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Clinical Biofilm Ring Test(®) Reveals the Potential Role of β-Lactams in the Induction of Biofilm Formation by P. aeruginosa in Cystic Fibrosis Patients
Biofilms are characterized by high tolerance to antimicrobials. However, conventional antibiograms are performed on planktonic microorganisms. Through the clinical Biofilm Ring Test(®) (cBRT), initially aimed to measure the adhesion propensity of bacteria, we discerned a variable distribution of bio...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7766936/ https://www.ncbi.nlm.nih.gov/pubmed/33352641 http://dx.doi.org/10.3390/pathogens9121065 |
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author | Olivares, Elodie Tasse, Jason Badel-Berchoux, Stéphanie Provot, Christian Prévost, Gilles Bernardi, Thierry |
author_facet | Olivares, Elodie Tasse, Jason Badel-Berchoux, Stéphanie Provot, Christian Prévost, Gilles Bernardi, Thierry |
author_sort | Olivares, Elodie |
collection | PubMed |
description | Biofilms are characterized by high tolerance to antimicrobials. However, conventional antibiograms are performed on planktonic microorganisms. Through the clinical Biofilm Ring Test(®) (cBRT), initially aimed to measure the adhesion propensity of bacteria, we discerned a variable distribution of biofilm-producer strains among P. aeruginosa samples isolated from expectorations of cystic fibrosis (CF) patients. Despite a majority of spontaneous adherent isolates, few strains remained planktonic after 5 h of incubation. Their analysis by an adapted protocol of the cBRT revealed an induction of the biofilm early formation by sub-inhibitory doses of β-lactams. Microscopic observations of bacterial cultures stained with Syto 9/Propidium Iodide (PI) confirmed the ability of antimicrobials to increase either the bacterial biomass or the biovolume occupied by induced sessile cells. Finally, the cBRT and its derivatives enabled to highlight in a few hours the potential inducer property of antibiotics on bacterial adhesion. This phenomenon should be considered carefully in the context of CF since patients are constantly under fluctuating antimicrobial treatments. To conclude, assays derived from the Biofilm Ring Test(®) (BRT) device, not only define efficient doses preventing biofilm formation, but could be useful for the antimicrobial selection in CF, to avoid inducer molecules of the early biofilm initiation. |
format | Online Article Text |
id | pubmed-7766936 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77669362020-12-28 Clinical Biofilm Ring Test(®) Reveals the Potential Role of β-Lactams in the Induction of Biofilm Formation by P. aeruginosa in Cystic Fibrosis Patients Olivares, Elodie Tasse, Jason Badel-Berchoux, Stéphanie Provot, Christian Prévost, Gilles Bernardi, Thierry Pathogens Article Biofilms are characterized by high tolerance to antimicrobials. However, conventional antibiograms are performed on planktonic microorganisms. Through the clinical Biofilm Ring Test(®) (cBRT), initially aimed to measure the adhesion propensity of bacteria, we discerned a variable distribution of biofilm-producer strains among P. aeruginosa samples isolated from expectorations of cystic fibrosis (CF) patients. Despite a majority of spontaneous adherent isolates, few strains remained planktonic after 5 h of incubation. Their analysis by an adapted protocol of the cBRT revealed an induction of the biofilm early formation by sub-inhibitory doses of β-lactams. Microscopic observations of bacterial cultures stained with Syto 9/Propidium Iodide (PI) confirmed the ability of antimicrobials to increase either the bacterial biomass or the biovolume occupied by induced sessile cells. Finally, the cBRT and its derivatives enabled to highlight in a few hours the potential inducer property of antibiotics on bacterial adhesion. This phenomenon should be considered carefully in the context of CF since patients are constantly under fluctuating antimicrobial treatments. To conclude, assays derived from the Biofilm Ring Test(®) (BRT) device, not only define efficient doses preventing biofilm formation, but could be useful for the antimicrobial selection in CF, to avoid inducer molecules of the early biofilm initiation. MDPI 2020-12-19 /pmc/articles/PMC7766936/ /pubmed/33352641 http://dx.doi.org/10.3390/pathogens9121065 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Olivares, Elodie Tasse, Jason Badel-Berchoux, Stéphanie Provot, Christian Prévost, Gilles Bernardi, Thierry Clinical Biofilm Ring Test(®) Reveals the Potential Role of β-Lactams in the Induction of Biofilm Formation by P. aeruginosa in Cystic Fibrosis Patients |
title | Clinical Biofilm Ring Test(®) Reveals the Potential Role of β-Lactams in the Induction of Biofilm Formation by P. aeruginosa in Cystic Fibrosis Patients |
title_full | Clinical Biofilm Ring Test(®) Reveals the Potential Role of β-Lactams in the Induction of Biofilm Formation by P. aeruginosa in Cystic Fibrosis Patients |
title_fullStr | Clinical Biofilm Ring Test(®) Reveals the Potential Role of β-Lactams in the Induction of Biofilm Formation by P. aeruginosa in Cystic Fibrosis Patients |
title_full_unstemmed | Clinical Biofilm Ring Test(®) Reveals the Potential Role of β-Lactams in the Induction of Biofilm Formation by P. aeruginosa in Cystic Fibrosis Patients |
title_short | Clinical Biofilm Ring Test(®) Reveals the Potential Role of β-Lactams in the Induction of Biofilm Formation by P. aeruginosa in Cystic Fibrosis Patients |
title_sort | clinical biofilm ring test(®) reveals the potential role of β-lactams in the induction of biofilm formation by p. aeruginosa in cystic fibrosis patients |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7766936/ https://www.ncbi.nlm.nih.gov/pubmed/33352641 http://dx.doi.org/10.3390/pathogens9121065 |
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