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Thermal Inactivation of Different Capripox Virus Isolates

Capripox viruses (CaPVs) cause a highly contagious poxvirus disease of livestock animals. Working with CaPVs requires laboratories with a high biosecurity level (BSL 3), and reliable inactivation of these viruses is therefore necessary for working in areas or laboratories with a lower biosecurity st...

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Autores principales: Wolff, Janika, Beer, Martin, Hoffmann, Bernd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7767500/
https://www.ncbi.nlm.nih.gov/pubmed/33371463
http://dx.doi.org/10.3390/microorganisms8122053
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author Wolff, Janika
Beer, Martin
Hoffmann, Bernd
author_facet Wolff, Janika
Beer, Martin
Hoffmann, Bernd
author_sort Wolff, Janika
collection PubMed
description Capripox viruses (CaPVs) cause a highly contagious poxvirus disease of livestock animals. Working with CaPVs requires laboratories with a high biosecurity level (BSL 3), and reliable inactivation of these viruses is therefore necessary for working in areas or laboratories with a lower biosecurity status. Heat treatment provides a simple and well-established tool for the inactivation due to its substantial advantages (e.g., easy to perform, fast, cheap, and robust). In our study, we determined the time–temperature profiles needed for a fail-safe inactivation procedure using four different CaPV isolates in aqueous solution with and without the addition of protective serum. All four tested CaPV isolates were completely inactivated after 30 min at 56 °C or 10 min at 60 °C. Since different thermal stabilities of other CaPV isolates could not be fully excluded, we recommend an inactivation procedure of 1 h at 56 °C for safe shipment or working in laboratories with lower biosecurity levels than BSL 3.
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spelling pubmed-77675002020-12-28 Thermal Inactivation of Different Capripox Virus Isolates Wolff, Janika Beer, Martin Hoffmann, Bernd Microorganisms Communication Capripox viruses (CaPVs) cause a highly contagious poxvirus disease of livestock animals. Working with CaPVs requires laboratories with a high biosecurity level (BSL 3), and reliable inactivation of these viruses is therefore necessary for working in areas or laboratories with a lower biosecurity status. Heat treatment provides a simple and well-established tool for the inactivation due to its substantial advantages (e.g., easy to perform, fast, cheap, and robust). In our study, we determined the time–temperature profiles needed for a fail-safe inactivation procedure using four different CaPV isolates in aqueous solution with and without the addition of protective serum. All four tested CaPV isolates were completely inactivated after 30 min at 56 °C or 10 min at 60 °C. Since different thermal stabilities of other CaPV isolates could not be fully excluded, we recommend an inactivation procedure of 1 h at 56 °C for safe shipment or working in laboratories with lower biosecurity levels than BSL 3. MDPI 2020-12-21 /pmc/articles/PMC7767500/ /pubmed/33371463 http://dx.doi.org/10.3390/microorganisms8122053 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Wolff, Janika
Beer, Martin
Hoffmann, Bernd
Thermal Inactivation of Different Capripox Virus Isolates
title Thermal Inactivation of Different Capripox Virus Isolates
title_full Thermal Inactivation of Different Capripox Virus Isolates
title_fullStr Thermal Inactivation of Different Capripox Virus Isolates
title_full_unstemmed Thermal Inactivation of Different Capripox Virus Isolates
title_short Thermal Inactivation of Different Capripox Virus Isolates
title_sort thermal inactivation of different capripox virus isolates
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7767500/
https://www.ncbi.nlm.nih.gov/pubmed/33371463
http://dx.doi.org/10.3390/microorganisms8122053
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