Cargando…

Response of NIH 3T3 Fibroblast Cells on Laser-Induced Periodic Surface Structures on a 15×(Ti/Zr)/Si Multilayer System

Ultrafast laser processing with the formation of periodic surface nanostructures on the 15×(Ti/Zr)/Si multilayers is studied in order to the improve cell response. A novel nanocomposite structure in the form of 15×(Ti/Zr)/Si multilayer thin films, with satisfying mechanical properties and moderate b...

Descripción completa

Detalles Bibliográficos
Autores principales: Petrović, Suzana, Peruško, Davor, Mimidis, Alexandros, Kavatzikidou, Paraskeva, Kovač, Janez, Ranella, Anthi, Novaković, Mirjana, Popović, Maja, Stratakis, Emmanuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7767124/
https://www.ncbi.nlm.nih.gov/pubmed/33339399
http://dx.doi.org/10.3390/nano10122531
_version_ 1783628882199445504
author Petrović, Suzana
Peruško, Davor
Mimidis, Alexandros
Kavatzikidou, Paraskeva
Kovač, Janez
Ranella, Anthi
Novaković, Mirjana
Popović, Maja
Stratakis, Emmanuel
author_facet Petrović, Suzana
Peruško, Davor
Mimidis, Alexandros
Kavatzikidou, Paraskeva
Kovač, Janez
Ranella, Anthi
Novaković, Mirjana
Popović, Maja
Stratakis, Emmanuel
author_sort Petrović, Suzana
collection PubMed
description Ultrafast laser processing with the formation of periodic surface nanostructures on the 15×(Ti/Zr)/Si multilayers is studied in order to the improve cell response. A novel nanocomposite structure in the form of 15×(Ti/Zr)/Si multilayer thin films, with satisfying mechanical properties and moderate biocompatibility, was deposited by ion sputtering on an Si substrate. The multilayer 15×(Ti/Zr)/Si thin films were modified by femtosecond laser pulses in air to induce the following modifications: (i) mixing of components inside of the multilayer structures, (ii) the formation of an ultrathin oxide layer at the surfaces, and (iii) surface nano-texturing with the creation of laser-induced periodic surface structure (LIPSS). The focus of this study was an examination of the novel Ti/Zr multilayer thin films in order to create a surface texture with suitable composition and structure for cell integration. Using the SEM and confocal microscopies of the laser-modified Ti/Zr surfaces with seeded cell culture (NIH 3T3 fibroblasts), it was found that cell adhesion and growth depend on the surface composition and morphological patterns. These results indicated a good proliferation of cells after two and four days with some tendency of the cell orientation along the LIPSSs.
format Online
Article
Text
id pubmed-7767124
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-77671242020-12-28 Response of NIH 3T3 Fibroblast Cells on Laser-Induced Periodic Surface Structures on a 15×(Ti/Zr)/Si Multilayer System Petrović, Suzana Peruško, Davor Mimidis, Alexandros Kavatzikidou, Paraskeva Kovač, Janez Ranella, Anthi Novaković, Mirjana Popović, Maja Stratakis, Emmanuel Nanomaterials (Basel) Article Ultrafast laser processing with the formation of periodic surface nanostructures on the 15×(Ti/Zr)/Si multilayers is studied in order to the improve cell response. A novel nanocomposite structure in the form of 15×(Ti/Zr)/Si multilayer thin films, with satisfying mechanical properties and moderate biocompatibility, was deposited by ion sputtering on an Si substrate. The multilayer 15×(Ti/Zr)/Si thin films were modified by femtosecond laser pulses in air to induce the following modifications: (i) mixing of components inside of the multilayer structures, (ii) the formation of an ultrathin oxide layer at the surfaces, and (iii) surface nano-texturing with the creation of laser-induced periodic surface structure (LIPSS). The focus of this study was an examination of the novel Ti/Zr multilayer thin films in order to create a surface texture with suitable composition and structure for cell integration. Using the SEM and confocal microscopies of the laser-modified Ti/Zr surfaces with seeded cell culture (NIH 3T3 fibroblasts), it was found that cell adhesion and growth depend on the surface composition and morphological patterns. These results indicated a good proliferation of cells after two and four days with some tendency of the cell orientation along the LIPSSs. MDPI 2020-12-16 /pmc/articles/PMC7767124/ /pubmed/33339399 http://dx.doi.org/10.3390/nano10122531 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Petrović, Suzana
Peruško, Davor
Mimidis, Alexandros
Kavatzikidou, Paraskeva
Kovač, Janez
Ranella, Anthi
Novaković, Mirjana
Popović, Maja
Stratakis, Emmanuel
Response of NIH 3T3 Fibroblast Cells on Laser-Induced Periodic Surface Structures on a 15×(Ti/Zr)/Si Multilayer System
title Response of NIH 3T3 Fibroblast Cells on Laser-Induced Periodic Surface Structures on a 15×(Ti/Zr)/Si Multilayer System
title_full Response of NIH 3T3 Fibroblast Cells on Laser-Induced Periodic Surface Structures on a 15×(Ti/Zr)/Si Multilayer System
title_fullStr Response of NIH 3T3 Fibroblast Cells on Laser-Induced Periodic Surface Structures on a 15×(Ti/Zr)/Si Multilayer System
title_full_unstemmed Response of NIH 3T3 Fibroblast Cells on Laser-Induced Periodic Surface Structures on a 15×(Ti/Zr)/Si Multilayer System
title_short Response of NIH 3T3 Fibroblast Cells on Laser-Induced Periodic Surface Structures on a 15×(Ti/Zr)/Si Multilayer System
title_sort response of nih 3t3 fibroblast cells on laser-induced periodic surface structures on a 15×(ti/zr)/si multilayer system
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7767124/
https://www.ncbi.nlm.nih.gov/pubmed/33339399
http://dx.doi.org/10.3390/nano10122531
work_keys_str_mv AT petrovicsuzana responseofnih3t3fibroblastcellsonlaserinducedperiodicsurfacestructuresona15tizrsimultilayersystem
AT peruskodavor responseofnih3t3fibroblastcellsonlaserinducedperiodicsurfacestructuresona15tizrsimultilayersystem
AT mimidisalexandros responseofnih3t3fibroblastcellsonlaserinducedperiodicsurfacestructuresona15tizrsimultilayersystem
AT kavatzikidouparaskeva responseofnih3t3fibroblastcellsonlaserinducedperiodicsurfacestructuresona15tizrsimultilayersystem
AT kovacjanez responseofnih3t3fibroblastcellsonlaserinducedperiodicsurfacestructuresona15tizrsimultilayersystem
AT ranellaanthi responseofnih3t3fibroblastcellsonlaserinducedperiodicsurfacestructuresona15tizrsimultilayersystem
AT novakovicmirjana responseofnih3t3fibroblastcellsonlaserinducedperiodicsurfacestructuresona15tizrsimultilayersystem
AT popovicmaja responseofnih3t3fibroblastcellsonlaserinducedperiodicsurfacestructuresona15tizrsimultilayersystem
AT stratakisemmanuel responseofnih3t3fibroblastcellsonlaserinducedperiodicsurfacestructuresona15tizrsimultilayersystem