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Extraction Improvement of the Bioactive Blue-Green Pigment “Marennine” from Diatom Haslea ostrearia’s Blue Water: A Solid-Phase Method Based on Graphitic Matrices

The compound “marennine” is a blue–green pigment produced by the benthic microalgae Haslea ostrearia, with pathogenicity reduction activities against some bacteria and promising potential as a natural pigment in seafood industries. After decades of research, the chemical family of this compound stil...

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Autores principales: Bélanger, William, Arnold, Alexandre A., Turcotte, François, Saint-Louis, Richard, Deschênes, Jean-Sébastien, Genard, Bertrand, Marcotte, Isabelle, Tremblay, Réjean
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7767052/
https://www.ncbi.nlm.nih.gov/pubmed/33352967
http://dx.doi.org/10.3390/md18120653
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author Bélanger, William
Arnold, Alexandre A.
Turcotte, François
Saint-Louis, Richard
Deschênes, Jean-Sébastien
Genard, Bertrand
Marcotte, Isabelle
Tremblay, Réjean
author_facet Bélanger, William
Arnold, Alexandre A.
Turcotte, François
Saint-Louis, Richard
Deschênes, Jean-Sébastien
Genard, Bertrand
Marcotte, Isabelle
Tremblay, Réjean
author_sort Bélanger, William
collection PubMed
description The compound “marennine” is a blue–green pigment produced by the benthic microalgae Haslea ostrearia, with pathogenicity reduction activities against some bacteria and promising potential as a natural pigment in seafood industries. After decades of research, the chemical family of this compound still remains unclear, mainly because structural studies were impaired by the presence of co-extracted compounds in marennine isolates. To improve the purity of marennine extract, we developed a novel extraction method using a graphitic stationary phase, which provides various advantages over the previous procedure using tandem ultrafiltration. Our method is faster, more versatile, provides a better crude yield (66%, compared to 57% for ultrafiltration) and is amenable to upscaling with continuous photobioreactor cultivation. Our goal was to take advantage of the modulable surface properties of the graphitic matrix by optimizing its interactions with marennine. As such, the effects of organic modifiers, pH and reducing agents were studied. With this improvement on marennine purification, we achieved altogether the isolation of a fucoidan-related, sulfated polysaccharide from blue water. Characterization of the polysaccharides fraction suggests that roughly half of UV-absorbing compounds could be isolated from the marennine crude extracts. The identification of sulfated polysaccharides could be a major breakthrough for marennine purification, providing targeted isolation techniques. Likewise, the added value of Haslea ostrearia and the role of polysaccharides in previous marennine chemical characterization and bioactivity studies remain to be determined.
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spelling pubmed-77670522020-12-28 Extraction Improvement of the Bioactive Blue-Green Pigment “Marennine” from Diatom Haslea ostrearia’s Blue Water: A Solid-Phase Method Based on Graphitic Matrices Bélanger, William Arnold, Alexandre A. Turcotte, François Saint-Louis, Richard Deschênes, Jean-Sébastien Genard, Bertrand Marcotte, Isabelle Tremblay, Réjean Mar Drugs Article The compound “marennine” is a blue–green pigment produced by the benthic microalgae Haslea ostrearia, with pathogenicity reduction activities against some bacteria and promising potential as a natural pigment in seafood industries. After decades of research, the chemical family of this compound still remains unclear, mainly because structural studies were impaired by the presence of co-extracted compounds in marennine isolates. To improve the purity of marennine extract, we developed a novel extraction method using a graphitic stationary phase, which provides various advantages over the previous procedure using tandem ultrafiltration. Our method is faster, more versatile, provides a better crude yield (66%, compared to 57% for ultrafiltration) and is amenable to upscaling with continuous photobioreactor cultivation. Our goal was to take advantage of the modulable surface properties of the graphitic matrix by optimizing its interactions with marennine. As such, the effects of organic modifiers, pH and reducing agents were studied. With this improvement on marennine purification, we achieved altogether the isolation of a fucoidan-related, sulfated polysaccharide from blue water. Characterization of the polysaccharides fraction suggests that roughly half of UV-absorbing compounds could be isolated from the marennine crude extracts. The identification of sulfated polysaccharides could be a major breakthrough for marennine purification, providing targeted isolation techniques. Likewise, the added value of Haslea ostrearia and the role of polysaccharides in previous marennine chemical characterization and bioactivity studies remain to be determined. MDPI 2020-12-18 /pmc/articles/PMC7767052/ /pubmed/33352967 http://dx.doi.org/10.3390/md18120653 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bélanger, William
Arnold, Alexandre A.
Turcotte, François
Saint-Louis, Richard
Deschênes, Jean-Sébastien
Genard, Bertrand
Marcotte, Isabelle
Tremblay, Réjean
Extraction Improvement of the Bioactive Blue-Green Pigment “Marennine” from Diatom Haslea ostrearia’s Blue Water: A Solid-Phase Method Based on Graphitic Matrices
title Extraction Improvement of the Bioactive Blue-Green Pigment “Marennine” from Diatom Haslea ostrearia’s Blue Water: A Solid-Phase Method Based on Graphitic Matrices
title_full Extraction Improvement of the Bioactive Blue-Green Pigment “Marennine” from Diatom Haslea ostrearia’s Blue Water: A Solid-Phase Method Based on Graphitic Matrices
title_fullStr Extraction Improvement of the Bioactive Blue-Green Pigment “Marennine” from Diatom Haslea ostrearia’s Blue Water: A Solid-Phase Method Based on Graphitic Matrices
title_full_unstemmed Extraction Improvement of the Bioactive Blue-Green Pigment “Marennine” from Diatom Haslea ostrearia’s Blue Water: A Solid-Phase Method Based on Graphitic Matrices
title_short Extraction Improvement of the Bioactive Blue-Green Pigment “Marennine” from Diatom Haslea ostrearia’s Blue Water: A Solid-Phase Method Based on Graphitic Matrices
title_sort extraction improvement of the bioactive blue-green pigment “marennine” from diatom haslea ostrearia’s blue water: a solid-phase method based on graphitic matrices
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7767052/
https://www.ncbi.nlm.nih.gov/pubmed/33352967
http://dx.doi.org/10.3390/md18120653
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