Cargando…

Clinical Biofilm Ring Test(®) Reveals the Potential Role of β-Lactams in the Induction of Biofilm Formation by P. aeruginosa in Cystic Fibrosis Patients

Biofilms are characterized by high tolerance to antimicrobials. However, conventional antibiograms are performed on planktonic microorganisms. Through the clinical Biofilm Ring Test(®) (cBRT), initially aimed to measure the adhesion propensity of bacteria, we discerned a variable distribution of bio...

Descripción completa

Detalles Bibliográficos
Autores principales: Olivares, Elodie, Tasse, Jason, Badel-Berchoux, Stéphanie, Provot, Christian, Prévost, Gilles, Bernardi, Thierry
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7766936/
https://www.ncbi.nlm.nih.gov/pubmed/33352641
http://dx.doi.org/10.3390/pathogens9121065
_version_ 1783628837898158080
author Olivares, Elodie
Tasse, Jason
Badel-Berchoux, Stéphanie
Provot, Christian
Prévost, Gilles
Bernardi, Thierry
author_facet Olivares, Elodie
Tasse, Jason
Badel-Berchoux, Stéphanie
Provot, Christian
Prévost, Gilles
Bernardi, Thierry
author_sort Olivares, Elodie
collection PubMed
description Biofilms are characterized by high tolerance to antimicrobials. However, conventional antibiograms are performed on planktonic microorganisms. Through the clinical Biofilm Ring Test(®) (cBRT), initially aimed to measure the adhesion propensity of bacteria, we discerned a variable distribution of biofilm-producer strains among P. aeruginosa samples isolated from expectorations of cystic fibrosis (CF) patients. Despite a majority of spontaneous adherent isolates, few strains remained planktonic after 5 h of incubation. Their analysis by an adapted protocol of the cBRT revealed an induction of the biofilm early formation by sub-inhibitory doses of β-lactams. Microscopic observations of bacterial cultures stained with Syto 9/Propidium Iodide (PI) confirmed the ability of antimicrobials to increase either the bacterial biomass or the biovolume occupied by induced sessile cells. Finally, the cBRT and its derivatives enabled to highlight in a few hours the potential inducer property of antibiotics on bacterial adhesion. This phenomenon should be considered carefully in the context of CF since patients are constantly under fluctuating antimicrobial treatments. To conclude, assays derived from the Biofilm Ring Test(®) (BRT) device, not only define efficient doses preventing biofilm formation, but could be useful for the antimicrobial selection in CF, to avoid inducer molecules of the early biofilm initiation.
format Online
Article
Text
id pubmed-7766936
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-77669362020-12-28 Clinical Biofilm Ring Test(®) Reveals the Potential Role of β-Lactams in the Induction of Biofilm Formation by P. aeruginosa in Cystic Fibrosis Patients Olivares, Elodie Tasse, Jason Badel-Berchoux, Stéphanie Provot, Christian Prévost, Gilles Bernardi, Thierry Pathogens Article Biofilms are characterized by high tolerance to antimicrobials. However, conventional antibiograms are performed on planktonic microorganisms. Through the clinical Biofilm Ring Test(®) (cBRT), initially aimed to measure the adhesion propensity of bacteria, we discerned a variable distribution of biofilm-producer strains among P. aeruginosa samples isolated from expectorations of cystic fibrosis (CF) patients. Despite a majority of spontaneous adherent isolates, few strains remained planktonic after 5 h of incubation. Their analysis by an adapted protocol of the cBRT revealed an induction of the biofilm early formation by sub-inhibitory doses of β-lactams. Microscopic observations of bacterial cultures stained with Syto 9/Propidium Iodide (PI) confirmed the ability of antimicrobials to increase either the bacterial biomass or the biovolume occupied by induced sessile cells. Finally, the cBRT and its derivatives enabled to highlight in a few hours the potential inducer property of antibiotics on bacterial adhesion. This phenomenon should be considered carefully in the context of CF since patients are constantly under fluctuating antimicrobial treatments. To conclude, assays derived from the Biofilm Ring Test(®) (BRT) device, not only define efficient doses preventing biofilm formation, but could be useful for the antimicrobial selection in CF, to avoid inducer molecules of the early biofilm initiation. MDPI 2020-12-19 /pmc/articles/PMC7766936/ /pubmed/33352641 http://dx.doi.org/10.3390/pathogens9121065 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Olivares, Elodie
Tasse, Jason
Badel-Berchoux, Stéphanie
Provot, Christian
Prévost, Gilles
Bernardi, Thierry
Clinical Biofilm Ring Test(®) Reveals the Potential Role of β-Lactams in the Induction of Biofilm Formation by P. aeruginosa in Cystic Fibrosis Patients
title Clinical Biofilm Ring Test(®) Reveals the Potential Role of β-Lactams in the Induction of Biofilm Formation by P. aeruginosa in Cystic Fibrosis Patients
title_full Clinical Biofilm Ring Test(®) Reveals the Potential Role of β-Lactams in the Induction of Biofilm Formation by P. aeruginosa in Cystic Fibrosis Patients
title_fullStr Clinical Biofilm Ring Test(®) Reveals the Potential Role of β-Lactams in the Induction of Biofilm Formation by P. aeruginosa in Cystic Fibrosis Patients
title_full_unstemmed Clinical Biofilm Ring Test(®) Reveals the Potential Role of β-Lactams in the Induction of Biofilm Formation by P. aeruginosa in Cystic Fibrosis Patients
title_short Clinical Biofilm Ring Test(®) Reveals the Potential Role of β-Lactams in the Induction of Biofilm Formation by P. aeruginosa in Cystic Fibrosis Patients
title_sort clinical biofilm ring test(®) reveals the potential role of β-lactams in the induction of biofilm formation by p. aeruginosa in cystic fibrosis patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7766936/
https://www.ncbi.nlm.nih.gov/pubmed/33352641
http://dx.doi.org/10.3390/pathogens9121065
work_keys_str_mv AT olivareselodie clinicalbiofilmringtestrevealsthepotentialroleofblactamsintheinductionofbiofilmformationbypaeruginosaincysticfibrosispatients
AT tassejason clinicalbiofilmringtestrevealsthepotentialroleofblactamsintheinductionofbiofilmformationbypaeruginosaincysticfibrosispatients
AT badelberchouxstephanie clinicalbiofilmringtestrevealsthepotentialroleofblactamsintheinductionofbiofilmformationbypaeruginosaincysticfibrosispatients
AT provotchristian clinicalbiofilmringtestrevealsthepotentialroleofblactamsintheinductionofbiofilmformationbypaeruginosaincysticfibrosispatients
AT prevostgilles clinicalbiofilmringtestrevealsthepotentialroleofblactamsintheinductionofbiofilmformationbypaeruginosaincysticfibrosispatients
AT bernardithierry clinicalbiofilmringtestrevealsthepotentialroleofblactamsintheinductionofbiofilmformationbypaeruginosaincysticfibrosispatients