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Proteomic Analysis of the Venom of Jellyfishes Rhopilema esculentum and Sanderia malayensis

Venomics, the study of biological venoms, could potentially provide a new source of therapeutic compounds, yet information on the venoms from marine organisms, including cnidarians (sea anemones, corals, and jellyfish), is limited. This study identified the putative toxins of two species of jellyfis...

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Autores principales: Leung, Thomas C. N., Qu, Zhe, Nong, Wenyan, Hui, Jerome H. L., Ngai, Sai Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7766711/
https://www.ncbi.nlm.nih.gov/pubmed/33371176
http://dx.doi.org/10.3390/md18120655
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author Leung, Thomas C. N.
Qu, Zhe
Nong, Wenyan
Hui, Jerome H. L.
Ngai, Sai Ming
author_facet Leung, Thomas C. N.
Qu, Zhe
Nong, Wenyan
Hui, Jerome H. L.
Ngai, Sai Ming
author_sort Leung, Thomas C. N.
collection PubMed
description Venomics, the study of biological venoms, could potentially provide a new source of therapeutic compounds, yet information on the venoms from marine organisms, including cnidarians (sea anemones, corals, and jellyfish), is limited. This study identified the putative toxins of two species of jellyfish—edible jellyfish Rhopilema esculentum Kishinouye, 1891, also known as flame jellyfish, and Amuska jellyfish Sanderia malayensis Goette, 1886. Utilizing nano-flow liquid chromatography tandem mass spectrometry (nLC–MS/MS), 3000 proteins were identified from the nematocysts in each of the above two jellyfish species. Forty and fifty-one putative toxins were identified in R. esculentum and S. malayensis, respectively, which were further classified into eight toxin families according to their predicted functions. Amongst the identified putative toxins, hemostasis-impairing toxins and proteases were found to be the most dominant members (>60%). The present study demonstrates the first proteomes of nematocysts from two jellyfish species with economic and environmental importance, and expands the foundation and understanding of cnidarian toxins.
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spelling pubmed-77667112020-12-28 Proteomic Analysis of the Venom of Jellyfishes Rhopilema esculentum and Sanderia malayensis Leung, Thomas C. N. Qu, Zhe Nong, Wenyan Hui, Jerome H. L. Ngai, Sai Ming Mar Drugs Article Venomics, the study of biological venoms, could potentially provide a new source of therapeutic compounds, yet information on the venoms from marine organisms, including cnidarians (sea anemones, corals, and jellyfish), is limited. This study identified the putative toxins of two species of jellyfish—edible jellyfish Rhopilema esculentum Kishinouye, 1891, also known as flame jellyfish, and Amuska jellyfish Sanderia malayensis Goette, 1886. Utilizing nano-flow liquid chromatography tandem mass spectrometry (nLC–MS/MS), 3000 proteins were identified from the nematocysts in each of the above two jellyfish species. Forty and fifty-one putative toxins were identified in R. esculentum and S. malayensis, respectively, which were further classified into eight toxin families according to their predicted functions. Amongst the identified putative toxins, hemostasis-impairing toxins and proteases were found to be the most dominant members (>60%). The present study demonstrates the first proteomes of nematocysts from two jellyfish species with economic and environmental importance, and expands the foundation and understanding of cnidarian toxins. MDPI 2020-12-21 /pmc/articles/PMC7766711/ /pubmed/33371176 http://dx.doi.org/10.3390/md18120655 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Leung, Thomas C. N.
Qu, Zhe
Nong, Wenyan
Hui, Jerome H. L.
Ngai, Sai Ming
Proteomic Analysis of the Venom of Jellyfishes Rhopilema esculentum and Sanderia malayensis
title Proteomic Analysis of the Venom of Jellyfishes Rhopilema esculentum and Sanderia malayensis
title_full Proteomic Analysis of the Venom of Jellyfishes Rhopilema esculentum and Sanderia malayensis
title_fullStr Proteomic Analysis of the Venom of Jellyfishes Rhopilema esculentum and Sanderia malayensis
title_full_unstemmed Proteomic Analysis of the Venom of Jellyfishes Rhopilema esculentum and Sanderia malayensis
title_short Proteomic Analysis of the Venom of Jellyfishes Rhopilema esculentum and Sanderia malayensis
title_sort proteomic analysis of the venom of jellyfishes rhopilema esculentum and sanderia malayensis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7766711/
https://www.ncbi.nlm.nih.gov/pubmed/33371176
http://dx.doi.org/10.3390/md18120655
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