lab-Based Approach for Immunological Assessment during Antifungal Therapy
title_sort prediction of conserved peptides of paracoccidioides for interferon-γ release assay: the first step in the development of a lab-based approach for immunological assessment during antifungal therapy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7766394/
https://www.ncbi.nlm.nih.gov/pubmed/33352628
http://dx.doi.org/10.3390/jof6040379
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