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Response of NIH 3T3 Fibroblast Cells on Laser-Induced Periodic Surface Structures on a 15×(Ti/Zr)/Si Multilayer System
Ultrafast laser processing with the formation of periodic surface nanostructures on the 15×(Ti/Zr)/Si multilayers is studied in order to the improve cell response. A novel nanocomposite structure in the form of 15×(Ti/Zr)/Si multilayer thin films, with satisfying mechanical properties and moderate b...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7767124/ https://www.ncbi.nlm.nih.gov/pubmed/33339399 http://dx.doi.org/10.3390/nano10122531 |
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author | Petrović, Suzana Peruško, Davor Mimidis, Alexandros Kavatzikidou, Paraskeva Kovač, Janez Ranella, Anthi Novaković, Mirjana Popović, Maja Stratakis, Emmanuel |
author_facet | Petrović, Suzana Peruško, Davor Mimidis, Alexandros Kavatzikidou, Paraskeva Kovač, Janez Ranella, Anthi Novaković, Mirjana Popović, Maja Stratakis, Emmanuel |
author_sort | Petrović, Suzana |
collection | PubMed |
description | Ultrafast laser processing with the formation of periodic surface nanostructures on the 15×(Ti/Zr)/Si multilayers is studied in order to the improve cell response. A novel nanocomposite structure in the form of 15×(Ti/Zr)/Si multilayer thin films, with satisfying mechanical properties and moderate biocompatibility, was deposited by ion sputtering on an Si substrate. The multilayer 15×(Ti/Zr)/Si thin films were modified by femtosecond laser pulses in air to induce the following modifications: (i) mixing of components inside of the multilayer structures, (ii) the formation of an ultrathin oxide layer at the surfaces, and (iii) surface nano-texturing with the creation of laser-induced periodic surface structure (LIPSS). The focus of this study was an examination of the novel Ti/Zr multilayer thin films in order to create a surface texture with suitable composition and structure for cell integration. Using the SEM and confocal microscopies of the laser-modified Ti/Zr surfaces with seeded cell culture (NIH 3T3 fibroblasts), it was found that cell adhesion and growth depend on the surface composition and morphological patterns. These results indicated a good proliferation of cells after two and four days with some tendency of the cell orientation along the LIPSSs. |
format | Online Article Text |
id | pubmed-7767124 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77671242020-12-28 Response of NIH 3T3 Fibroblast Cells on Laser-Induced Periodic Surface Structures on a 15×(Ti/Zr)/Si Multilayer System Petrović, Suzana Peruško, Davor Mimidis, Alexandros Kavatzikidou, Paraskeva Kovač, Janez Ranella, Anthi Novaković, Mirjana Popović, Maja Stratakis, Emmanuel Nanomaterials (Basel) Article Ultrafast laser processing with the formation of periodic surface nanostructures on the 15×(Ti/Zr)/Si multilayers is studied in order to the improve cell response. A novel nanocomposite structure in the form of 15×(Ti/Zr)/Si multilayer thin films, with satisfying mechanical properties and moderate biocompatibility, was deposited by ion sputtering on an Si substrate. The multilayer 15×(Ti/Zr)/Si thin films were modified by femtosecond laser pulses in air to induce the following modifications: (i) mixing of components inside of the multilayer structures, (ii) the formation of an ultrathin oxide layer at the surfaces, and (iii) surface nano-texturing with the creation of laser-induced periodic surface structure (LIPSS). The focus of this study was an examination of the novel Ti/Zr multilayer thin films in order to create a surface texture with suitable composition and structure for cell integration. Using the SEM and confocal microscopies of the laser-modified Ti/Zr surfaces with seeded cell culture (NIH 3T3 fibroblasts), it was found that cell adhesion and growth depend on the surface composition and morphological patterns. These results indicated a good proliferation of cells after two and four days with some tendency of the cell orientation along the LIPSSs. MDPI 2020-12-16 /pmc/articles/PMC7767124/ /pubmed/33339399 http://dx.doi.org/10.3390/nano10122531 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Petrović, Suzana Peruško, Davor Mimidis, Alexandros Kavatzikidou, Paraskeva Kovač, Janez Ranella, Anthi Novaković, Mirjana Popović, Maja Stratakis, Emmanuel Response of NIH 3T3 Fibroblast Cells on Laser-Induced Periodic Surface Structures on a 15×(Ti/Zr)/Si Multilayer System |
title | Response of NIH 3T3 Fibroblast Cells on Laser-Induced Periodic Surface Structures on a 15×(Ti/Zr)/Si Multilayer System |
title_full | Response of NIH 3T3 Fibroblast Cells on Laser-Induced Periodic Surface Structures on a 15×(Ti/Zr)/Si Multilayer System |
title_fullStr | Response of NIH 3T3 Fibroblast Cells on Laser-Induced Periodic Surface Structures on a 15×(Ti/Zr)/Si Multilayer System |
title_full_unstemmed | Response of NIH 3T3 Fibroblast Cells on Laser-Induced Periodic Surface Structures on a 15×(Ti/Zr)/Si Multilayer System |
title_short | Response of NIH 3T3 Fibroblast Cells on Laser-Induced Periodic Surface Structures on a 15×(Ti/Zr)/Si Multilayer System |
title_sort | response of nih 3t3 fibroblast cells on laser-induced periodic surface structures on a 15×(ti/zr)/si multilayer system |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7767124/ https://www.ncbi.nlm.nih.gov/pubmed/33339399 http://dx.doi.org/10.3390/nano10122531 |
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