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Assessment of Endothelial Injury and Pro-Coagulant Activity Using Circulating Microvesicles in Survivors of Allogeneic Hematopoietic Cell Transplantation

(1) Background: survivors of allogeneic hematopoietic cell transplantation (alloHCT) suffer from morbidity and mortality due to cardiovascular events. We hypothesized that vascular injury and pro-coagulant activity are evident in alloHCT survivors without existing alloHCT complications or relapse. (...

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Autores principales: Gavriilaki, Eleni, Sakellari, Ioanna, Anyfanti, Panagiota, Batsis, Ioannis, Vardi, Anna, Bousiou, Zoi, Lazaridis, Antonios, Nikolaidou, Barbara, Zarifis, Ippokratis, Masmanidou, Marianna, Yiannaki, Efthalia, Markala, Dimitra, Anagnostopoulos, Achilles, Douma, Stella, Gkaliagkousi, Eugenia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7767425/
https://www.ncbi.nlm.nih.gov/pubmed/33371421
http://dx.doi.org/10.3390/ijms21249768
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author Gavriilaki, Eleni
Sakellari, Ioanna
Anyfanti, Panagiota
Batsis, Ioannis
Vardi, Anna
Bousiou, Zoi
Lazaridis, Antonios
Nikolaidou, Barbara
Zarifis, Ippokratis
Masmanidou, Marianna
Yiannaki, Efthalia
Markala, Dimitra
Anagnostopoulos, Achilles
Douma, Stella
Gkaliagkousi, Eugenia
author_facet Gavriilaki, Eleni
Sakellari, Ioanna
Anyfanti, Panagiota
Batsis, Ioannis
Vardi, Anna
Bousiou, Zoi
Lazaridis, Antonios
Nikolaidou, Barbara
Zarifis, Ippokratis
Masmanidou, Marianna
Yiannaki, Efthalia
Markala, Dimitra
Anagnostopoulos, Achilles
Douma, Stella
Gkaliagkousi, Eugenia
author_sort Gavriilaki, Eleni
collection PubMed
description (1) Background: survivors of allogeneic hematopoietic cell transplantation (alloHCT) suffer from morbidity and mortality due to cardiovascular events. We hypothesized that vascular injury and pro-coagulant activity are evident in alloHCT survivors without existing alloHCT complications or relapse. (2) Methods: we enrolled consecutive adult alloHCT survivors without established cardiovascular disease and control individuals matched for traditional cardiovascular risk factors (January–December 2019). Circulating microvesicles (MVs) of different cellular origins (platelet, erythrocyte, and endothelial) were measured by a standardized flow cytometry protocol as novel markers of vascular injury and pro-coagulant activity. (3) Results: we recruited 45 survivors after a median of 2.3 (range 1.1–13.2) years from alloHCT, and 45 controls. The majority of patients suffered from acute (44%) and/or chronic (66%) graft-versus-host disease (GVHD). Although the two groups were matched for traditional cardiovascular risk factors, alloHCT survivors showed significantly increased platelet and erythrocyte MVs compared to controls. Within alloHCT survivors, erythrocyte MVs were significantly increased in patients with a previous history of thrombotic microangiopathy. Interestingly, endothelial MVs were significantly increased only in alloHCT recipients of a myeloablative conditioning. Furthermore, MVs of different origins showed a positive association with each other. (4) Conclusions: endothelial dysfunction and increased thrombotic risk are evident in alloHCT recipients long after alloHCT, independently of traditional cardiovascular risk factors. An apparent synergism of these pathophysiological processes may be strongly involved in the subsequent establishment of cardiovascular disease.
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spelling pubmed-77674252020-12-28 Assessment of Endothelial Injury and Pro-Coagulant Activity Using Circulating Microvesicles in Survivors of Allogeneic Hematopoietic Cell Transplantation Gavriilaki, Eleni Sakellari, Ioanna Anyfanti, Panagiota Batsis, Ioannis Vardi, Anna Bousiou, Zoi Lazaridis, Antonios Nikolaidou, Barbara Zarifis, Ippokratis Masmanidou, Marianna Yiannaki, Efthalia Markala, Dimitra Anagnostopoulos, Achilles Douma, Stella Gkaliagkousi, Eugenia Int J Mol Sci Article (1) Background: survivors of allogeneic hematopoietic cell transplantation (alloHCT) suffer from morbidity and mortality due to cardiovascular events. We hypothesized that vascular injury and pro-coagulant activity are evident in alloHCT survivors without existing alloHCT complications or relapse. (2) Methods: we enrolled consecutive adult alloHCT survivors without established cardiovascular disease and control individuals matched for traditional cardiovascular risk factors (January–December 2019). Circulating microvesicles (MVs) of different cellular origins (platelet, erythrocyte, and endothelial) were measured by a standardized flow cytometry protocol as novel markers of vascular injury and pro-coagulant activity. (3) Results: we recruited 45 survivors after a median of 2.3 (range 1.1–13.2) years from alloHCT, and 45 controls. The majority of patients suffered from acute (44%) and/or chronic (66%) graft-versus-host disease (GVHD). Although the two groups were matched for traditional cardiovascular risk factors, alloHCT survivors showed significantly increased platelet and erythrocyte MVs compared to controls. Within alloHCT survivors, erythrocyte MVs were significantly increased in patients with a previous history of thrombotic microangiopathy. Interestingly, endothelial MVs were significantly increased only in alloHCT recipients of a myeloablative conditioning. Furthermore, MVs of different origins showed a positive association with each other. (4) Conclusions: endothelial dysfunction and increased thrombotic risk are evident in alloHCT recipients long after alloHCT, independently of traditional cardiovascular risk factors. An apparent synergism of these pathophysiological processes may be strongly involved in the subsequent establishment of cardiovascular disease. MDPI 2020-12-21 /pmc/articles/PMC7767425/ /pubmed/33371421 http://dx.doi.org/10.3390/ijms21249768 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gavriilaki, Eleni
Sakellari, Ioanna
Anyfanti, Panagiota
Batsis, Ioannis
Vardi, Anna
Bousiou, Zoi
Lazaridis, Antonios
Nikolaidou, Barbara
Zarifis, Ippokratis
Masmanidou, Marianna
Yiannaki, Efthalia
Markala, Dimitra
Anagnostopoulos, Achilles
Douma, Stella
Gkaliagkousi, Eugenia
Assessment of Endothelial Injury and Pro-Coagulant Activity Using Circulating Microvesicles in Survivors of Allogeneic Hematopoietic Cell Transplantation
title Assessment of Endothelial Injury and Pro-Coagulant Activity Using Circulating Microvesicles in Survivors of Allogeneic Hematopoietic Cell Transplantation
title_full Assessment of Endothelial Injury and Pro-Coagulant Activity Using Circulating Microvesicles in Survivors of Allogeneic Hematopoietic Cell Transplantation
title_fullStr Assessment of Endothelial Injury and Pro-Coagulant Activity Using Circulating Microvesicles in Survivors of Allogeneic Hematopoietic Cell Transplantation
title_full_unstemmed Assessment of Endothelial Injury and Pro-Coagulant Activity Using Circulating Microvesicles in Survivors of Allogeneic Hematopoietic Cell Transplantation
title_short Assessment of Endothelial Injury and Pro-Coagulant Activity Using Circulating Microvesicles in Survivors of Allogeneic Hematopoietic Cell Transplantation
title_sort assessment of endothelial injury and pro-coagulant activity using circulating microvesicles in survivors of allogeneic hematopoietic cell transplantation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7767425/
https://www.ncbi.nlm.nih.gov/pubmed/33371421
http://dx.doi.org/10.3390/ijms21249768
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