Cargando…

Evaluation of Analgesic and Anti-inflammatory Potential of 80% Methanol Leaf Extract of Otostegia integrifolia Benth (Lamiaceae)

INTRODUCTION: Pain and inflammatory disorders are the most prevalent syndromes. Different herbs were used for treatment of pain and inflammation including members of the genus Otostegia. As a result, this research investigated the in vivo analgesic and anti-inflammatory effects of 80% methanol leaf...

Descripción completa

Detalles Bibliográficos
Autores principales: Tesfaye, Rediet, Degu, Abel, Abebe, Besufekad, Ayalew, Hiwot
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7767723/
https://www.ncbi.nlm.nih.gov/pubmed/33380820
http://dx.doi.org/10.2147/JIR.S285932
_version_ 1783629023012716544
author Tesfaye, Rediet
Degu, Abel
Abebe, Besufekad
Ayalew, Hiwot
author_facet Tesfaye, Rediet
Degu, Abel
Abebe, Besufekad
Ayalew, Hiwot
author_sort Tesfaye, Rediet
collection PubMed
description INTRODUCTION: Pain and inflammatory disorders are the most prevalent syndromes. Different herbs were used for treatment of pain and inflammation including members of the genus Otostegia. As a result, this research investigated the in vivo analgesic and anti-inflammatory effects of 80% methanol leaf extract of Otostegia integrifolia in mice. METHODS: The analgesic and anti-inflammatory effects of the plant was evaluated using hot plate method, acetic-acid induced writhing test, and carrageenan and formalin induced paw edema. Three experimental groups (100, 200, and 400 mg/kg) received the extract while morphine 10 mg/kg and aspirin 150 mg/kg were used as a positive control for analgesic and anti-inflammatory tests accordingly. Distilled water (10 mL/kg) was used as negative control. RESULTS: From the experimental groups, OI400 displayed significant analgesic and anti-inflammatory activities (P<0.001). In acetic acid induced writhing tests, the number of writhes decreased significantly (P<0.001) in all experimental groups. Similarly, OI400 reduced the mean paw edema significantly in carrageenan and formalin induced paw edema (P<0.05 and P<0.001, respectively). CONCLUSION: In general, the results obtained in this study demonstrated that the extract exhibited significant analgesic and anti-inflammatory potential in mice.
format Online
Article
Text
id pubmed-7767723
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Dove
record_format MEDLINE/PubMed
spelling pubmed-77677232020-12-29 Evaluation of Analgesic and Anti-inflammatory Potential of 80% Methanol Leaf Extract of Otostegia integrifolia Benth (Lamiaceae) Tesfaye, Rediet Degu, Abel Abebe, Besufekad Ayalew, Hiwot J Inflamm Res Original Research INTRODUCTION: Pain and inflammatory disorders are the most prevalent syndromes. Different herbs were used for treatment of pain and inflammation including members of the genus Otostegia. As a result, this research investigated the in vivo analgesic and anti-inflammatory effects of 80% methanol leaf extract of Otostegia integrifolia in mice. METHODS: The analgesic and anti-inflammatory effects of the plant was evaluated using hot plate method, acetic-acid induced writhing test, and carrageenan and formalin induced paw edema. Three experimental groups (100, 200, and 400 mg/kg) received the extract while morphine 10 mg/kg and aspirin 150 mg/kg were used as a positive control for analgesic and anti-inflammatory tests accordingly. Distilled water (10 mL/kg) was used as negative control. RESULTS: From the experimental groups, OI400 displayed significant analgesic and anti-inflammatory activities (P<0.001). In acetic acid induced writhing tests, the number of writhes decreased significantly (P<0.001) in all experimental groups. Similarly, OI400 reduced the mean paw edema significantly in carrageenan and formalin induced paw edema (P<0.05 and P<0.001, respectively). CONCLUSION: In general, the results obtained in this study demonstrated that the extract exhibited significant analgesic and anti-inflammatory potential in mice. Dove 2020-12-23 /pmc/articles/PMC7767723/ /pubmed/33380820 http://dx.doi.org/10.2147/JIR.S285932 Text en © 2020 Tesfaye et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Tesfaye, Rediet
Degu, Abel
Abebe, Besufekad
Ayalew, Hiwot
Evaluation of Analgesic and Anti-inflammatory Potential of 80% Methanol Leaf Extract of Otostegia integrifolia Benth (Lamiaceae)
title Evaluation of Analgesic and Anti-inflammatory Potential of 80% Methanol Leaf Extract of Otostegia integrifolia Benth (Lamiaceae)
title_full Evaluation of Analgesic and Anti-inflammatory Potential of 80% Methanol Leaf Extract of Otostegia integrifolia Benth (Lamiaceae)
title_fullStr Evaluation of Analgesic and Anti-inflammatory Potential of 80% Methanol Leaf Extract of Otostegia integrifolia Benth (Lamiaceae)
title_full_unstemmed Evaluation of Analgesic and Anti-inflammatory Potential of 80% Methanol Leaf Extract of Otostegia integrifolia Benth (Lamiaceae)
title_short Evaluation of Analgesic and Anti-inflammatory Potential of 80% Methanol Leaf Extract of Otostegia integrifolia Benth (Lamiaceae)
title_sort evaluation of analgesic and anti-inflammatory potential of 80% methanol leaf extract of otostegia integrifolia benth (lamiaceae)
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7767723/
https://www.ncbi.nlm.nih.gov/pubmed/33380820
http://dx.doi.org/10.2147/JIR.S285932
work_keys_str_mv AT tesfayerediet evaluationofanalgesicandantiinflammatorypotentialof80methanolleafextractofotostegiaintegrifoliabenthlamiaceae
AT deguabel evaluationofanalgesicandantiinflammatorypotentialof80methanolleafextractofotostegiaintegrifoliabenthlamiaceae
AT abebebesufekad evaluationofanalgesicandantiinflammatorypotentialof80methanolleafextractofotostegiaintegrifoliabenthlamiaceae
AT ayalewhiwot evaluationofanalgesicandantiinflammatorypotentialof80methanolleafextractofotostegiaintegrifoliabenthlamiaceae