Discovery and Characterization of a Novel Ampelovirus on Firespike

A novel RNA virus was identified in firespike (Odontonema tubaeforme) plants exhibiting leaf curling and chlorosis. The molecular features of the viral genomic RNA and proteins resemble those of ampeloviruses. Based on sequence comparisons and phylogenetic analysis, we propose a new species in the g...

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Autores principales: Wang, Yaqin, Song, Yu, Wang, Yongzhi, Cao, Mengji, Hu, Tao, Zhou, Xueping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7766885/
https://www.ncbi.nlm.nih.gov/pubmed/33339381
http://dx.doi.org/10.3390/v12121452
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author Wang, Yaqin
Song, Yu
Wang, Yongzhi
Cao, Mengji
Hu, Tao
Zhou, Xueping
author_facet Wang, Yaqin
Song, Yu
Wang, Yongzhi
Cao, Mengji
Hu, Tao
Zhou, Xueping
author_sort Wang, Yaqin
collection PubMed
description A novel RNA virus was identified in firespike (Odontonema tubaeforme) plants exhibiting leaf curling and chlorosis. The molecular features of the viral genomic RNA and proteins resemble those of ampeloviruses. Based on sequence comparisons and phylogenetic analysis, we propose a new species in the genus Ampelovirus, which we have tentatively named Firespike leafroll-associated virus (FLRaV). Bioassays showed that the virus is mechanically transmissible to Nicotiana benthamiana. In addition, a full-length cDNA clone of FLRaV could successfully infect N. benthamiana via agroinfiltration.
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spelling pubmed-77668852020-12-28 Discovery and Characterization of a Novel Ampelovirus on Firespike Wang, Yaqin Song, Yu Wang, Yongzhi Cao, Mengji Hu, Tao Zhou, Xueping Viruses Article A novel RNA virus was identified in firespike (Odontonema tubaeforme) plants exhibiting leaf curling and chlorosis. The molecular features of the viral genomic RNA and proteins resemble those of ampeloviruses. Based on sequence comparisons and phylogenetic analysis, we propose a new species in the genus Ampelovirus, which we have tentatively named Firespike leafroll-associated virus (FLRaV). Bioassays showed that the virus is mechanically transmissible to Nicotiana benthamiana. In addition, a full-length cDNA clone of FLRaV could successfully infect N. benthamiana via agroinfiltration. MDPI 2020-12-16 /pmc/articles/PMC7766885/ /pubmed/33339381 http://dx.doi.org/10.3390/v12121452 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Yaqin
Song, Yu
Wang, Yongzhi
Cao, Mengji
Hu, Tao
Zhou, Xueping
Discovery and Characterization of a Novel Ampelovirus on Firespike
title Discovery and Characterization of a Novel Ampelovirus on Firespike
title_full Discovery and Characterization of a Novel Ampelovirus on Firespike
title_fullStr Discovery and Characterization of a Novel Ampelovirus on Firespike
title_full_unstemmed Discovery and Characterization of a Novel Ampelovirus on Firespike
title_short Discovery and Characterization of a Novel Ampelovirus on Firespike
title_sort discovery and characterization of a novel ampelovirus on firespike
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7766885/
https://www.ncbi.nlm.nih.gov/pubmed/33339381
http://dx.doi.org/10.3390/v12121452
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