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Molecular Characterization of Staphylococcus aureus Isolated from Chronic Infected Wounds in Rural Ghana

Background: Globally, Staphylococcus aureus is an important bacterial pathogen causing a wide range of community and hospital acquired infections. In Ghana, resistance of S. aureus to locally available antibiotics is increasing but the molecular basis of resistance and the population structure of S....

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Autores principales: Wolters, Manuel, Frickmann, Hagen, Christner, Martin, Both, Anna, Rohde, Holger, Oppong, Kwabena, Akenten, Charity Wiafe, May, Jürgen, Dekker, Denise
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7767444/
https://www.ncbi.nlm.nih.gov/pubmed/33371449
http://dx.doi.org/10.3390/microorganisms8122052
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author Wolters, Manuel
Frickmann, Hagen
Christner, Martin
Both, Anna
Rohde, Holger
Oppong, Kwabena
Akenten, Charity Wiafe
May, Jürgen
Dekker, Denise
author_facet Wolters, Manuel
Frickmann, Hagen
Christner, Martin
Both, Anna
Rohde, Holger
Oppong, Kwabena
Akenten, Charity Wiafe
May, Jürgen
Dekker, Denise
author_sort Wolters, Manuel
collection PubMed
description Background: Globally, Staphylococcus aureus is an important bacterial pathogen causing a wide range of community and hospital acquired infections. In Ghana, resistance of S. aureus to locally available antibiotics is increasing but the molecular basis of resistance and the population structure of S. aureus in particular in chronic wounds are poorly described. However, this information is essential to understand the underlying mechanisms of resistance and spread of resistant clones. We therefore subjected 28 S. aureus isolates from chronic infected wounds in a rural area of Ghana to whole genome sequencing. Results: Overall, resistance of S. aureus to locally available antibiotics was high and 29% were Methicillin resistant Staphylococcus aureus (MRSA). The most abundant sequence type was ST88 (29%, 8/28) followed by ST152 (18%, 5/28). All ST88 carried the mecA gene, which was associated with this sequence type only. Chloramphenicol resistance gene fexB was exclusively associated with the methicillin-resistant ST88 strains. Panton-Valentine leukocidin (PVL) carriage was associated with ST121 and ST152. Other detected mechanisms of resistance included dfrG, conferring resistance to trimethoprim. Conclusions: This study provides valuable information for understanding the population structure and resistance mechanisms of S. aureus isolated from chronic wound infections in rural Ghana.
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spelling pubmed-77674442020-12-28 Molecular Characterization of Staphylococcus aureus Isolated from Chronic Infected Wounds in Rural Ghana Wolters, Manuel Frickmann, Hagen Christner, Martin Both, Anna Rohde, Holger Oppong, Kwabena Akenten, Charity Wiafe May, Jürgen Dekker, Denise Microorganisms Communication Background: Globally, Staphylococcus aureus is an important bacterial pathogen causing a wide range of community and hospital acquired infections. In Ghana, resistance of S. aureus to locally available antibiotics is increasing but the molecular basis of resistance and the population structure of S. aureus in particular in chronic wounds are poorly described. However, this information is essential to understand the underlying mechanisms of resistance and spread of resistant clones. We therefore subjected 28 S. aureus isolates from chronic infected wounds in a rural area of Ghana to whole genome sequencing. Results: Overall, resistance of S. aureus to locally available antibiotics was high and 29% were Methicillin resistant Staphylococcus aureus (MRSA). The most abundant sequence type was ST88 (29%, 8/28) followed by ST152 (18%, 5/28). All ST88 carried the mecA gene, which was associated with this sequence type only. Chloramphenicol resistance gene fexB was exclusively associated with the methicillin-resistant ST88 strains. Panton-Valentine leukocidin (PVL) carriage was associated with ST121 and ST152. Other detected mechanisms of resistance included dfrG, conferring resistance to trimethoprim. Conclusions: This study provides valuable information for understanding the population structure and resistance mechanisms of S. aureus isolated from chronic wound infections in rural Ghana. MDPI 2020-12-21 /pmc/articles/PMC7767444/ /pubmed/33371449 http://dx.doi.org/10.3390/microorganisms8122052 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Wolters, Manuel
Frickmann, Hagen
Christner, Martin
Both, Anna
Rohde, Holger
Oppong, Kwabena
Akenten, Charity Wiafe
May, Jürgen
Dekker, Denise
Molecular Characterization of Staphylococcus aureus Isolated from Chronic Infected Wounds in Rural Ghana
title Molecular Characterization of Staphylococcus aureus Isolated from Chronic Infected Wounds in Rural Ghana
title_full Molecular Characterization of Staphylococcus aureus Isolated from Chronic Infected Wounds in Rural Ghana
title_fullStr Molecular Characterization of Staphylococcus aureus Isolated from Chronic Infected Wounds in Rural Ghana
title_full_unstemmed Molecular Characterization of Staphylococcus aureus Isolated from Chronic Infected Wounds in Rural Ghana
title_short Molecular Characterization of Staphylococcus aureus Isolated from Chronic Infected Wounds in Rural Ghana
title_sort molecular characterization of staphylococcus aureus isolated from chronic infected wounds in rural ghana
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7767444/
https://www.ncbi.nlm.nih.gov/pubmed/33371449
http://dx.doi.org/10.3390/microorganisms8122052
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