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Adiponectin Exerts Peripheral Inhibitory Effects on the Mouse Gastric Smooth Muscle through the AMPK Pathway

Some adipokines, such as adiponectin (ADPN), other than being implicated in the central regulation of feeding behavior, may influence gastric motor responses, which are a source of peripheral signals that also influence food intake. The present study aims to elucidate the signaling pathways through...

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Autores principales: Idrizaj, Eglantina, Garella, Rachele, Nistri, Silvia, Dell’Accio, Alfonso, Cassioli, Emanuele, Rossi, Eleonora, Castellini, Giovanni, Ricca, Valdo, Squecco, Roberta, Baccari, Maria Caterina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7767160/
https://www.ncbi.nlm.nih.gov/pubmed/33348652
http://dx.doi.org/10.3390/ijms21249617
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author Idrizaj, Eglantina
Garella, Rachele
Nistri, Silvia
Dell’Accio, Alfonso
Cassioli, Emanuele
Rossi, Eleonora
Castellini, Giovanni
Ricca, Valdo
Squecco, Roberta
Baccari, Maria Caterina
author_facet Idrizaj, Eglantina
Garella, Rachele
Nistri, Silvia
Dell’Accio, Alfonso
Cassioli, Emanuele
Rossi, Eleonora
Castellini, Giovanni
Ricca, Valdo
Squecco, Roberta
Baccari, Maria Caterina
author_sort Idrizaj, Eglantina
collection PubMed
description Some adipokines, such as adiponectin (ADPN), other than being implicated in the central regulation of feeding behavior, may influence gastric motor responses, which are a source of peripheral signals that also influence food intake. The present study aims to elucidate the signaling pathways through which ADPN exerts its actions in the mouse gastric fundus. To this purpose, we used a multidisciplinary approach. The mechanical results showed that ADPN caused a decay of the strip basal tension, which was abolished by the nitric oxide (NO) synthesis inhibitor, L-N(G)-nitro arginine (L-NNA). The electrophysiological experiments confirmed that all ADPN effects were abolished by L-NNA, except for the reduction of Ca(2+) current, which was instead prevented by the inhibitor of AMP-activated protein kinase (AMPK), dorsomorphin. The activation of the AMPK signaling by ADPN was confirmed by immunofluorescence analysis, which also revealed the ADPN R1 receptor (AdipoR1) expression in glial cells of the myenteric plexus. In conclusion, our results indicate that ADPN exerts an inhibitory action on the gastric smooth muscle by acting on AdipoR1 and involving the AMPK signaling pathway at the peripheral level. These findings provide novel bases for considering AMPK as a possible pharmacologic target for the potential treatment of obesity and eating disorders.
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spelling pubmed-77671602020-12-28 Adiponectin Exerts Peripheral Inhibitory Effects on the Mouse Gastric Smooth Muscle through the AMPK Pathway Idrizaj, Eglantina Garella, Rachele Nistri, Silvia Dell’Accio, Alfonso Cassioli, Emanuele Rossi, Eleonora Castellini, Giovanni Ricca, Valdo Squecco, Roberta Baccari, Maria Caterina Int J Mol Sci Article Some adipokines, such as adiponectin (ADPN), other than being implicated in the central regulation of feeding behavior, may influence gastric motor responses, which are a source of peripheral signals that also influence food intake. The present study aims to elucidate the signaling pathways through which ADPN exerts its actions in the mouse gastric fundus. To this purpose, we used a multidisciplinary approach. The mechanical results showed that ADPN caused a decay of the strip basal tension, which was abolished by the nitric oxide (NO) synthesis inhibitor, L-N(G)-nitro arginine (L-NNA). The electrophysiological experiments confirmed that all ADPN effects were abolished by L-NNA, except for the reduction of Ca(2+) current, which was instead prevented by the inhibitor of AMP-activated protein kinase (AMPK), dorsomorphin. The activation of the AMPK signaling by ADPN was confirmed by immunofluorescence analysis, which also revealed the ADPN R1 receptor (AdipoR1) expression in glial cells of the myenteric plexus. In conclusion, our results indicate that ADPN exerts an inhibitory action on the gastric smooth muscle by acting on AdipoR1 and involving the AMPK signaling pathway at the peripheral level. These findings provide novel bases for considering AMPK as a possible pharmacologic target for the potential treatment of obesity and eating disorders. MDPI 2020-12-17 /pmc/articles/PMC7767160/ /pubmed/33348652 http://dx.doi.org/10.3390/ijms21249617 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Idrizaj, Eglantina
Garella, Rachele
Nistri, Silvia
Dell’Accio, Alfonso
Cassioli, Emanuele
Rossi, Eleonora
Castellini, Giovanni
Ricca, Valdo
Squecco, Roberta
Baccari, Maria Caterina
Adiponectin Exerts Peripheral Inhibitory Effects on the Mouse Gastric Smooth Muscle through the AMPK Pathway
title Adiponectin Exerts Peripheral Inhibitory Effects on the Mouse Gastric Smooth Muscle through the AMPK Pathway
title_full Adiponectin Exerts Peripheral Inhibitory Effects on the Mouse Gastric Smooth Muscle through the AMPK Pathway
title_fullStr Adiponectin Exerts Peripheral Inhibitory Effects on the Mouse Gastric Smooth Muscle through the AMPK Pathway
title_full_unstemmed Adiponectin Exerts Peripheral Inhibitory Effects on the Mouse Gastric Smooth Muscle through the AMPK Pathway
title_short Adiponectin Exerts Peripheral Inhibitory Effects on the Mouse Gastric Smooth Muscle through the AMPK Pathway
title_sort adiponectin exerts peripheral inhibitory effects on the mouse gastric smooth muscle through the ampk pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7767160/
https://www.ncbi.nlm.nih.gov/pubmed/33348652
http://dx.doi.org/10.3390/ijms21249617
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