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3D Reconstruction of HvRNASET2 Molecule to Understand Its Antibacterial Role
Recent studies performed on the invertebrate model Hirudo verbana (medicinal leech) suggest that the T2 ribonucleic enzyme HvRNASET2 modulates the leech’s innate immune response, promoting microbial agglutination and supporting phagocytic cells recruitment in challenged tissues. Indeed, following in...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7767213/ https://www.ncbi.nlm.nih.gov/pubmed/33352806 http://dx.doi.org/10.3390/ijms21249722 |
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author | Baranzini, Nicolò Pulze, Laura Reguzzoni, Marcella Roncoroni, Rossella Orlandi, Viviana Teresa Tettamanti, Gianluca Acquati, Francesco Grimaldi, Annalisa |
author_facet | Baranzini, Nicolò Pulze, Laura Reguzzoni, Marcella Roncoroni, Rossella Orlandi, Viviana Teresa Tettamanti, Gianluca Acquati, Francesco Grimaldi, Annalisa |
author_sort | Baranzini, Nicolò |
collection | PubMed |
description | Recent studies performed on the invertebrate model Hirudo verbana (medicinal leech) suggest that the T2 ribonucleic enzyme HvRNASET2 modulates the leech’s innate immune response, promoting microbial agglutination and supporting phagocytic cells recruitment in challenged tissues. Indeed, following injection of both lipoteichoic acid (LTA) and Staphylococcus aureus in the leech body wall, HvRNASET2 is expressed by leech type I granulocytes and induces bacterial aggregation to aid macrophage phagocytosis. Here, we investigate the HvRNASET2 antimicrobial role, in particular assessing the effects on the Gram-negative bacteria Escherichia coli. For this purpose, starting from the three-dimensional molecule reconstruction and in silico analyses, the antibacterial activity was evaluated both in vitro and in vivo. The changes induced in treated bacteria, such as agglutination and alteration in wall integrity, were observed by means of light, transmission and scanning electron microscopy. Moreover, immunogold, AMPs (antimicrobial peptides) and lipopolysaccharide (LPS) binding assays were carried out to evaluate HvRNASET2 interaction with the microbial envelopes and the ensuing ability to affect microbial viability. Finally, in vivo experiments confirmed that HvRNASET2 promotes a more rapid phagocytosis of bacterial aggregates by macrophages, representing a novel molecule for counteracting pathogen infections and developing alternative solutions to improve human health. |
format | Online Article Text |
id | pubmed-7767213 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77672132020-12-28 3D Reconstruction of HvRNASET2 Molecule to Understand Its Antibacterial Role Baranzini, Nicolò Pulze, Laura Reguzzoni, Marcella Roncoroni, Rossella Orlandi, Viviana Teresa Tettamanti, Gianluca Acquati, Francesco Grimaldi, Annalisa Int J Mol Sci Article Recent studies performed on the invertebrate model Hirudo verbana (medicinal leech) suggest that the T2 ribonucleic enzyme HvRNASET2 modulates the leech’s innate immune response, promoting microbial agglutination and supporting phagocytic cells recruitment in challenged tissues. Indeed, following injection of both lipoteichoic acid (LTA) and Staphylococcus aureus in the leech body wall, HvRNASET2 is expressed by leech type I granulocytes and induces bacterial aggregation to aid macrophage phagocytosis. Here, we investigate the HvRNASET2 antimicrobial role, in particular assessing the effects on the Gram-negative bacteria Escherichia coli. For this purpose, starting from the three-dimensional molecule reconstruction and in silico analyses, the antibacterial activity was evaluated both in vitro and in vivo. The changes induced in treated bacteria, such as agglutination and alteration in wall integrity, were observed by means of light, transmission and scanning electron microscopy. Moreover, immunogold, AMPs (antimicrobial peptides) and lipopolysaccharide (LPS) binding assays were carried out to evaluate HvRNASET2 interaction with the microbial envelopes and the ensuing ability to affect microbial viability. Finally, in vivo experiments confirmed that HvRNASET2 promotes a more rapid phagocytosis of bacterial aggregates by macrophages, representing a novel molecule for counteracting pathogen infections and developing alternative solutions to improve human health. MDPI 2020-12-19 /pmc/articles/PMC7767213/ /pubmed/33352806 http://dx.doi.org/10.3390/ijms21249722 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Baranzini, Nicolò Pulze, Laura Reguzzoni, Marcella Roncoroni, Rossella Orlandi, Viviana Teresa Tettamanti, Gianluca Acquati, Francesco Grimaldi, Annalisa 3D Reconstruction of HvRNASET2 Molecule to Understand Its Antibacterial Role |
title | 3D Reconstruction of HvRNASET2 Molecule to Understand Its Antibacterial Role |
title_full | 3D Reconstruction of HvRNASET2 Molecule to Understand Its Antibacterial Role |
title_fullStr | 3D Reconstruction of HvRNASET2 Molecule to Understand Its Antibacterial Role |
title_full_unstemmed | 3D Reconstruction of HvRNASET2 Molecule to Understand Its Antibacterial Role |
title_short | 3D Reconstruction of HvRNASET2 Molecule to Understand Its Antibacterial Role |
title_sort | 3d reconstruction of hvrnaset2 molecule to understand its antibacterial role |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7767213/ https://www.ncbi.nlm.nih.gov/pubmed/33352806 http://dx.doi.org/10.3390/ijms21249722 |
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