Highly Sensitive Detection of CA 125 Protein with the Use of an n-Type Nanowire Biosensor

The detection of CA 125 protein in a solution using a silicon-on-insulator (SOI)-nanowire biosensor with n-type chip has been experimentally demonstrated. The surface of nanowires was modified by covalent immobilization of antibodies against CA 125 in order to provide the biospecificity of the targe...

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Detalles Bibliográficos
Autores principales: Malsagova, Kristina A., Pleshakova, Tatyana O., Galiullin, Rafael A., Kozlov, Andrey F., Shumov, Ivan D., Popov, Vladimir P., Tikhonenko, Fedor V., Glukhov, Alexander V., Ziborov, Vadim S., Petrov, Oleg F., Fortov, Vladimir E., Archakov, Alexander I., Ivanov, Yuri D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7766891/
https://www.ncbi.nlm.nih.gov/pubmed/33353197
http://dx.doi.org/10.3390/bios10120210
Descripción
Sumario:The detection of CA 125 protein in a solution using a silicon-on-insulator (SOI)-nanowire biosensor with n-type chip has been experimentally demonstrated. The surface of nanowires was modified by covalent immobilization of antibodies against CA 125 in order to provide the biospecificity of the target protein detection. We have demonstrated that the biosensor signal, which results from the biospecific interaction between CA 125 and the covalently immobilized antibodies, increases with the increase in the protein concentration. At that, the minimum concentration, at which the target protein was detectable with the SOI-nanowire biosensor, amounted to 1.5 × 10(−16) M.

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