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Decreased Colonic Guanylin/Uroguanylin Expression and Dried Stool Property in Mice With Social Defeat Stress

Psychological stress is deeply involved in the pathophysiology of not only mental illness but also functional gastrointestinal disorders. In the present study, we examined the relationship between psychological stress and abnormality of stool properties, focusing on the alteration of plasma glucocor...

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Autores principales: Ebisutani, Nobuhiko, Fukui, Hirokazu, Nishimura, Heihachiro, Nakanishi, Takashi, Morimoto, Kenki, Itou, Shiho, Nakamura, Ayumi, Masutani, Mizuki, Hori, Mika, Tomita, Toshihiko, Oshima, Tadayuki, Kasahara, Emiko, Sekiyama, Atsuo, Miwa, Hiroto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7767843/
https://www.ncbi.nlm.nih.gov/pubmed/33381053
http://dx.doi.org/10.3389/fphys.2020.599582
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author Ebisutani, Nobuhiko
Fukui, Hirokazu
Nishimura, Heihachiro
Nakanishi, Takashi
Morimoto, Kenki
Itou, Shiho
Nakamura, Ayumi
Masutani, Mizuki
Hori, Mika
Tomita, Toshihiko
Oshima, Tadayuki
Kasahara, Emiko
Sekiyama, Atsuo
Miwa, Hiroto
author_facet Ebisutani, Nobuhiko
Fukui, Hirokazu
Nishimura, Heihachiro
Nakanishi, Takashi
Morimoto, Kenki
Itou, Shiho
Nakamura, Ayumi
Masutani, Mizuki
Hori, Mika
Tomita, Toshihiko
Oshima, Tadayuki
Kasahara, Emiko
Sekiyama, Atsuo
Miwa, Hiroto
author_sort Ebisutani, Nobuhiko
collection PubMed
description Psychological stress is deeply involved in the pathophysiology of not only mental illness but also functional gastrointestinal disorders. In the present study, we examined the relationship between psychological stress and abnormality of stool properties, focusing on the alteration of plasma glucocorticoid and guanylin (GN)/uroguanylin (UGN) expression in the colon. A murine model of chronic social defeat stress (CSDS) was established by exposing a C57BL/6N intruder mouse to a CD-1 aggressor mouse for 3–5 min. Thereafter the mice were kept in the same cage but separated by a divider for the remainder of the day. This procedure was repeated for 10 consecutive days, and then a social interaction test was performed to evaluate social avoidance. Fresh fecal and blood samples were collected for stool property analysis and measurement of the plasma glucocorticoid level by ELISA. The expression of GN, UGN, and guanylate cyclase 2C in the colonic tissues was examined by real-time RT-PCR and immunohistochemistry. Moreover, Lovo cells were stimulated with dexamethasone, and the expression of GN/UGN mRNA was examined. In the CSDS group, the time spent in the social interaction zone was significantly shorter when the CD-1 aggressor mouse was present than when it was absent. The social interaction ratio was also significantly lower in the CSDS group relative to the controls. The mean Bristol scale score was significantly lower in the CSDS group, but the fecal sodium concentration did not differ between CSDS mice and controls. The level of plasma corticosterone was significantly higher in the CSDS group than in the controls immediately after the 10th day of CSDS. The expression of both GN and UGN was significantly decreased in the CSDS mice. GN was expressed in all colonic epithelial cells, and UGN was expressed in ovoid or pyramidal epithelial cells in the colonic mucosa. The expression of both GN and UGN was significantly decreased in the CSDS mice relative to controls. The expression of both GN and UGN was significantly suppressed in Lovo cells upon stimulation with dexamethasone. Psychological stress-induced glucocorticoid may suppress colonic GN/UGN expression, resulting in a change in stool properties leading to constipation.
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spelling pubmed-77678432020-12-29 Decreased Colonic Guanylin/Uroguanylin Expression and Dried Stool Property in Mice With Social Defeat Stress Ebisutani, Nobuhiko Fukui, Hirokazu Nishimura, Heihachiro Nakanishi, Takashi Morimoto, Kenki Itou, Shiho Nakamura, Ayumi Masutani, Mizuki Hori, Mika Tomita, Toshihiko Oshima, Tadayuki Kasahara, Emiko Sekiyama, Atsuo Miwa, Hiroto Front Physiol Physiology Psychological stress is deeply involved in the pathophysiology of not only mental illness but also functional gastrointestinal disorders. In the present study, we examined the relationship between psychological stress and abnormality of stool properties, focusing on the alteration of plasma glucocorticoid and guanylin (GN)/uroguanylin (UGN) expression in the colon. A murine model of chronic social defeat stress (CSDS) was established by exposing a C57BL/6N intruder mouse to a CD-1 aggressor mouse for 3–5 min. Thereafter the mice were kept in the same cage but separated by a divider for the remainder of the day. This procedure was repeated for 10 consecutive days, and then a social interaction test was performed to evaluate social avoidance. Fresh fecal and blood samples were collected for stool property analysis and measurement of the plasma glucocorticoid level by ELISA. The expression of GN, UGN, and guanylate cyclase 2C in the colonic tissues was examined by real-time RT-PCR and immunohistochemistry. Moreover, Lovo cells were stimulated with dexamethasone, and the expression of GN/UGN mRNA was examined. In the CSDS group, the time spent in the social interaction zone was significantly shorter when the CD-1 aggressor mouse was present than when it was absent. The social interaction ratio was also significantly lower in the CSDS group relative to the controls. The mean Bristol scale score was significantly lower in the CSDS group, but the fecal sodium concentration did not differ between CSDS mice and controls. The level of plasma corticosterone was significantly higher in the CSDS group than in the controls immediately after the 10th day of CSDS. The expression of both GN and UGN was significantly decreased in the CSDS mice. GN was expressed in all colonic epithelial cells, and UGN was expressed in ovoid or pyramidal epithelial cells in the colonic mucosa. The expression of both GN and UGN was significantly decreased in the CSDS mice relative to controls. The expression of both GN and UGN was significantly suppressed in Lovo cells upon stimulation with dexamethasone. Psychological stress-induced glucocorticoid may suppress colonic GN/UGN expression, resulting in a change in stool properties leading to constipation. Frontiers Media S.A. 2020-12-14 /pmc/articles/PMC7767843/ /pubmed/33381053 http://dx.doi.org/10.3389/fphys.2020.599582 Text en Copyright © 2020 Ebisutani, Fukui, Nishimura, Nakanishi, Morimoto, Itou, Nakamura, Masutani, Hori, Tomita, Oshima, Kasahara, Sekiyama and Miwa. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Ebisutani, Nobuhiko
Fukui, Hirokazu
Nishimura, Heihachiro
Nakanishi, Takashi
Morimoto, Kenki
Itou, Shiho
Nakamura, Ayumi
Masutani, Mizuki
Hori, Mika
Tomita, Toshihiko
Oshima, Tadayuki
Kasahara, Emiko
Sekiyama, Atsuo
Miwa, Hiroto
Decreased Colonic Guanylin/Uroguanylin Expression and Dried Stool Property in Mice With Social Defeat Stress
title Decreased Colonic Guanylin/Uroguanylin Expression and Dried Stool Property in Mice With Social Defeat Stress
title_full Decreased Colonic Guanylin/Uroguanylin Expression and Dried Stool Property in Mice With Social Defeat Stress
title_fullStr Decreased Colonic Guanylin/Uroguanylin Expression and Dried Stool Property in Mice With Social Defeat Stress
title_full_unstemmed Decreased Colonic Guanylin/Uroguanylin Expression and Dried Stool Property in Mice With Social Defeat Stress
title_short Decreased Colonic Guanylin/Uroguanylin Expression and Dried Stool Property in Mice With Social Defeat Stress
title_sort decreased colonic guanylin/uroguanylin expression and dried stool property in mice with social defeat stress
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7767843/
https://www.ncbi.nlm.nih.gov/pubmed/33381053
http://dx.doi.org/10.3389/fphys.2020.599582
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