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Genetic Diversity of Potyviruses Associated with Tulip Breaking Syndrome
Tulip breaking is economically the most important viral disease of modern-day tulip growing. It is characterized by irregular flame and feather-like patterns in the flowers and mosaic on the foliage. Thirty-two leaf samples were collected from cultivated tulip plants showing tulip breaking syndrome...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7766433/ https://www.ncbi.nlm.nih.gov/pubmed/33352796 http://dx.doi.org/10.3390/plants9121807 |
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author | Ágoston, János Almási, Asztéria Salánki, Katalin Palkovics, László |
author_facet | Ágoston, János Almási, Asztéria Salánki, Katalin Palkovics, László |
author_sort | Ágoston, János |
collection | PubMed |
description | Tulip breaking is economically the most important viral disease of modern-day tulip growing. It is characterized by irregular flame and feather-like patterns in the flowers and mosaic on the foliage. Thirty-two leaf samples were collected from cultivated tulip plants showing tulip breaking syndrome from Hungary in 2017 and 2018. Virus identification was performed by serological (ELISA) and molecular (RT-PCR) methods. All samples proved to be infected with a potyvirus and evidence was provided that three potyvirus species could be identified in the samples: Lily mottle virus (LMoV), Tulip breaking virus (TBV) and Rembrandt tulip-breaking virus (ReTBV). Recombination prediction accomplished with Recombination Detection Program (RDP) v4.98 revealed potential intraspecies recombination in the case of TBV and LMoV. Phylogenetic analyses of the coat protein (CP) regions proved the monophyletic origin of these viruses and verified them as three different species according to current International Committee on Taxonomy of Viruses (ICTV) species demarcation criteria. Based on these results, we analyzed taxonomic relations concerning potyviruses associated with tulip breaking syndrome. We propose the elevation of ReTBV to species level, and emergence of two new subgroups in ReTBV. |
format | Online Article Text |
id | pubmed-7766433 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77664332020-12-28 Genetic Diversity of Potyviruses Associated with Tulip Breaking Syndrome Ágoston, János Almási, Asztéria Salánki, Katalin Palkovics, László Plants (Basel) Article Tulip breaking is economically the most important viral disease of modern-day tulip growing. It is characterized by irregular flame and feather-like patterns in the flowers and mosaic on the foliage. Thirty-two leaf samples were collected from cultivated tulip plants showing tulip breaking syndrome from Hungary in 2017 and 2018. Virus identification was performed by serological (ELISA) and molecular (RT-PCR) methods. All samples proved to be infected with a potyvirus and evidence was provided that three potyvirus species could be identified in the samples: Lily mottle virus (LMoV), Tulip breaking virus (TBV) and Rembrandt tulip-breaking virus (ReTBV). Recombination prediction accomplished with Recombination Detection Program (RDP) v4.98 revealed potential intraspecies recombination in the case of TBV and LMoV. Phylogenetic analyses of the coat protein (CP) regions proved the monophyletic origin of these viruses and verified them as three different species according to current International Committee on Taxonomy of Viruses (ICTV) species demarcation criteria. Based on these results, we analyzed taxonomic relations concerning potyviruses associated with tulip breaking syndrome. We propose the elevation of ReTBV to species level, and emergence of two new subgroups in ReTBV. MDPI 2020-12-19 /pmc/articles/PMC7766433/ /pubmed/33352796 http://dx.doi.org/10.3390/plants9121807 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ágoston, János Almási, Asztéria Salánki, Katalin Palkovics, László Genetic Diversity of Potyviruses Associated with Tulip Breaking Syndrome |
title | Genetic Diversity of Potyviruses Associated with Tulip Breaking Syndrome |
title_full | Genetic Diversity of Potyviruses Associated with Tulip Breaking Syndrome |
title_fullStr | Genetic Diversity of Potyviruses Associated with Tulip Breaking Syndrome |
title_full_unstemmed | Genetic Diversity of Potyviruses Associated with Tulip Breaking Syndrome |
title_short | Genetic Diversity of Potyviruses Associated with Tulip Breaking Syndrome |
title_sort | genetic diversity of potyviruses associated with tulip breaking syndrome |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7766433/ https://www.ncbi.nlm.nih.gov/pubmed/33352796 http://dx.doi.org/10.3390/plants9121807 |
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