Cargando…

Comparative Analysis of the Transcriptome, Proteome, and miRNA Profile of Kupffer Cells and Monocytes

Macrophage populations in most mammalian organs consist of cells of different origin. Resident macrophages originate from erythromyeloid precursors of the yolk sac wall; maintenance of the numbers of such macrophages in postnatal ontogenesis is practically independent of bone marrow haematopoiesis....

Descripción completa

Detalles Bibliográficos
Autores principales: Elchaninov, Andrey, Lokhonina, Anastasia, Nikitina, Maria, Vishnyakova, Polina, Makarov, Andrey, Arutyunyan, Irina, Poltavets, Anastasiya, Kananykhina, Evgenia, Kovalchuk, Sergey, Karpulevich, Evgeny, Bolshakova, Galina, Sukhikh, Gennady, Fatkhudinov, Timur
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7766432/
https://www.ncbi.nlm.nih.gov/pubmed/33352881
http://dx.doi.org/10.3390/biomedicines8120627
_version_ 1783628717260537856
author Elchaninov, Andrey
Lokhonina, Anastasia
Nikitina, Maria
Vishnyakova, Polina
Makarov, Andrey
Arutyunyan, Irina
Poltavets, Anastasiya
Kananykhina, Evgenia
Kovalchuk, Sergey
Karpulevich, Evgeny
Bolshakova, Galina
Sukhikh, Gennady
Fatkhudinov, Timur
author_facet Elchaninov, Andrey
Lokhonina, Anastasia
Nikitina, Maria
Vishnyakova, Polina
Makarov, Andrey
Arutyunyan, Irina
Poltavets, Anastasiya
Kananykhina, Evgenia
Kovalchuk, Sergey
Karpulevich, Evgeny
Bolshakova, Galina
Sukhikh, Gennady
Fatkhudinov, Timur
author_sort Elchaninov, Andrey
collection PubMed
description Macrophage populations in most mammalian organs consist of cells of different origin. Resident macrophages originate from erythromyeloid precursors of the yolk sac wall; maintenance of the numbers of such macrophages in postnatal ontogenesis is practically independent of bone marrow haematopoiesis. The largest populations of the resident macrophages of embryonic origin are found in the central nervous system (microglia) and liver (Kupffer cells). In contrast, skin dermis and mucous membranes become predominantly colonized by bone marrow-derived monocytes that show pronounced functional and phenotypic plasticity. In the present study, we compared Kupffer cells and monocytes using the immunophenotype, gene expression profile, proteome, and pool of microRNA. The observed differences did not consider the resident liver macrophages as purely M2 macrophages or state that monocytes have purely M1 features. Monocytes show signs of high plasticity and sensitivity to pathogen-associated molecular patterns (e.g., high levels of transcription for Tlr 2, 4, 7, and 8). In contrast, the resident liver macrophages were clearly involved in the regulation of specific organ functions (nitrogen metabolism, complement system protein synthesis).
format Online
Article
Text
id pubmed-7766432
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-77664322020-12-28 Comparative Analysis of the Transcriptome, Proteome, and miRNA Profile of Kupffer Cells and Monocytes Elchaninov, Andrey Lokhonina, Anastasia Nikitina, Maria Vishnyakova, Polina Makarov, Andrey Arutyunyan, Irina Poltavets, Anastasiya Kananykhina, Evgenia Kovalchuk, Sergey Karpulevich, Evgeny Bolshakova, Galina Sukhikh, Gennady Fatkhudinov, Timur Biomedicines Article Macrophage populations in most mammalian organs consist of cells of different origin. Resident macrophages originate from erythromyeloid precursors of the yolk sac wall; maintenance of the numbers of such macrophages in postnatal ontogenesis is practically independent of bone marrow haematopoiesis. The largest populations of the resident macrophages of embryonic origin are found in the central nervous system (microglia) and liver (Kupffer cells). In contrast, skin dermis and mucous membranes become predominantly colonized by bone marrow-derived monocytes that show pronounced functional and phenotypic plasticity. In the present study, we compared Kupffer cells and monocytes using the immunophenotype, gene expression profile, proteome, and pool of microRNA. The observed differences did not consider the resident liver macrophages as purely M2 macrophages or state that monocytes have purely M1 features. Monocytes show signs of high plasticity and sensitivity to pathogen-associated molecular patterns (e.g., high levels of transcription for Tlr 2, 4, 7, and 8). In contrast, the resident liver macrophages were clearly involved in the regulation of specific organ functions (nitrogen metabolism, complement system protein synthesis). MDPI 2020-12-18 /pmc/articles/PMC7766432/ /pubmed/33352881 http://dx.doi.org/10.3390/biomedicines8120627 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Elchaninov, Andrey
Lokhonina, Anastasia
Nikitina, Maria
Vishnyakova, Polina
Makarov, Andrey
Arutyunyan, Irina
Poltavets, Anastasiya
Kananykhina, Evgenia
Kovalchuk, Sergey
Karpulevich, Evgeny
Bolshakova, Galina
Sukhikh, Gennady
Fatkhudinov, Timur
Comparative Analysis of the Transcriptome, Proteome, and miRNA Profile of Kupffer Cells and Monocytes
title Comparative Analysis of the Transcriptome, Proteome, and miRNA Profile of Kupffer Cells and Monocytes
title_full Comparative Analysis of the Transcriptome, Proteome, and miRNA Profile of Kupffer Cells and Monocytes
title_fullStr Comparative Analysis of the Transcriptome, Proteome, and miRNA Profile of Kupffer Cells and Monocytes
title_full_unstemmed Comparative Analysis of the Transcriptome, Proteome, and miRNA Profile of Kupffer Cells and Monocytes
title_short Comparative Analysis of the Transcriptome, Proteome, and miRNA Profile of Kupffer Cells and Monocytes
title_sort comparative analysis of the transcriptome, proteome, and mirna profile of kupffer cells and monocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7766432/
https://www.ncbi.nlm.nih.gov/pubmed/33352881
http://dx.doi.org/10.3390/biomedicines8120627
work_keys_str_mv AT elchaninovandrey comparativeanalysisofthetranscriptomeproteomeandmirnaprofileofkupffercellsandmonocytes
AT lokhoninaanastasia comparativeanalysisofthetranscriptomeproteomeandmirnaprofileofkupffercellsandmonocytes
AT nikitinamaria comparativeanalysisofthetranscriptomeproteomeandmirnaprofileofkupffercellsandmonocytes
AT vishnyakovapolina comparativeanalysisofthetranscriptomeproteomeandmirnaprofileofkupffercellsandmonocytes
AT makarovandrey comparativeanalysisofthetranscriptomeproteomeandmirnaprofileofkupffercellsandmonocytes
AT arutyunyanirina comparativeanalysisofthetranscriptomeproteomeandmirnaprofileofkupffercellsandmonocytes
AT poltavetsanastasiya comparativeanalysisofthetranscriptomeproteomeandmirnaprofileofkupffercellsandmonocytes
AT kananykhinaevgenia comparativeanalysisofthetranscriptomeproteomeandmirnaprofileofkupffercellsandmonocytes
AT kovalchuksergey comparativeanalysisofthetranscriptomeproteomeandmirnaprofileofkupffercellsandmonocytes
AT karpulevichevgeny comparativeanalysisofthetranscriptomeproteomeandmirnaprofileofkupffercellsandmonocytes
AT bolshakovagalina comparativeanalysisofthetranscriptomeproteomeandmirnaprofileofkupffercellsandmonocytes
AT sukhikhgennady comparativeanalysisofthetranscriptomeproteomeandmirnaprofileofkupffercellsandmonocytes
AT fatkhudinovtimur comparativeanalysisofthetranscriptomeproteomeandmirnaprofileofkupffercellsandmonocytes