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Evaluation of Analgesic and Anti-inflammatory Potential of 80% Methanol Leaf Extract of Otostegia integrifolia Benth (Lamiaceae)
INTRODUCTION: Pain and inflammatory disorders are the most prevalent syndromes. Different herbs were used for treatment of pain and inflammation including members of the genus Otostegia. As a result, this research investigated the in vivo analgesic and anti-inflammatory effects of 80% methanol leaf...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7767723/ https://www.ncbi.nlm.nih.gov/pubmed/33380820 http://dx.doi.org/10.2147/JIR.S285932 |
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author | Tesfaye, Rediet Degu, Abel Abebe, Besufekad Ayalew, Hiwot |
author_facet | Tesfaye, Rediet Degu, Abel Abebe, Besufekad Ayalew, Hiwot |
author_sort | Tesfaye, Rediet |
collection | PubMed |
description | INTRODUCTION: Pain and inflammatory disorders are the most prevalent syndromes. Different herbs were used for treatment of pain and inflammation including members of the genus Otostegia. As a result, this research investigated the in vivo analgesic and anti-inflammatory effects of 80% methanol leaf extract of Otostegia integrifolia in mice. METHODS: The analgesic and anti-inflammatory effects of the plant was evaluated using hot plate method, acetic-acid induced writhing test, and carrageenan and formalin induced paw edema. Three experimental groups (100, 200, and 400 mg/kg) received the extract while morphine 10 mg/kg and aspirin 150 mg/kg were used as a positive control for analgesic and anti-inflammatory tests accordingly. Distilled water (10 mL/kg) was used as negative control. RESULTS: From the experimental groups, OI400 displayed significant analgesic and anti-inflammatory activities (P<0.001). In acetic acid induced writhing tests, the number of writhes decreased significantly (P<0.001) in all experimental groups. Similarly, OI400 reduced the mean paw edema significantly in carrageenan and formalin induced paw edema (P<0.05 and P<0.001, respectively). CONCLUSION: In general, the results obtained in this study demonstrated that the extract exhibited significant analgesic and anti-inflammatory potential in mice. |
format | Online Article Text |
id | pubmed-7767723 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-77677232020-12-29 Evaluation of Analgesic and Anti-inflammatory Potential of 80% Methanol Leaf Extract of Otostegia integrifolia Benth (Lamiaceae) Tesfaye, Rediet Degu, Abel Abebe, Besufekad Ayalew, Hiwot J Inflamm Res Original Research INTRODUCTION: Pain and inflammatory disorders are the most prevalent syndromes. Different herbs were used for treatment of pain and inflammation including members of the genus Otostegia. As a result, this research investigated the in vivo analgesic and anti-inflammatory effects of 80% methanol leaf extract of Otostegia integrifolia in mice. METHODS: The analgesic and anti-inflammatory effects of the plant was evaluated using hot plate method, acetic-acid induced writhing test, and carrageenan and formalin induced paw edema. Three experimental groups (100, 200, and 400 mg/kg) received the extract while morphine 10 mg/kg and aspirin 150 mg/kg were used as a positive control for analgesic and anti-inflammatory tests accordingly. Distilled water (10 mL/kg) was used as negative control. RESULTS: From the experimental groups, OI400 displayed significant analgesic and anti-inflammatory activities (P<0.001). In acetic acid induced writhing tests, the number of writhes decreased significantly (P<0.001) in all experimental groups. Similarly, OI400 reduced the mean paw edema significantly in carrageenan and formalin induced paw edema (P<0.05 and P<0.001, respectively). CONCLUSION: In general, the results obtained in this study demonstrated that the extract exhibited significant analgesic and anti-inflammatory potential in mice. Dove 2020-12-23 /pmc/articles/PMC7767723/ /pubmed/33380820 http://dx.doi.org/10.2147/JIR.S285932 Text en © 2020 Tesfaye et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Tesfaye, Rediet Degu, Abel Abebe, Besufekad Ayalew, Hiwot Evaluation of Analgesic and Anti-inflammatory Potential of 80% Methanol Leaf Extract of Otostegia integrifolia Benth (Lamiaceae) |
title | Evaluation of Analgesic and Anti-inflammatory Potential of 80% Methanol Leaf Extract of Otostegia integrifolia Benth (Lamiaceae) |
title_full | Evaluation of Analgesic and Anti-inflammatory Potential of 80% Methanol Leaf Extract of Otostegia integrifolia Benth (Lamiaceae) |
title_fullStr | Evaluation of Analgesic and Anti-inflammatory Potential of 80% Methanol Leaf Extract of Otostegia integrifolia Benth (Lamiaceae) |
title_full_unstemmed | Evaluation of Analgesic and Anti-inflammatory Potential of 80% Methanol Leaf Extract of Otostegia integrifolia Benth (Lamiaceae) |
title_short | Evaluation of Analgesic and Anti-inflammatory Potential of 80% Methanol Leaf Extract of Otostegia integrifolia Benth (Lamiaceae) |
title_sort | evaluation of analgesic and anti-inflammatory potential of 80% methanol leaf extract of otostegia integrifolia benth (lamiaceae) |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7767723/ https://www.ncbi.nlm.nih.gov/pubmed/33380820 http://dx.doi.org/10.2147/JIR.S285932 |
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