Cargando…
A Serum Metabolic Profiling Analysis During the Formation of Fatty Liver in Landes Geese via GC-TOF/MS
During the process of fatty liver production by overfeeding, the levels of endogenous metabolites in the serum of geese would change dramatically. This study investigated the effects of overfeeding on serum metabolism of Landes geese and the underlying mechanisms using a metabolomics approach. Sixty...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7767842/ https://www.ncbi.nlm.nih.gov/pubmed/33381050 http://dx.doi.org/10.3389/fphys.2020.581699 |
_version_ | 1783629050472824832 |
---|---|
author | Gong, Yujie Lyu, Wentao Shi, Xingfen Zou, Xiaoting Lu, Lizhi Yang, Hua Xiao, Yingping |
author_facet | Gong, Yujie Lyu, Wentao Shi, Xingfen Zou, Xiaoting Lu, Lizhi Yang, Hua Xiao, Yingping |
author_sort | Gong, Yujie |
collection | PubMed |
description | During the process of fatty liver production by overfeeding, the levels of endogenous metabolites in the serum of geese would change dramatically. This study investigated the effects of overfeeding on serum metabolism of Landes geese and the underlying mechanisms using a metabolomics approach. Sixty Landes geese of the same age were randomly divided into the following three groups with 20 replicates in each group: D0 group (free from gavage); D7 group (overfeeding for 7 days); D25 group (overfeeding for 25 days). At the end of the experiment, 10 geese of similar weight from each group were selected for slaughter and sampling. The results showed that overfeeding significantly increased the body weight and the liver weight of geese. Serum enzymatic activities and serum lipid levels were significantly enhanced following overfeeding. Gas chromatography time-of-flight/mass spectrometry (GC-TOF/MS) was employed to explore the serum metabolic patterns, and to identify potential contributors to the formation of fatty liver and the correlated metabolic pathways. Relative to overfeeding for 7 days, a large number of endogenous molecules in serum of geese overfed for 25 days were altered. Continuous elevated levels of pyruvic acid, alanine, proline and beta-glycerophosphoric acid and reduced lactic acid level were observed in the serum of overfed geese. Pathway exploration found that the most of significantly different metabolites were involved in amino acids, carbohydrate and lipid metabolism. The present study exhibited the efficient capability of Landes geese to produce fatty liver, identified potential biomarkers and disturbed metabolic pathways in liver steatosis. These findings might reveal the underlying mechanisms of fatty liver formation and provide some theoretical basis for the diagnosis and treatment of liver diseases. |
format | Online Article Text |
id | pubmed-7767842 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-77678422020-12-29 A Serum Metabolic Profiling Analysis During the Formation of Fatty Liver in Landes Geese via GC-TOF/MS Gong, Yujie Lyu, Wentao Shi, Xingfen Zou, Xiaoting Lu, Lizhi Yang, Hua Xiao, Yingping Front Physiol Physiology During the process of fatty liver production by overfeeding, the levels of endogenous metabolites in the serum of geese would change dramatically. This study investigated the effects of overfeeding on serum metabolism of Landes geese and the underlying mechanisms using a metabolomics approach. Sixty Landes geese of the same age were randomly divided into the following three groups with 20 replicates in each group: D0 group (free from gavage); D7 group (overfeeding for 7 days); D25 group (overfeeding for 25 days). At the end of the experiment, 10 geese of similar weight from each group were selected for slaughter and sampling. The results showed that overfeeding significantly increased the body weight and the liver weight of geese. Serum enzymatic activities and serum lipid levels were significantly enhanced following overfeeding. Gas chromatography time-of-flight/mass spectrometry (GC-TOF/MS) was employed to explore the serum metabolic patterns, and to identify potential contributors to the formation of fatty liver and the correlated metabolic pathways. Relative to overfeeding for 7 days, a large number of endogenous molecules in serum of geese overfed for 25 days were altered. Continuous elevated levels of pyruvic acid, alanine, proline and beta-glycerophosphoric acid and reduced lactic acid level were observed in the serum of overfed geese. Pathway exploration found that the most of significantly different metabolites were involved in amino acids, carbohydrate and lipid metabolism. The present study exhibited the efficient capability of Landes geese to produce fatty liver, identified potential biomarkers and disturbed metabolic pathways in liver steatosis. These findings might reveal the underlying mechanisms of fatty liver formation and provide some theoretical basis for the diagnosis and treatment of liver diseases. Frontiers Media S.A. 2020-12-14 /pmc/articles/PMC7767842/ /pubmed/33381050 http://dx.doi.org/10.3389/fphys.2020.581699 Text en Copyright © 2020 Gong, Lyu, Shi, Zou, Lu, Yang and Xiao. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology Gong, Yujie Lyu, Wentao Shi, Xingfen Zou, Xiaoting Lu, Lizhi Yang, Hua Xiao, Yingping A Serum Metabolic Profiling Analysis During the Formation of Fatty Liver in Landes Geese via GC-TOF/MS |
title | A Serum Metabolic Profiling Analysis During the Formation of Fatty Liver in Landes Geese via GC-TOF/MS |
title_full | A Serum Metabolic Profiling Analysis During the Formation of Fatty Liver in Landes Geese via GC-TOF/MS |
title_fullStr | A Serum Metabolic Profiling Analysis During the Formation of Fatty Liver in Landes Geese via GC-TOF/MS |
title_full_unstemmed | A Serum Metabolic Profiling Analysis During the Formation of Fatty Liver in Landes Geese via GC-TOF/MS |
title_short | A Serum Metabolic Profiling Analysis During the Formation of Fatty Liver in Landes Geese via GC-TOF/MS |
title_sort | serum metabolic profiling analysis during the formation of fatty liver in landes geese via gc-tof/ms |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7767842/ https://www.ncbi.nlm.nih.gov/pubmed/33381050 http://dx.doi.org/10.3389/fphys.2020.581699 |
work_keys_str_mv | AT gongyujie aserummetabolicprofilinganalysisduringtheformationoffattyliverinlandesgeeseviagctofms AT lyuwentao aserummetabolicprofilinganalysisduringtheformationoffattyliverinlandesgeeseviagctofms AT shixingfen aserummetabolicprofilinganalysisduringtheformationoffattyliverinlandesgeeseviagctofms AT zouxiaoting aserummetabolicprofilinganalysisduringtheformationoffattyliverinlandesgeeseviagctofms AT lulizhi aserummetabolicprofilinganalysisduringtheformationoffattyliverinlandesgeeseviagctofms AT yanghua aserummetabolicprofilinganalysisduringtheformationoffattyliverinlandesgeeseviagctofms AT xiaoyingping aserummetabolicprofilinganalysisduringtheformationoffattyliverinlandesgeeseviagctofms AT gongyujie serummetabolicprofilinganalysisduringtheformationoffattyliverinlandesgeeseviagctofms AT lyuwentao serummetabolicprofilinganalysisduringtheformationoffattyliverinlandesgeeseviagctofms AT shixingfen serummetabolicprofilinganalysisduringtheformationoffattyliverinlandesgeeseviagctofms AT zouxiaoting serummetabolicprofilinganalysisduringtheformationoffattyliverinlandesgeeseviagctofms AT lulizhi serummetabolicprofilinganalysisduringtheformationoffattyliverinlandesgeeseviagctofms AT yanghua serummetabolicprofilinganalysisduringtheformationoffattyliverinlandesgeeseviagctofms AT xiaoyingping serummetabolicprofilinganalysisduringtheformationoffattyliverinlandesgeeseviagctofms |