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3D Reconstruction of HvRNASET2 Molecule to Understand Its Antibacterial Role

Recent studies performed on the invertebrate model Hirudo verbana (medicinal leech) suggest that the T2 ribonucleic enzyme HvRNASET2 modulates the leech’s innate immune response, promoting microbial agglutination and supporting phagocytic cells recruitment in challenged tissues. Indeed, following in...

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Autores principales: Baranzini, Nicolò, Pulze, Laura, Reguzzoni, Marcella, Roncoroni, Rossella, Orlandi, Viviana Teresa, Tettamanti, Gianluca, Acquati, Francesco, Grimaldi, Annalisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7767213/
https://www.ncbi.nlm.nih.gov/pubmed/33352806
http://dx.doi.org/10.3390/ijms21249722
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author Baranzini, Nicolò
Pulze, Laura
Reguzzoni, Marcella
Roncoroni, Rossella
Orlandi, Viviana Teresa
Tettamanti, Gianluca
Acquati, Francesco
Grimaldi, Annalisa
author_facet Baranzini, Nicolò
Pulze, Laura
Reguzzoni, Marcella
Roncoroni, Rossella
Orlandi, Viviana Teresa
Tettamanti, Gianluca
Acquati, Francesco
Grimaldi, Annalisa
author_sort Baranzini, Nicolò
collection PubMed
description Recent studies performed on the invertebrate model Hirudo verbana (medicinal leech) suggest that the T2 ribonucleic enzyme HvRNASET2 modulates the leech’s innate immune response, promoting microbial agglutination and supporting phagocytic cells recruitment in challenged tissues. Indeed, following injection of both lipoteichoic acid (LTA) and Staphylococcus aureus in the leech body wall, HvRNASET2 is expressed by leech type I granulocytes and induces bacterial aggregation to aid macrophage phagocytosis. Here, we investigate the HvRNASET2 antimicrobial role, in particular assessing the effects on the Gram-negative bacteria Escherichia coli. For this purpose, starting from the three-dimensional molecule reconstruction and in silico analyses, the antibacterial activity was evaluated both in vitro and in vivo. The changes induced in treated bacteria, such as agglutination and alteration in wall integrity, were observed by means of light, transmission and scanning electron microscopy. Moreover, immunogold, AMPs (antimicrobial peptides) and lipopolysaccharide (LPS) binding assays were carried out to evaluate HvRNASET2 interaction with the microbial envelopes and the ensuing ability to affect microbial viability. Finally, in vivo experiments confirmed that HvRNASET2 promotes a more rapid phagocytosis of bacterial aggregates by macrophages, representing a novel molecule for counteracting pathogen infections and developing alternative solutions to improve human health.
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spelling pubmed-77672132020-12-28 3D Reconstruction of HvRNASET2 Molecule to Understand Its Antibacterial Role Baranzini, Nicolò Pulze, Laura Reguzzoni, Marcella Roncoroni, Rossella Orlandi, Viviana Teresa Tettamanti, Gianluca Acquati, Francesco Grimaldi, Annalisa Int J Mol Sci Article Recent studies performed on the invertebrate model Hirudo verbana (medicinal leech) suggest that the T2 ribonucleic enzyme HvRNASET2 modulates the leech’s innate immune response, promoting microbial agglutination and supporting phagocytic cells recruitment in challenged tissues. Indeed, following injection of both lipoteichoic acid (LTA) and Staphylococcus aureus in the leech body wall, HvRNASET2 is expressed by leech type I granulocytes and induces bacterial aggregation to aid macrophage phagocytosis. Here, we investigate the HvRNASET2 antimicrobial role, in particular assessing the effects on the Gram-negative bacteria Escherichia coli. For this purpose, starting from the three-dimensional molecule reconstruction and in silico analyses, the antibacterial activity was evaluated both in vitro and in vivo. The changes induced in treated bacteria, such as agglutination and alteration in wall integrity, were observed by means of light, transmission and scanning electron microscopy. Moreover, immunogold, AMPs (antimicrobial peptides) and lipopolysaccharide (LPS) binding assays were carried out to evaluate HvRNASET2 interaction with the microbial envelopes and the ensuing ability to affect microbial viability. Finally, in vivo experiments confirmed that HvRNASET2 promotes a more rapid phagocytosis of bacterial aggregates by macrophages, representing a novel molecule for counteracting pathogen infections and developing alternative solutions to improve human health. MDPI 2020-12-19 /pmc/articles/PMC7767213/ /pubmed/33352806 http://dx.doi.org/10.3390/ijms21249722 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Baranzini, Nicolò
Pulze, Laura
Reguzzoni, Marcella
Roncoroni, Rossella
Orlandi, Viviana Teresa
Tettamanti, Gianluca
Acquati, Francesco
Grimaldi, Annalisa
3D Reconstruction of HvRNASET2 Molecule to Understand Its Antibacterial Role
title 3D Reconstruction of HvRNASET2 Molecule to Understand Its Antibacterial Role
title_full 3D Reconstruction of HvRNASET2 Molecule to Understand Its Antibacterial Role
title_fullStr 3D Reconstruction of HvRNASET2 Molecule to Understand Its Antibacterial Role
title_full_unstemmed 3D Reconstruction of HvRNASET2 Molecule to Understand Its Antibacterial Role
title_short 3D Reconstruction of HvRNASET2 Molecule to Understand Its Antibacterial Role
title_sort 3d reconstruction of hvrnaset2 molecule to understand its antibacterial role
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7767213/
https://www.ncbi.nlm.nih.gov/pubmed/33352806
http://dx.doi.org/10.3390/ijms21249722
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