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Extraction Improvement of the Bioactive Blue-Green Pigment “Marennine” from Diatom Haslea ostrearia’s Blue Water: A Solid-Phase Method Based on Graphitic Matrices
The compound “marennine” is a blue–green pigment produced by the benthic microalgae Haslea ostrearia, with pathogenicity reduction activities against some bacteria and promising potential as a natural pigment in seafood industries. After decades of research, the chemical family of this compound stil...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7767052/ https://www.ncbi.nlm.nih.gov/pubmed/33352967 http://dx.doi.org/10.3390/md18120653 |
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author | Bélanger, William Arnold, Alexandre A. Turcotte, François Saint-Louis, Richard Deschênes, Jean-Sébastien Genard, Bertrand Marcotte, Isabelle Tremblay, Réjean |
author_facet | Bélanger, William Arnold, Alexandre A. Turcotte, François Saint-Louis, Richard Deschênes, Jean-Sébastien Genard, Bertrand Marcotte, Isabelle Tremblay, Réjean |
author_sort | Bélanger, William |
collection | PubMed |
description | The compound “marennine” is a blue–green pigment produced by the benthic microalgae Haslea ostrearia, with pathogenicity reduction activities against some bacteria and promising potential as a natural pigment in seafood industries. After decades of research, the chemical family of this compound still remains unclear, mainly because structural studies were impaired by the presence of co-extracted compounds in marennine isolates. To improve the purity of marennine extract, we developed a novel extraction method using a graphitic stationary phase, which provides various advantages over the previous procedure using tandem ultrafiltration. Our method is faster, more versatile, provides a better crude yield (66%, compared to 57% for ultrafiltration) and is amenable to upscaling with continuous photobioreactor cultivation. Our goal was to take advantage of the modulable surface properties of the graphitic matrix by optimizing its interactions with marennine. As such, the effects of organic modifiers, pH and reducing agents were studied. With this improvement on marennine purification, we achieved altogether the isolation of a fucoidan-related, sulfated polysaccharide from blue water. Characterization of the polysaccharides fraction suggests that roughly half of UV-absorbing compounds could be isolated from the marennine crude extracts. The identification of sulfated polysaccharides could be a major breakthrough for marennine purification, providing targeted isolation techniques. Likewise, the added value of Haslea ostrearia and the role of polysaccharides in previous marennine chemical characterization and bioactivity studies remain to be determined. |
format | Online Article Text |
id | pubmed-7767052 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77670522020-12-28 Extraction Improvement of the Bioactive Blue-Green Pigment “Marennine” from Diatom Haslea ostrearia’s Blue Water: A Solid-Phase Method Based on Graphitic Matrices Bélanger, William Arnold, Alexandre A. Turcotte, François Saint-Louis, Richard Deschênes, Jean-Sébastien Genard, Bertrand Marcotte, Isabelle Tremblay, Réjean Mar Drugs Article The compound “marennine” is a blue–green pigment produced by the benthic microalgae Haslea ostrearia, with pathogenicity reduction activities against some bacteria and promising potential as a natural pigment in seafood industries. After decades of research, the chemical family of this compound still remains unclear, mainly because structural studies were impaired by the presence of co-extracted compounds in marennine isolates. To improve the purity of marennine extract, we developed a novel extraction method using a graphitic stationary phase, which provides various advantages over the previous procedure using tandem ultrafiltration. Our method is faster, more versatile, provides a better crude yield (66%, compared to 57% for ultrafiltration) and is amenable to upscaling with continuous photobioreactor cultivation. Our goal was to take advantage of the modulable surface properties of the graphitic matrix by optimizing its interactions with marennine. As such, the effects of organic modifiers, pH and reducing agents were studied. With this improvement on marennine purification, we achieved altogether the isolation of a fucoidan-related, sulfated polysaccharide from blue water. Characterization of the polysaccharides fraction suggests that roughly half of UV-absorbing compounds could be isolated from the marennine crude extracts. The identification of sulfated polysaccharides could be a major breakthrough for marennine purification, providing targeted isolation techniques. Likewise, the added value of Haslea ostrearia and the role of polysaccharides in previous marennine chemical characterization and bioactivity studies remain to be determined. MDPI 2020-12-18 /pmc/articles/PMC7767052/ /pubmed/33352967 http://dx.doi.org/10.3390/md18120653 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bélanger, William Arnold, Alexandre A. Turcotte, François Saint-Louis, Richard Deschênes, Jean-Sébastien Genard, Bertrand Marcotte, Isabelle Tremblay, Réjean Extraction Improvement of the Bioactive Blue-Green Pigment “Marennine” from Diatom Haslea ostrearia’s Blue Water: A Solid-Phase Method Based on Graphitic Matrices |
title | Extraction Improvement of the Bioactive Blue-Green Pigment “Marennine” from Diatom Haslea ostrearia’s Blue Water: A Solid-Phase Method Based on Graphitic Matrices |
title_full | Extraction Improvement of the Bioactive Blue-Green Pigment “Marennine” from Diatom Haslea ostrearia’s Blue Water: A Solid-Phase Method Based on Graphitic Matrices |
title_fullStr | Extraction Improvement of the Bioactive Blue-Green Pigment “Marennine” from Diatom Haslea ostrearia’s Blue Water: A Solid-Phase Method Based on Graphitic Matrices |
title_full_unstemmed | Extraction Improvement of the Bioactive Blue-Green Pigment “Marennine” from Diatom Haslea ostrearia’s Blue Water: A Solid-Phase Method Based on Graphitic Matrices |
title_short | Extraction Improvement of the Bioactive Blue-Green Pigment “Marennine” from Diatom Haslea ostrearia’s Blue Water: A Solid-Phase Method Based on Graphitic Matrices |
title_sort | extraction improvement of the bioactive blue-green pigment “marennine” from diatom haslea ostrearia’s blue water: a solid-phase method based on graphitic matrices |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7767052/ https://www.ncbi.nlm.nih.gov/pubmed/33352967 http://dx.doi.org/10.3390/md18120653 |
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